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Literature summary for 3.2.1.113 extracted from

  • Tatara, Y.; Yoshida, T.; Ichishima, E.
    A single free cysteine residue and disulfide bond contribute to the thermostability of Aspergillus saitoi 1,2-alpha-mannosidase (2005), Biosci. Biotechnol. Biochem., 69, 2101-2108.
    View publication on PubMed
Search for more literature for 3.2.1.113

Protein Variants

Protein Variants Comment Organism
C334A Tm-value of mutant enzyme is 49.6°C compared to 55.8°C for the wild-type enzyme. Km-value is 2.4fold lower than wild-type value, turnover-number is 2.2fold lower than wild-type value Aspergillus phoenicis
C363A Tm-value of mutant enzyme is 49.6°C compared to 55.8°C for the wild-type enzyme. Km-value is 1.1fold higher than wild-type value, turnover-number is 1.1fold lower than wild-type value Aspergillus phoenicis
C443A Tm-value of mutant enzyme is 51.9°C compared to 55.8°C for the wild-type enzyme. Km-value is identical to wild-type value, turnover-number is 1.3fold lower than wild-type value Aspergillus phoenicis
C443D relative activity is less than 50% of wild-tzype activity Aspergillus phoenicis
C443G Tm-value of mutant enzyme is 50.2°C compared to 55.8°C for the wild-type enzyme Aspergillus phoenicis
C443L relative activity is less than 50% of wild-tzype activity Aspergillus phoenicis
C443M relative activity is less than 50% of wild-tzype activity Aspergillus phoenicis
C443S Tm-value of mutant enzyme is 50.0°C compared to 55.8°C for the wild-type enzyme Aspergillus phoenicis
C443T Tm-value of mutant enzyme is 52.8°C compared to 55.8°C for the wild-type enzyme Aspergillus phoenicis
C443V relative activity is less than 50% of wild-tzype activity Aspergillus phoenicis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.296
-
 Manalpha(1,2)Man-O-Me pH 5.0, 30°C, mutant enzyme C334A Aspergillus phoenicis
0.719
-
 Manalpha(1,2)Man-O-Me pH 5.0, 30°C, mutant enzyme C443A Aspergillus phoenicis
0.719
-
 Manalpha(1,2)Man-O-Me pH 5.0, 30°C, wild-type enzyme Aspergillus phoenicis
0.78
-
 Manalpha(1,2)Man-O-Me pH 5.0, 30°C, mutant enzyme C363A Aspergillus phoenicis

Organism

Organism UniProt Comment Textmining
Aspergillus phoenicis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-alpha-mannobiose + H2O
-
 Aspergillus phoenicis D-mannose
-
 ?
Manalpha(1,2)Man-O-Me + H2O
-
 Aspergillus phoenicis ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 Tm-value of mutant enzyme C443G, C334A, C363A and C443S Aspergillus phoenicis
52
-
 Tm-value of mutant enzyme C443A Aspergillus phoenicis
53
-
 Tm-value of mutant enzyme C443T Aspergillus phoenicis
56
-
 Tm-value of wild-type enzyme Aspergillus phoenicis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.87
-
 Manalpha(1,2)Man-O-Me pH 5.0, 30°C, mutant enzyme C334A Aspergillus phoenicis
6.67
-
 Manalpha(1,2)Man-O-Me pH 5.0, 30°C, mutant enzyme C443A Aspergillus phoenicis
8.51
-
 Manalpha(1,2)Man-O-Me pH 5.0, 30°C, wild-type enzyme Aspergillus phoenicis
9.48
-
 Manalpha(1,2)Man-O-Me pH 5.0, 30°C, mutant enzyme C363A Aspergillus phoenicis