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Literature summary for 3.2.1.113 extracted from

  • Tulsiani, D.R.P.; Hubbard, S.C.; Robbins, P.W.; Touster, O.
    alpha-D-Mannosidases of rat liver Golgi membranes. Mannosidase II is the GlcNAcMAN5-cleaving enzyme in glycoprotein biosynthesis and mannosidases Ia and IB are the enzymes converting Man9 precursors to Man5 intermediates (1982), J. Biol. Chem., 257, 3660-3668.
    View publication on PubMed

General Stability

General Stability Organism
mannosidase IA and B differ in sensitivity to storage in liver cytoplasmic extracts, no interconversion between mannosidase IA and IB during cellulose phosphate chromatography Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
additional information mannosidase IA and B are quite similar in response to inhibitors Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane
-
Rattus norvegicus 139
-
membrane
-
Rattus norvegicus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O Rattus norvegicus
-
(Man)5GlcNAc + 4 D-mannose
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O Rattus norvegicus Wistar
-
(Man)5GlcNAc + 4 D-mannose
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
male
-
Rattus norvegicus
-
Wistar
-
Rattus norvegicus Wistar
-
Wistar
-

Purification (Commentary)

Purification (Comment) Organism
partial Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
2-4°C, in 10 mM potassium phosphate buffer, pH 7.2, 1% Triton X-100, 20% loss within 4 weeks Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GlcNAc-(Man)5GlcNAc + H2O poor substrate Rattus norvegicus ? + D-mannose
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
-
Rattus norvegicus (Man)5GlcNAc + 4 D-mannose
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O best substrate Rattus norvegicus (Man)5GlcNAc + 4 D-mannose
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
-
Rattus norvegicus Wistar (Man)5GlcNAc + 4 D-mannose
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O best substrate Rattus norvegicus Wistar (Man)5GlcNAc + 4 D-mannose
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNA + H2O hydrolysis at 39% (mannosidase IB) or 67% (mannosidase IA) the rate of (Man)9GlcNAc hydrolysis Rattus norvegicus ? + D-mannose
-
?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNA + H2O hydrolysis at 39% (mannosidase IB) or 67% (mannosidase IA) the rate of (Man)9GlcNAc hydrolysis Rattus norvegicus Wistar ? + D-mannose
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn + H2O hydrolysis at 18-25% the rate of (Man)9GlcNAc hydrolysis Rattus norvegicus ? + D-mannose
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn + H2O hydrolysis at 18-25% the rate of (Man)9GlcNAc hydrolysis Rattus norvegicus Wistar ? + D-mannose
-
?
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O without alpha1,2-linked mannose residues: poor substrate Rattus norvegicus (Man)4GlcNAc + (Man)3GlcNAc + D-mannose
-
?
additional information mannosidase IA and B are quite similar in substrate specificity Rattus norvegicus ?
-
?
additional information mannosidase IA and B are quite similar in substrate specificity Rattus norvegicus Wistar ?
-
?
p-nitrophenyl-alpha-D-mannoside + H2O mannosidase IB: poor substrate, mannosidase IA: no substrate Rattus norvegicus 4-nitrophenol + D-mannose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
mannosidase IA and B differ in thermostability Rattus norvegicus
37
-
24 h, in 0.1 M sodium acetate buffer, pH 5.8, 30% loss of activity Rattus norvegicus
50
-
mannosidase IB less stable than IA Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
mannosidase IA and B Rattus norvegicus