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Enzyme Nomenclature
Information on EC 3.2.1.113 - mannosyl-oligosaccharide 1,2-alpha-mannosidase
for references in articles please use BRENDA:EC3.2.1.113
Word Map on EC 3.2.1.113
- 3.2.1.113
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bipolar
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episode
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mood
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psychiatric
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psycho
- lithium
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schizophrenia
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antipsychotic
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mental
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antidepressant
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cognitive
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comorbidity
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anxiety
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double-blind
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adolescent
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monotherapy
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remission
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suicide
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psychopathology
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placebo
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outpatient
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social
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inpatient
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clinician
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dsm-iv
- valproate
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diagnoses
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psychosocial
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schizoaffective
- risperidone
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symptomatology
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anticonvulsant
- quetiapine
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emotional
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placebo-controlled
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youth
- hamilton
- olanzapine
- lamotrigine
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electroconvulsive
- carbamazepine
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neuroleptic
- drug development
- hallucinations
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panic
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extrapyramidal
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subscale
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treatment-emergent
- delirium
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psychotropic
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phenomenology
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
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EC NUMBER 
COMMENTARY 
3.2.1.113
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RECOMMENDED NAME
GeneOntology No.
mannosyl-oligosaccharide 1,2-alpha-mannosidase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2
Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2
mechanism: ordered equilibrium
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Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2
model for substrate and conformer selectivity, mechanism, computational study
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Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2
structure and function of enzyme from subgroups 1 to 3, overview
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Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2
structure and function of enzyme from subgroups 1 to 3, overview
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Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2
structure and function of enzyme from subgroups 1 to 3, overview
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Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2
structure and function of enzyme from subgroups 1 to 3, overview
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Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2
mechanism
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Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
hydrolysis of O-glycosyl bond
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
2-alpha-mannosyl-oligosaccharide alpha-D-mannohydrolase
Involved in the synthesis of glycoproteins.
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CAS REGISTRY NUMBER
COMMENTARY
9068-25-1
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain ATCC26555, CAI4, SC5314, NGY152, HMY5, HMY6
Uniprot
very similar (> 60%) to other fungal 1,2-alpha-mannosidases; silveira strain, formerly known as the non-California Coccidioides immitis
UniProt
intracellular protozoan, causes Chagas disease in humans, epimastigote stage
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
physiological function
malfunction
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blocking alpha-1,2-mannosidase in regulatory T cells results in the migration of significantly lower numbers to the peripheral lymph nodes in skin grafted mice following adoptive transfer
malfunction
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endoplasmic reticulum mannosidase I knockdown does not affect binding of an endoplasmic reticulum-associated degradation substrate glycoprotein to EDEM1
physiological function
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either isozyme MANIa or MANIb can function as the Golgi alpha-mannosidase I that produces the Man5GlcNAc2 N-glycan structure necessary for complex N-glycan synthesis
physiological function
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Htm1 protein generates the N-glycan signal for glycoprotein degradation in the endoplasmic reticulum. Htm1p physically interacts with Pdi1p via its C-terminal domain
physiological function
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class I alpha-mannosidases are essential for early N-glycan processing and are required for root development and cell wall biosynthesis in Arabidopsis thaliana
physiological function
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alpha-1,2-mannosidase function is not required for the suppressive capacity of regulatory T cells in vitro, but influences regulatory T cell adherence in vitro andmigration in vivo
physiological function
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endoplasmic reticulum mannosidase I activity is not required for association of H2a with EDEM1
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(Glc)1(Man)9(GlcNAc)2 + H2O
? + D-mannose
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removal of one alpha1,2-linked mannose
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(Glc)3(Man)9(GlcNAc)2 + H2O
? + D-mannose
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removal of one alpha1,2-linked mannose
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(Manalpha(1-2))nManalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3)) Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-3)Manbeta1-4GlcNAc beta1-4GlcNAc + D-mannose
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-
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?
2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose + H2O
2 alpha-D-mannose
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-
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?
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
4-methylumbelliferone + alpha-D-mannopyranoside
4-methylumbelliferyl-alpha-D-mannopyranoside
4-methylumbelliferone + D-mannose
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MUaMan, fluorogenic substrate
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?
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
4-methylumbelliferone + alpha-D-mannose
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?
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
4-methylumbelliferone + D-mannopyranose
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?
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
4-methylumbelliferone + D-mannose
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MUaMan fluorogenic substrate to measure enzyme activity
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?
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
? + D-mannose
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to measure enzyme activity
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?
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
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?
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
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?
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
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?
alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
? + D-mannose
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to measure enzyme activity
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?
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
Glcalpha(1-3)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Glcalpha(1-3)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
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high mannose chains of thyroglobulin and phytohemagglutinin-P + H2O
? + D-mannose
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thyroglobulin: 70% of alpha1,2-mannose residues accessible
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Man-alpha-1,2-Man-alpha-O-CH3 + H2O
methyl alpha-D-mannopyranoside + D-mannose
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Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNACbeta(1-4)GlcNAc + H2O
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
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the reaction is performed by ER-type alpha-mannosidase I protein MNS3
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-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNACbeta(1-4)GlcNAc + H2O
Manalpha(1-3)[Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNACbeta(1-4)GlcNAc + 4 D-mannose
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Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
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-
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?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
-
-
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 2 alpha-D-mannose
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?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 3 alpha-D-mannose
-
-
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-2)Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + 4 alpha-D-mannose
-
-
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
cytosolic isoform E-I
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-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
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Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-3)[Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNACbeta(1-4)GlcNAc + 3 D-mannose
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 2 H2O
(Man)5GlcNAc + 2 D-mannose
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-
-
-
?
Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + H2O
? + D-mannose
-
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)6(GlcNAc)2 + D-mannose
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-hexapeptide + H2O
(Man)6(GlcNAc)2-hexapeptide + D-mannose
-
substrate specificity not influenced by peptide moiety, removes 3 mannose residues
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2))Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-peptide + H2O
(Man)5(GlcNAc)-peptide + D-mannose
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
? + D-mannose
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
? + D-mannose
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNA + H2O
? + D-mannose
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
? + D-mannose
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
(Man)5(GlcNAc)2 + D-mannose
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)5(GlcNAc)2-Asn + D-mannose
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn + H2O
? + D-mannose
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
(Man)4GlcNAc + (Man)3GlcNAc + D-mannose
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)4(GlcNAc)2 + (Man)3(GlcNAc)2 + D-mannose
Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
-
-
-
?
Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
-
-
-
?
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
-
-
-
?
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-Asn + H2O
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta + D-mannose
methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside + H2O
? + D-mannose
used in a spectrophotometric assay
-
-
?
methyl-2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside + H2O
? + D-mannose
-
-
-
-
?
pyridylamino derivative of (Man)9(GlcNAc)2 + H2O
pyridylamino derivative of (Man)5(GlcNAc)2 + D-mannose
2-O-alpha-D-mannopyranosyl-D-mannose + H2O
D-mannose
-
i.e. alpha-D-Man-(1-2)-D-Man
-
-
?
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
4-methylumbelliferone + alpha-D-mannopyranoside
-
-
-
-
?
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
4-methylumbelliferone + alpha-D-mannopyranoside
-
-
-
-
?
4-nitrophenyl alpha-D-mannopyranoside + H2O
4-nitrophenol + alpha-D-mannopyranoside
-
-
-
-
?
4-nitrophenyl alpha-D-mannopyranoside + H2O
4-nitrophenol + alpha-D-mannopyranoside
-
-
-
-
?
alpha-1,2-linked mannotetraose + H2O
? + D-mannose
-
-
-
-
?
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
-
-
-
?
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
-
-
-
?
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
-
-
-
?
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
-
-
-
?
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
-
-
?
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
-
-
-
?
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
-
-
-
?
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
-
-
-
?
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
-
-
-
?
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
-
-
?
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
-
-
-
?
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
-
-
?
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
-
-
-
?
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
-
-
?
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
-
-
-
?
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
-
-
?
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
overexpression of each Golgi alpha1,2-mannosidase enhances alpha1-antitrypsin null degradation and trimming of its N-glycans to alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
-
?
alpha1-antitrypsin null + H2O
alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
overexpression of each Golgi alpha1,2-mannosidase enhances alpha1-antitrypsin null degradation and trimming of its N-glycans to alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose
-
-
?
baker's yeast mannan + H2O
? + D-mannose
-
releases 9.2% of total mannose
-
-
?
baker's yeast mannan + H2O
? + D-mannose
-
alpha-1,2-linked side-chains
-
-
?
baker's yeast mannan + H2O
? + D-mannose
-
alpha-1,2-linked side-chains
-
-
?
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-3)[Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNACbeta(1-4)GlcNAc + 3 D-mannose
-
-
-
-
-
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-3)[Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNACbeta(1-4)GlcNAc + 3 D-mannose
-
the reaction is performed by Golgi-alpha-mannosidase I proteins MNS1 and MNS2
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
i.e. (Man)9GlcNAc, specific for alpha-1,2-linked mannose residues
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
i.e. (Man)9GlcNAc, specific for alpha-1,2-linked mannose residues
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
product is Man6GlcNAc
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
product is Man8GlcNAc
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
products are Man5-8GlcNAc, with Man6GlcNAc being major product
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
function of enzyme from subgroup 1 to 3
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
pyridylamine-tagged Man9GlcNAc2
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
first product is Man6GlcNAc, followed by slower conversion to Man5GlcNAc
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
function of enzyme from subgroup 1 to 3
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
function of enzyme from subgroup 1 to 3
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
-
-
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
first product is Man6GlcNAc, followed by slower conversion to Man5GlcNAc
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
and (Man)6GlcNAc
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
best substrate
-
-
-
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
i.e. (Man)9GlcNAc, specific for alpha-1,2-linked mannose residues
and (Man)6GlcNAc
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
the terminal mannose on the middle antenna appears to be the most susceptible residue
-
-
-
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
removes all 4 alpha1,2-linked mannosyl residues
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
and (Man)6GlcNAc
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
i.e. (Man)9GlcNAc, specific for alpha-1,2-linked mannose residues
and (Man)6GlcNAc
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
-
-
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
best substrate
-
-
-
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
-
-
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
mutant R273L
product of mutant R273L, product of wild type is Man8GlcNAc
-
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
function of enzyme from subgroup 1 to 3
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
product is Man6GlcNAc
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
after long-term incubation, (Man)7GlcNAc and (Man)6GlcNAc after 4 h, (Man)8GlcNAc, (Man)7GlcNAc, (Man)6GlcNAc and (Man)5GlcNAc after 12 h incubation
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
removes all 4 alpha1,2-linked mannosyl residues
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)6(GlcNAc)2 + D-mannose
-
highly active on alpha(1-2) linked mannooligosaccharides
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)6(GlcNAc)2 + D-mannose
-
removes 3 of the 4 alpha1,2-linked mannosyl residues
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)6(GlcNAc)2 + D-mannose
-
i.e. (Man)9(GlcNAc)2
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)6(GlcNAc)2 + D-mannose
-
enzyme from endoplasmic reticulum removes only 1 or 2 alpha1,2-linked mannose residues
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)6(GlcNAc)2 + D-mannose
-
removes 3 of the 4 alpha1,2-linked mannosyl residues
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)6(GlcNAc)2 + D-mannose
-
removes 3 of the 4 alpha1,2-linked mannosyl residues
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)6(GlcNAc)2 + D-mannose
-
highly active on alpha(1-2) linked mannooligosaccharides
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)6(GlcNAc)2 + D-mannose
-
intact chitobiose core affects Man9-mannosidase specificity: reduction or removal of terminal N-acetylglucosamine residue increases hydrolytic susceptibility of the fourth alpha1,2-mannosyl-linkage
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2))Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-peptide + H2O
(Man)5(GlcNAc)-peptide + D-mannose
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2))Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-peptide + H2O
(Man)5(GlcNAc)-peptide + D-mannose
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
? + D-mannose
-
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
? + D-mannose
-
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
? + D-mannose
-
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
? + D-mannose
-
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNA + H2O
? + D-mannose
-
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNA + H2O
? + D-mannose
-
hydrolysis at 39% (mannosidase IB) or 67% (mannosidase IA) the rate of (Man)9GlcNAc hydrolysis
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNA + H2O
? + D-mannose
-
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNA + H2O
? + D-mannose
-
hydrolysis at 39% (mannosidase IB) or 67% (mannosidase IA) the rate of (Man)9GlcNAc hydrolysis
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
? + D-mannose
-
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
? + D-mannose
-
-
-
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
(Man)5(GlcNAc)2 + D-mannose
-
as in Taka-amylase A
-
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
(Man)5(GlcNAc)2 + D-mannose
-
pyridylamino derivative of Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-3)ManbetaGlcNAc2
i.e. pyridylamino derivative of (Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
(Man)5(GlcNAc)2 + D-mannose
-
preferred substrate
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
(Man)5(GlcNAc)2 + D-mannose
-
very poor substrate
-
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)5(GlcNAc)2-Asn + D-mannose
-
i.e. GPIV
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)5(GlcNAc)2-Asn + D-mannose
-
i.e. GPIV
major product formed, 3.5 mol mannose/mol GP IV per h, i.e. GPI
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn + H2O
? + D-mannose
-
-
-
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn + H2O
? + D-mannose
-
hydrolysis at 18-25% the rate of (Man)9GlcNAc hydrolysis
-
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn + H2O
? + D-mannose
-
hydrolysis at 18-25% the rate of (Man)9GlcNAc hydrolysis
-
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
(Man)4GlcNAc + (Man)3GlcNAc + D-mannose
-
without alpha1,2-linked mannose residues: poor substrate
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O
(Man)4GlcNAc + (Man)3GlcNAc + D-mannose
-
without alpha1,2-linked mannose residues: poor substrate
-
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)4(GlcNAc)2 + (Man)3(GlcNAc)2 + D-mannose
-
i.e. Manalpha(1-2)Manalpha(1-2)Manalpha(1-3) (Manalpha(1-6))ManbetaGlcNAc-betaGlcNAc
-
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)4(GlcNAc)2 + (Man)3(GlcNAc)2 + D-mannose
-
without alpha-1,2-linked mannose residues: no substrate
-
-
-
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
(Man)4(GlcNAc)2 + (Man)3(GlcNAc)2 + D-mannose
-
i.e. Manalpha(1-2)Manalpha(1-2)Manalpha(1-3) (Manalpha(1-6))ManbetaGlcNAc-betaGlcNAc
-
?
Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
-
-
-
?
Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
-
-
-
-
?
Manalpha(1->2)Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + H2O
Manalpha(1->2)Manalpha(1->3)[Manalpha(1->2)Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine + D-mannose
-
-
-
-
?
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-Asn + H2O
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta + D-mannose
-
-
-
-
?
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-Asn + H2O
Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta + D-mannose
-
-
-
-
?
p-nitrophenyl-alpha-D-mannoside + H2O
4-nitrophenol + D-mannose
-
mannosidase IB: poor substrate, mannosidase IA: no substrate
-
-
?
pyridylamino derivative of (Man)9(GlcNAc)2 + H2O
pyridylamino derivative of (Man)5(GlcNAc)2 + D-mannose
-
-
product is pyridylamino derivative of (Man)8(GlcNAc)2
?
pyridylamino derivative of (Man)9(GlcNAc)2 + H2O
pyridylamino derivative of (Man)5(GlcNAc)2 + D-mannose
-
-
after 120 h incubation, via specific intermediates: overview
?
additional information
?
-
-
methyl-3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside is not cleaved by MNS1, MNS2, and MNS3
-
-
-
additional information
?
-
-
no substrate: 3-O-alpha- and 6-O-alpha-D-mannobioses
-
-
-
additional information
?
-
-
no activity with p-nitrophenyl-alpha-mannoside, Manalpha(1-3)Man-OMe or Manalpha(1-6)Man-OMe
-
-
-
additional information
?
-
-
no substrate: 1,2-alpha-D-mannobiitol
-
-
-
additional information
?
-
-
no substrate: 1,2-alpha-D-mannobiitol
-
-
-
additional information
?
-
-
little or no activity with 4-methylumbelliferyl-alpha-mannoside, aryl-alpha-D-mannosides
-
-
-
additional information
?
-
-
enzyme is involved in the processing of N-linked mannans
-
-
-
additional information
?
-
enzyme is involved in the processing of N-linked mannans
-
-
-
additional information
?
-
-
cytosolic isoform E-I trims free Man8GlcNac2 isomer B into Man7GlcNac2 isomer B
-
-
-
additional information
?
-
cytosolic isoform E-I trims free Man8GlcNac2 isomer B into Man7GlcNac2 isomer B
-
-
-
additional information
?
-
-
effect of the alpha-1,2-mannosidic linkage in the alpha-1,3-branch of Man6GlcNAc2 on enzyme activity
-
-
-
additional information
?
-
-
substrate specificity and function of enzyme from subgroups 1 to 3, overview
-
-
-
additional information
?
-
-
generating the signal that targets misfolded glycoproteins for endoplasmic reticulum-associated protein degradation (ERAD)
-
-
-
additional information
?
-
-
targets misfolded glycoproteins for dislocation into the cytosol and destruction by 26 S proteasomes
-
-
-
additional information
?
-
-
when being targeted to endoplasmic reticulum-associated degradation, a misfolded glycoprotein undergoes endoplasmic reticulum mannosidase I-dependent trimming to yield the N-linked oligosaccharide structures Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine and Manalpha(1->3)[Manalpha(1->6)[Manalpha(1->2)Manalpha(1->3)]Manalpha(1->6)]Manbeta(1->4)GlcNAcbeta(1->4)GlcNAcbeta-2-pyridylamine
-
-
-
additional information
?
-
-
the enzyme does not hydrolyze alpha-D-Manp-(1-3)-D-Manp and alpha-D-Manp-(1-6)-D-Manp
-
-
-
additional information
?
-
-
the enzyme does not hydrolyze alpha-D-Manp-(1-3)-D-Manp and alpha-D-Manp-(1-6)-D-Manp
-
-
-
additional information
?
-
-
substrate specificity and function of enzyme from subgroups 1 to 3, overview
-
-
-
additional information
?
-
generating the signal that targets misfolded glycoproteins for endoplasmic reticulum-associated protein degradation (ERAD)
-
-
-
additional information
?
-
generating the signal that targets misfolded glycoproteins for endoplasmic reticulum-associated protein degradation (ERAD)
-
-
-
additional information
?
-
-
substrate specificity and function of enzyme from subgroups 1 to 3, overview
-
-
-
additional information
?
-
-
mannosidase IA and B are quite similar in substrate specificity
-
-
-
additional information
?
-
-
mannosidase IA and B are quite similar in substrate specificity
-
-
-
additional information
?
-
-
substrate specificity and function of enzyme from subgroups 1 to 3, overview
-
-
-
additional information
?
-
-
the Htm1p exomannosidase activity requires processing of the N-glycan by glucosidase I, glucosidase II, and mannosidase I
-
-
-
additional information
?
-
-
no substrate: 4-methylumbelliferyl-alpha-mannoside
-
-
-
additional information
?
-
-
no substrate: 4-methylumbelliferyl-alpha-mannoside
-
-
-
additional information
?
-
-
pig liver enzyme substrate specificity resembles that of calf liver enzyme
-
-
-
additional information
?
-
-
no hydrolysis of alpha1,6-linkages
-
-
-
additional information
?
-
-
more specific towards Man-alpha-1,2-bonds, but also hydrolysis of alpha-1,3- and of alpha-1,6-bonds
-
-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + D-mannose
Q8J0Q0
cytosolic isoform E-I
-
-
?
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 2 H2O
(Man)5GlcNAc + 2 D-mannose
-
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
-
-
-
?
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + H2O
alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,3)-[alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,6)]-alpha-D-mannopyranosyl-(1,6)]-beta-D-mannopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranose + D-mannose
-
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
function of enzyme from subgroup 1 to 3
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
function of enzyme from subgroup 1 to 3
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
function of enzyme from subgroup 1 to 3
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
-
-
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
and (Man)6GlcNAc
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
and (Man)6GlcNAc
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
-
-
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
-
-
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
function of enzyme from subgroup 1 to 3
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
(Man)5GlcNAc + 4 D-mannose
-
-
-
?
additional information
?
-
-
enzyme is involved in the processing of N-linked mannans
-
-
-
additional information
?
-
Q8J0Q0
enzyme is involved in the processing of N-linked mannans
-
-
-
additional information
?
-
-
generating the signal that targets misfolded glycoproteins for endoplasmic reticulum-associated protein degradation (ERAD)
-
-
-
additional information
?
-
-
targets misfolded glycoproteins for dislocation into the cytosol and destruction by 26 S proteasomes
-
-
-
additional information
?
-
P39098
generating the signal that targets misfolded glycoproteins for endoplasmic reticulum-associated protein degradation (ERAD)
-
-
-
additional information
?
-
P45700
generating the signal that targets misfolded glycoproteins for endoplasmic reticulum-associated protein degradation (ERAD)
-
-
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Co2+
Divalent cations
Mg2+
Mn2+
Zn2+
additional information
Ca2+
-
required for activity, isozyme MANIa shows 450% relative activity at 15 mM Ca2+, isozyme MANIb shows 600% relative activity at 15 mM Ca2+
Ca2+
required for activity. Optimum concentration 1.5 mM or 15 mM. Isoform ManIC is stable in the absence of Ca2+, even though Ca2+ is also effective for activity; required for activity. Optimum concentration 15 mM. the activity of soform ManIA2 is significantly decreased after incubation at 30°C without Ca2+, which is about 30% compared with the activity of the enzyme after incubation at 5°C. The enzyme activity of ManIA2 increases with increasing Ca2+ concentration until 12 mM
Ca2+
the enzyme assumes an (alphaalpha)7 barrel structure with a Ca2+ ion coordinated at the bas of the barrel
Ca2+
-
requirement, protects from thermal inactivation, Km value 0.5 mM
Zn2+
the activity of isoform ManIC is 94% in the presence of 15 mM Zn2+ and 50% in the presence of 1.5 mM Zn2+ compared with that of the original solution
additional information
-
Co2+, Cu2+, Mg2+, Mn2+, and Zn2+ have no effect on activity
additional information
-
although MNS1, MNS2, and MNS3 do not display any specific preference for Ca2+ over Mn2+, the activity is restored to a much lower degree in the presence of Mg2+
additional information
isoform ManIA2 shows no activity in the presence of Co2+, Mn2+, and Cu2+; isoform ManIC shows no activity in the presence of Co2+ and Mn2+
additional information
isoform ManIA2 shows no activity in the presence of Co2+, Mn2+, and Cu2+; isoform ManIC shows no activity in the presence of Co2+ and Mn2+
additional information
-
the enzyme activity os not sensitive to the presence of Ca2+, Mg2+ and Mn2+ at concentrations up to 3 mM
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1-Cyclohexyl-3-(2-morpholinyl-4-ethyl)carbodiimide/glycine methylester
-
-
2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
-
in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
-
in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose
-
6-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
-
in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose
brefeldin A
-
known inhibitor of vesicle transport, ablated intracellular turnover of the radiolabeled molecules. Plus digestion with potato acid phosphatase implies that phosphorylation of ER mannosidase I is responsible for the altered electrophoretic mobility
D-mannonolactam amidrazone
-
mannosidase inhibitor, MDCK-cells: in vivo and in vitro
deoxymannojirimycin hydrochloride
inhibitor at a final concentration of 0.5 mM, reduces the activity by 80%
Mannosyl-mannose disaccharide
-
in decreasing order of efficiency: alpha1,2, alpha 1,3 and alpha1,6-mannosylmannose
-
methyl-alpha-D-lyxopyranosyl-(1',2)-alpha-D-mannopyranoside
analogue of the minimal disaccharide substrate mannobiose. Cocrystallization and mass spectrometry data suggest that when methyl-alpha-D-lyxopyranosyl-(1',2)-alpha-D-mannopyranoside is incubated with the mannosidase for long periods of time it is cleaved and that methyl-alpha-D-lyxopyranosyl-(1',2)-alpha-D-mannopyranoside behaves as a substrate
N-carbobenzoxyl-leucinyl-leucinyl-leucinal
-
degradation is inhibited by the proteasomal inhibitor
pepstatin A
-
in the presence of mixed membrane fraction from strain ATTC 26555, the 52 kDa polypeptide is converted into a 43 kDa active product. Protease inhibitors, such as phenylmethylsulphonylfluoride, trans-epoxysuccinyl-L-leucylamido(4-guanidino)butane and 1,10-phenantroline, fail to prevent proteolysis. Pepstatin A, an aspartyl protease inhibitor, fully blocked enzyme processing
1-deoxymannojirimycin
-
isozyme MANIa shows 20% residual activity at 0.1 mM 1-deoxymannojirimycin, isozyme MANIb shows 45% residual activity at 0.1 mM 1-deoxymannojirimycin
1-deoxymannojirimycin
-
trimming is inhibited preferentially by 1-deoxymannojirimycin, inhibits hydrolysis of 4-methylumbelliferyl-alpha-D-mannopyranoside
1-deoxymannojirimycin
-
DMJ, inhibition of 4-methylumbelliferyl-alpha-D-mannopyranoside hydrolysis
1-deoxymannojirimycin
-
mannose analogue, specific inhibitor of alpha1,2-mannosidase. Focus on the effect of blocking alpha-mannosidases I in human hepatocarcinoma 7721 cells concerning with endoplasmic reticulum stress. Observed that 1-deoxymannojirimycin can also be a radical stimulus in inducing endoplasmic reticulum stress
1-deoxymannojirimycin
no activity at 0.01 mM; no activity at 0.01 mM
1-deoxymannojirimycin
-
mannose analog, in vivo and in vitro; reversible (not by Ca2+)
1-deoxymannojirimycin
-
mannose analog, in vivo and in vitro; rat liver enzyme
1-deoxymannojirimycin
-
found in crystal structures of the active site
1-deoxymannojirimycin
-
the enzyme is inhibited preferentially by 1-deoxymannojirimycin
Chloroquine
-
lysosomotrophic amine capable of raising the pH of acidic intracellular compartments, added to the medium with weak inhibition
Chloroquine
-
lysosomotrophic amine capable of raising the pH of acidic intracellular compartments, effect on the intracellular fate of endogenous mouse ER mannosidase I
Co2+
-
50% reduction in alpha-mannosidase activity is observed after addition of 3 mM CoCl2
EDTA
-
isozyme MANIa shows 20% residual activity at 1 mM EDTA, isozyme MANIb shows 35% residual activity at 1 mM EDTA, isozyme MANIb activity in the presence of 1 mM EDTA is restored by the addition of Ca2+
EDTA
-
enzyme loses 96% activity after dialysis in 50 mM PIPES buffer, pH 6.0, containing 2 mM EDTA. Addition of 10 mM Ca2+ recovers activity to 100%, addition of 10 mM Mg2+ recovers activity to 43%
EDTA
-
complete inactivition, Zn2+ restores, Ca2+, Cd2+, Mg2+, Ni2+, Na+ or Cu2+ restore partly
EGTA
-
ethylene glycol-bis(beta-aminoethylether)-N,N,N',N'-tetraacetic acid
kifunensine
-
inhibits Golgi alpha1,2-mannosidase. Addition of kifunensine completely inhibits mannose trimming from alpha1-antitrypsin null (Hong Kong) in mock transfected cells. Kifunensine inhibits alpha1-antitrypsin null (Hong Kong) degradation in cells co-transfected with the Golgi mannosidases
kifunensine
-
general membrane-permeable inhibitor of alpha-mannosidase activity
kifunensine
inhibits Golgi alpha1,2-mannosidase. Addition of kifunensine completely inhibits mannose trimming from alpha1-antitrypsin null (Hong Kong) in mock transfected cells. Kifunensine inhibits alpha1-antitrypsin null (Hong Kong)degradation in cells co-transfected with the Golgi mannosidases; inhibits Golgi alpha1,2-mannosidase. Addition of kifunensine completely inhibits mannose trimming from alpha1-antitrypsin null (Hong Kong) in mock transfected cells. Kifunensine inhibits alpha1-antitrypsin null (Hong Kong)degradation in cells co-transfected with the Golgi mannosidases
kifunensine
-
general membrane-permeable inhibitor of alpha-mannosidase activity, effect on the intracellular fate of endogenous mouse ER mannosidase I
lactacystin
-
irreversible inhibitor of multicatalytic proteasomes, only a minor effect on the disappearance of radiolabeled human ER mannosidase I
lactacystin
-
irreversible inhibitor of multicatalytic proteasomes, effect on the intracellular fate of endogenous mouse ER mannosidase I
leupeptin
-
lysosomal protease inhibitor, intracellular turnover of pulse-radiolabeled recombinant human ER mannosidase I is substantially inhibited
leupeptin
-
lysosomal protease inhibitor, effect on the intracellular fate of endogenous mouse ER mannosidase I
swainsonine
-
inhibits hydrolysis of 4-methylumbelliferyl-alpha-D-mannopyranoside
swainsonine
-
SW, inhibition of 4-methylumbelliferyl-alpha-D-mannopyranoside hydrolysis
swainsonine
isoform ManIC shows 86% activity at 0.5 mM swainsonine
Zn2+
-
50% reduction in alpha-mannosidase activity is observed after addition of 3 mM ZnCl2
additional information
-
MNS1, MNS2, and MNS3 are not sensitive to inhibition by swainsonine
-
additional information
-
no inhibition by 4-nitrophenyl-alpha-mannoside, mannitol, L-mannono-1,4-lactone, PMSF
-
additional information
-
staurosporine and genistein have an effect, tesing arrested intracellular turnover in response to selective inhibition of serine kinase activity
-
additional information
-
shift in migration is due to mannose trimming. Effects are not observed with the class II mannosidase inhibitor swainsonine
-
additional information
-
cycloheximide, protein synthesis inhibitor. After a 2 h treatment with the protein synthesis inhibitor cycloheximide, ER and Golgi labeling observed with ManI-GFP fusion remains unchanged, showing that the steady state localization of ManI-GFP is the Golgi and the ER
-
additional information
comparison with 1-deoxymannojirimycin and kifunensine
-
additional information
-
mannosidase IA and B are quite similar in response to inhibitors
-
additional information
-
no inhibition by swainsonine; no or little inhibition by N,N-dimethyl-1-deoxymannojirimycin
-
additional information
-
no inhibition by mannose, alpha1,6-linked mannose oligosaccharide, castanospermine, deoxynojirimycin, 2,5-dihydroxymethyl-3,4-dihydroxypyrrolidine
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
phosphatidylcholine
-
activation, with acyl-chains of different lengths, above C-4
phosphatidylethanolamine
-
activation, in the presence of 3.5 mM Triton X-100
Zwitterionic phospholipids
-
activation, required for solubilized and purified enzyme, independent of acyl chain length or degree of saturation, an ordered lipid structure of either micelles or bilayers, mixed micelles together with Triton X-100
-
additional information
-
no activation by negatively charged phospholipids, dibutyryl-phosphocholine
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.04
4-methylumbelliferyl alpha-D-mannopyranoside
-
at pH 7.0 in 50 mM MES-Tris buffer and at 37°C
0.015 - 0.51
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
0.068 - 0.26
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
additional information
additional information
-
kinetic constants of various substrate derivatives
-
0.09
4-methylumbelliferyl-alpha-D-mannopyranoside
-
hydrolysis of the fluorogenic substrate follows hyperbolic kinetics
0.11
4-methylumbelliferyl-alpha-D-mannopyranoside
-
MUaMan, fluorogenic substrate. Vmax 36.2 nmol of 4-methylumbelliferone min-1 mg-1 of protein
0.719
Manalpha(1,2)Man-O-Me
-
pH 5.0, 30°C, mutant enzyme C443A; pH 5.0, 30°C, wild-type enzyme
0.015
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.5, mutant enzyme T688A
0.068
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 5.3, mutant enzyme E330Q
0.11
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 7.0, wild-type enzyme
0.12
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.8, mutant enzyme F659A
0.33
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.5, mutant enzyme R461L
0.47
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.5, mutant enzyme R597A
0.51
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.5, mutant enzyme R461A
0.068
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 5.3, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E330Q
0.09
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 7.1, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E330Q
0.11
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.8, pyridylamine-tagged Man9GlcNAc2, mutant enzyme D463N; 37°C, pH 7.1, pyridylamine-tagged Man9GlcNAc2, wild-type enzyme
0.15
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.6, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E330Q/E599Q
0.16
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.7, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E599Q
0.21
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.9, pyridylamine-tagged Man9GlcNAc2, mutant enzyme H524A
0.26
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.6, pyridylamine-tagged Man9GlcNAc2, mutant enzyme D463N/E599Q
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.03 - 3.7
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
0.000026 - 3.7
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
0.03
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.8, mutant enzyme F659A
0.06
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.5, mutant enzyme T688A
0.07
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.5, mutant enzyme R461L
0.084
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 5.3, mutant enzyme E330Q
0.11
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.5, mutant enzyme R597A
0.6
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.5, mutant enzyme R461A
3.7
Manalpha(1-2)Manalpha(1-3)Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 7.0, wild-type enzyme
0.000026
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.7, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E599Q
0.000027
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.6, pyridylamine-tagged Man9GlcNAc2, mutant enzyme D463N/E599Q
0.00028
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.6, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E330Q/E599Q
0.0046
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.8, pyridylamine-tagged Man9GlcNAc2, mutant enzyme D463N
0.018
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 7.1, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E330Q
0.084
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 5.3, pyridylamine-tagged Man9GlcNAc2, mutant enzyme E330Q
0.86
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 6.9, pyridylamine-tagged Man9GlcNAc2, mutant enzyme H524A
3.7
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc
-
37°C, pH 7.1, pyridylamine-tagged Man9GlcNAc2, wild-type enzyme
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.6
methyl-alpha-D-lyxopyranosyl-(1',2)-alpha-D-mannopyranoside
-
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.2
1-deoxymmanojirimycin
Candida albicans
-
inhibits hydrolysis of 4-methylumbelliferyl-alpha-D-mannopyranoside
-
0.03
1-deoxymannojirimycin
Arabidopsis thaliana
-
class I alpha-mannosidase MNS1, using methyl-2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside as substrate
0.035
1-deoxymannojirimycin
Arabidopsis thaliana
-
class I alpha-mannosidase MNS3, using methyl-2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside as substrate
0.04
1-deoxymannojirimycin
Arabidopsis thaliana
-
class I alpha-mannosidase MNS2, using methyl-2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside as substrate
0.12
1-deoxymannojirimycin
Sporothrix schenckii
-
at pH 7.0 in 50 mM MES-Tris buffer and at 37°C
0.23
1-deoxymannojirimycin
Candida albicans
-
inhibition of 4-methylumbelliferyl-alpha-D-mannopyranoside hydrolysis
0.0003
kifunensine
Arabidopsis thaliana
-
class I alpha-mannosidase MNS3, using methyl-2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside as substrate
0.00035
kifunensine
Arabidopsis thaliana
-
class I alpha-mannosidase MNS1, using methyl-2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside as substrate
0.00047
kifunensine
Arabidopsis thaliana
-
class I alpha-mannosidase MNS2, using methyl-2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside as substrate
0.47
swainsonine
Candida albicans
-
inhibition of 4-methylumbelliferyl-alpha-D-mannopyranoside hydrolysis
0.55
swainsonine
Candida albicans
-
inhibits hydrolysis of 4-methylumbelliferyl-alpha-D-mannopyranoside
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.00003
-
purification of soluble alpha-mannosidase from CAI-4 strain, High-speed supernatant used to measure alpha-mannosidase activity
0.00008
-
purification of soluble alpha-mannosidase from CAI-4 strain, Sepharose CL6B
0.0002
-
mixed membrane fraction (MMF) of ATCC 26555 supernatant, expressed as nanomol of methylumbelliferone min-1mg-1 of protein
0.0005
-
mixed membrane fraction (MMF) of CAI-4 and ATCC 26555 plus 1 mM pepstatin supernatant, expressed as nanomol of methylumbelliferone min-1mg-1 of protein; mixed membrane fraction (MMF) of CAI-4 supernatant, expressed as nanomol of methylumbelliferone min-1mg-1 of protein
0.0012
-
mixed membrane fraction (MMF) of CAI-4 and ATCC 26555 pellet, expressed as nanomol of methylumbelliferone min-1mg-1 of protein
0.0019
-
mixed membrane fraction (MMF) of ATCC 26555 pellet, expressed as nanomol of methylumbelliferone min-1mg-1 of protein
0.0021
-
purification of soluble alpha-mannosidase from CAI-4 strain, DEAE Bio-Gel A
0.0022
-
mixed membrane fraction (MMF) of CAI-4 and ATCC 26555 plus 1 mM pepstatin pellet, expressed as nanomol of methylumbelliferone min-1mg-1 of protein
0.0026
-
mixed membrane fraction (MMF) of CAI-4 pellet, expressed as nanomol of methylumbelliferone min-1mg-1 of protein
0.003
-
purification of soluble alpha-mannosidase from CAI-4 strain, Sephadex G-25
0.0047
-
mixed membrane fraction (MMF) of CAI-4 and ATCC 26555 supernatant, expressed as nanomol of methylumbelliferone min-1mg-1 of protein
0.0057
-
purification of soluble alpha-mannosidase from CAI-4 strain, DEAE Bio-Gel A
0.0101
-
purification of soluble alpha-mannosidase from CAI-4 strain, Con A-Sepharose
0.0108
-
purification of soluble alpha-mannosidase from CAI-4 strain, Sephadex G-25
additional information
additional information
-
hydrolysis of the fluorogenic substrate 4-methylumbelliferyl-alpha-D-mannopyranoside follows hyperbolic kinetics and LineweaverBurk plots reveals Vmax values of 30.9 nmol of methylumbelliferone min-1 mg-1 of protein
additional information
-
modeling studies, molecular basis of difference in specificity, distinction of subgroup 1 and 2
additional information
-
modeling studies, molecular basis of difference in specificity, distinction of subgroup 1 and 2
additional information
-
model for substrate and conformer specificity, computational study
additional information
-
modeling studies, molecular basis of difference in specificity, distinction of subgroup 1 and 2
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5 - 6
5.5 - 6
6 - 6.5
6.5
6.8
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4 - 5.8
-
about half-maximal activity at pH 4 and 5.8, 0.1 M acetate buffer
5.2 - 7.3
-
about half-maximal activity at pH 5.2 and 7.3, in the presence of egg yolk lyso-phosphatidylcholine
5.5 - 7.5
-
about half-maximal activity at pH 5.5 and 7.5, in the presence of soybean phosphatidylinositol
5.5 - 7
-
about half-maximal activity at pH 5.5 and 7; (Man)8(GlcNAc)2-peptide as substrate
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
37
40
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
45 - 60
-
45°C: optimum, 60°C: 60% of maximal activity, 65°C: 14% of maximal activity, hydrolysis of 1,2-alpha-mannobiose
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
In situ hybridization shows high levels of alpha1,2-mannosidase IB
levels of Golgi alpha1,2-mannosidase IC are similar in most tissues, with the exception of placenta and liver
-
Taka-diastase, enzyme-product from Sankyo Co.
In situ hybridization shows high levels of alpha1,2-mannosidase IA; In situ hybridization shows high levels of alpha1,2-mannosidase IB
Golgi alpha1,2-mannosidase I displays distinct developmental and tissue-specific expression. Expression of Golgi alpha 1,2-mannosidases (IA, IB, IC) investigated by Nothern blot analysis and in situ hybridization; Golgi alpha1,2-mannosidase I displays distinct developmental and tissue-specific expression. Expression of Golgi alpha 1,2-mannosidases (IA, IB, IC) investigated by Nothern blot analysis and in situ hybridization. In situ hybridization shows high levels of alpha1,2-mannosidase IB; Golgi alpha1,2-mannosidase I displays distinct developmental and tissue-specific expression. Expression of Golgi alpha 1,2-mannosidases (IA, IB, IC) investigated by Nothern blot analysis and in situ hydridization
Golgi alpha1,2-mannosidase IA; Golgi alpha1,2-mannosidase IB
Golgi alpha1,2-mannosidase IA; In situ hybridization shows high levels of alpha1,2-mannosidase IB
Golgi alpha1,2-mannosidase IA; In situ hybridization shows high levels of alpha1,2-mannosidase IA; In situ hybridization shows high levels of alpha1,2-mannosidase IB, Golgi alpha1,2-mannosidase IB
Golgi alpha1,2-mannosidase IA; Golgi alpha1,2-mannosidase IB
additional information
detected in the coccidioidal T27K vaccine
Golgi alpha1,2-mannosidase IA; In situ hybridization shows high levels of alpha1,2-mannosidase IB; levels of Golgi alpha1,2-mannosidase IC are similar in most tissues, with the exception of placenta and liver
Golgi alpha1,2-mannosidase IA; In situ hybridization shows high levels of alpha1,2-mannosidase IA
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
with strain 22A1, the ratio of intracellular to extracellular 1,2-alpha-mannosidase activity is about 4:1
-
; presence of a 65000 Da membrane-bound isoform that is cleaved by protease Kex2 to soluble 52000 Da isoform E-I
-
-
with strain 22A1, the ratio of intracellular to extracellular 1,2-alpha-mannosidase activity is about 4:1
-
2 enzymes: a membrane-bound microsomal and a soluble cytoplasmic one
-
-
-
pericentriolar endoplasmic reticulum-derived quality control compartment
-
with the catalytic domain within the lumen of this compartment
-
with the catalytic domain within the lumen of this compartment
-
-
different fusion proteins analyzed to determine if the portion of ManI located in the Golgi lumen plays a role in the targeting of this glycosidase to the Golgi and the ER membranes
different fusion proteins analyzed to determine if the portion of ManI located in the Golgi lumen plays a role in the targeting of this glycosidase to the Golgi and the ER membranes
-
different fusion proteins analyzed to determine if the portion of ManI located in the Golgi lumen plays a role in the targeting of this glycosidase to the Golgi and the ER membranes
-
different fusion proteins analyzed to determine if the portion of ManI located in the Golgi lumen plays a role in the targeting of this glycosidase to the Golgi and the ER membranes
-
2 enzymes: a membrane-bound microsomal and a soluble cytoplasmic one
-
2 enzymes: a membrane-bound microsomal and a soluble cytoplasmic one
-
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PDB
SCOP
CATH
ORGANISM
UNIPROT
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
23000
-
analytical electrophoresis of the purified enzyme reveals two polypeptides of 52 and 23 kDa, 52000 Da polypeptide being responsible for enzyme activity as revealed by zymogram analysis
27000
-
analytical electrophoresis of the purified preparation reveals two polypeptides
43000
49000
52000
56920
theoretical molecular weight. SDS-PAGE, Western blot analysis. A highly purified fraction is obtained after FPLC
63000
65000
79000
-
Western blotting of cell lysates, transiently expressed recombinant human ER mannosidase I is absent from mock transfected cells but detected as a single immunoreactive 79 kDa band following transient expression
82000