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Literature summary for 3.2.1.113 extracted from

  • Schweden, J.; Legler, G.; Bause, E.
    Purification and characterization of a neutral processing mannosidase from calf liver acting on (Man)9(GlcNAc)2 oligosaccharides (1986), Eur. J. Biochem., 157, 563-570.
    View publication on PubMed
Search for more literature for 3.2.1.113

Inhibitors

Inhibitors Comment Organism Structure
1-deoxymannojirimycin
-
 Bos taurus
Cu2+
-
 Bos taurus
EDTA Ca2+ restores Bos taurus
N-5-Carboxypentyldeoxymannojirimycin
-
 Bos taurus
N-Methyl-deoxymannojirimycin
-
 Bos taurus
Ni2+
-
 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Bos taurus 5783
-
membrane
-
 Bos taurus 16020
-
microsome
-
 Bos taurus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
divalent cations requirement Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 x * 56000, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Glc)1(Man)9(GlcNAc)2 + H2O removal of one alpha1,2-linked mannose Bos taurus ? + D-mannose
-
 ?
(Glc)3(Man)9(GlcNAc)2 + H2O removal of one alpha1,2-linked mannose Bos taurus ? + D-mannose
-
 ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O removes 3 of the 4 alpha1,2-linked mannosyl residues Bos taurus (Man)6(GlcNAc)2 + D-mannose
-
 ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Bos taurus ? + D-mannose
-
 ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Bos taurus ? + D-mannose
-
 ?
additional information little or no activity with 4-methylumbelliferyl-alpha-mannoside, aryl-alpha-D-mannosides Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.2
-
 Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7 about half-maximal activity at pH 5.5 and 7 Bos taurus