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Literature summary for 3.2.1.113 extracted from
- Heterologous expression and biochemical characterization of an alpha1,2-mannosidase encoded by the Candida albicans MNS1 gene (2008), Mem. Inst. Oswaldo Cruz, 103, 724-730 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 Star (DE3) cells | Candida albicans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-deoxymannojirimycin | - |
Candida albicans |  |
| swainsonine | - |
Candida albicans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
4-methylumbelliferyl alpha-D-mannopyranoside | at pH 6.0 and 37°C | Candida albicans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Candida albicans | 5783 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | Q8J0Q0 | - |
- |
| Candida albicans ATCC 26555 | Q8J0Q0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE Bio-Gel A column chromatography and Sephadex G-25 gel filtration | Candida albicans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0007 | - |
crude enzyme, at pH 6.0 and 37°C | Candida albicans |
| 0.1081 | - |
after 154fold purification, at pH 6.0 and 37°C | Candida albicans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl alpha-D-mannopyranoside + H2O | - |
Candida albicans | 4-methylumbelliferone + alpha-D-mannopyranose | - |
? | |
| 4-methylumbelliferyl alpha-D-mannopyranoside + H2O | - |
Candida albicans ATCC 26555 | 4-methylumbelliferone + alpha-D-mannopyranose | - |
? | |
| Man9GlcNAc2-Asn + H2O | - |
Candida albicans | Man8GlcNAc2-Asn (isomer 8A1,2,3B1,3) + D-mannose | - |
? | |
| Man9GlcNAc2-Asn + H2O | - |
Candida albicans ATCC 26555 | Man8GlcNAc2-Asn (isomer 8A1,2,3B1,3) + D-mannose | - |
? | |
| additional information | the enzyme fails to process Man5GlcNAc2-Asn | Candida albicans | ? | - |
? | |
| additional information | the enzyme fails to process Man5GlcNAc2-Asn | Candida albicans ATCC 26555 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, SDS-PAGE | Candida albicans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha1,2-mannosidase | - |
Candida albicans |
| MNS1 | - |
Candida albicans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Candida albicans |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.19 | - |
at pH 6.0 and 37°C | Candida albicans | 1-deoxymannojirimycin | |
| 0.62 | - |
at pH 6.0 and 37°C | Candida albicans | swainsonine | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is involved in the processing of N-linked mannans | Candida albicans |
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