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Literature summary for 3.2.1.113 extracted from

  • Yoshida, T.; Kato, Y.; Asada, Y.; Nakajima, T.
    Filamentous fungus Aspergillus oryzae has two types of alpha-1,2-mannosidases, one of which is a microsomal enzyme that removes a single mannose residue from Man9GlcNAc2 (2000), Glycoconj. J., 17, 745-748 .
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
EDTA complete inhibition at 10 mM Aspergillus oryzae
kifunensine
-
 Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Aspergillus oryzae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10 mM used in assay conditions Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Man9GlcNAc2-pyridylamine + H2O Aspergillus oryzae
-
 Man8GlcNAc2-pyridylamine (isomer 8A1,2,3B1,3) + D-mannose
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Man9GlcNAc2-pyridylamine + H2O
-
 Aspergillus oryzae Man8GlcNAc2-pyridylamine (isomer 8A1,2,3B1,3) + D-mannose
-
 ?

Synonyms

Synonyms Comment Organism
alpha-1,2-mannosidase
-
 Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 in 100 mM PIPES buffer Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 41% activity at pH 5.5, 100% activity at pH 7.0, and 18% activity at pH 8.0 Aspergillus oryzae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00005 0.0001 at pH 7.0 and 30°C Aspergillus oryzae kifunensine