Literature summary for 3.2.1.113 extracted from

Mora-Montes, H.; Lopez-Romero, E.; Zinker, S.; Ponce-Noyola, P.; Flores-Carreon, A.
Purification of soluble alpha1,2-mannosidase from Candida albicans CAI-4 (2006), FEMS Microbiol. Lett., 256, 50-56 .

Search for more literature for 3.2.1.113

Inhibitors

Inhibitors	Comment	Organism	Structure
1-deoxymannojirimycin	-	Candida albicans
swainsonine	-	Candida albicans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.09	-	4-methylumbelliferyl alpha-D-mannopyranoside	at pH 6.0 in 50 mM Tris-MES and at 37°C	Candida albicans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	-	Candida albicans	-	-

Organism

Organism	UniProt	Comment	Textmining
Candida albicans	-	-	-
Candida albicans CAI-4	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Sepharose CL6B column chromatography, DEAE Bio-Gel A column chromatography, Concanavalin A-Sepharose column chromatography, and Sephadex G-25 gel filtration	Candida albicans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl alpha-D-mannopyranoside + H2O	-	Candida albicans	4-methylumbelliferone + alpha-D-mannopyranose	-	?
4-methylumbelliferyl alpha-D-mannopyranoside + H2O	-	Candida albicans CAI-4	4-methylumbelliferone + alpha-D-mannopyranose	-	?
Man9GlcNAc2-Asn + 4 H2O	-	Candida albicans	Man5GlcNAc2-Asn + 4 beta-D-mannose	-	?
Man9GlcNAc2-Asn + 4 H2O	-	Candida albicans CAI-4	Man5GlcNAc2-Asn + 4 beta-D-mannose	-	?
Man9GlcNAc2-Asn + H2O	-	Candida albicans	Man8GlcNAc2-Asn (isomer 8A1,2,3B1,3) + D-mannose	-	?
Man9GlcNAc2-Asn + H2O	-	Candida albicans CAI-4	Man8GlcNAc2-Asn (isomer 8A1,2,3B1,3) + D-mannose	-	?

Subunits

Subunits	Comment	Organism
?	x * 52000, SDS-PAGE	Candida albicans

Synonyms

Synonyms	Comment	Organism
alpha1,2-mannosidase	-	Candida albicans

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.23	-	at pH 6.0 in 50 mM Tris-MES and at 37°C	Candida albicans	1-deoxymannojirimycin
0.47	-	at pH 6.0 in 50 mM Tris-MES and at 37°C	Candida albicans	swainsonine

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Release 2023.1 (January 2023)