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Literature summary for 3.2.1.113 extracted from

  • Liebminger, E.; Huettner, S.; Vavra, U.; Fischl, R.; Schoberer, J.; Grass, J.; Blaukopf, C.; Seifert, G.J.; Altmann, F.; Mach, L.; Strasser, R.
    Class I alpha-mannosidases are required for N-glycan processing and root development in Arabidopsis thaliana (2009), Plant Cell, 21, 3850-3867 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.113

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Nicotiana benthamiana leaf epidermal cells Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
1-deoxymannojirimycin
-
 Arabidopsis thaliana
EDTA
-
 Arabidopsis thaliana
kifunensine
-
 Arabidopsis thaliana
additional information not inhibitory: swainsonine; not inhibitory: swainsonine Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Arabidopsis thaliana 5794
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8H116
-
-
Arabidopsis thaliana Q8H116 isoform MNS2
-
Arabidopsis thaliana Q9C512
-
-
Arabidopsis thaliana Q9C512 isoform MNS1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)[Manalpha(1-2)Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Arabidopsis thaliana Manalpha(1-3)[Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)]Manbeta(1-4)GlcNACbeta(1-4)GlcNAc + 3 D-mannose
-
 ?
methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside
-
 Arabidopsis thaliana ? + D-mannose
-
 ?
[alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->2)-alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-N-Asn-[protein] + H2O i.e. isomer 8A1,2,3B1,3 Arabidopsis thaliana [alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-N-Asn-[protein] + D-mannose
-
 ?

Synonyms

Synonyms Comment Organism
At1g51590
-
 Arabidopsis thaliana
At3g21160
-
 Arabidopsis thaliana
MANIa
-
 Arabidopsis thaliana
MANIb
-
 Arabidopsis thaliana
MNS1
-
 Arabidopsis thaliana
MNS2
-
 Arabidopsis thaliana

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00035
-
 37°C, pH 6.0, substrate methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside Arabidopsis thaliana kifunensine
0.00047
-
 37°C, pH 6.0, substrate methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside Arabidopsis thaliana kifunensine
0.03
-
 37°C, pH 6.0, substrate methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside Arabidopsis thaliana 1-deoxymannojirimycin
0.04
-
 37°C, pH 6.0, substrate methyl 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranoside Arabidopsis thaliana 1-deoxymannojirimycin

General Information

General Information Comment Organism
physiological function in isoforms MNS1/MNS2 double mutant, MNS3 single mutant, accumulation of Man8GlcNAc2 is observed. A triple mutant lacking mannosidases MNS1, MNS2, and MNS3, EC 3.2.1.113, reveals the almost exclusive presence of Man9GlcNAc2 glycans. The MNS triple mutants display short, radially swollen roots and altered cell walls. Pharmacological inhibition of class I alpha-mannosidases in wild-type seedlings results in a similar root phenotype Arabidopsis thaliana
physiological function mutation results in Man(8)GlcNAc(2) accumulation in the Mns1 single mutant. In the Mns1/Mns2/Mns3 triple mutant, Man(9)GlcNAc(2) glycans are almost exclusively present. The Mns triple mutants display short, radially swollen roots and altered cell walls Arabidopsis thaliana
physiological function mutation results in Man(8)GlcNAc(2) accumulation in the Mns2 single mutant. In the Mns1/Mns2/Mns3 triple mutant, Man(9)GlcNAc(2) glycans are almost exclusively present. The Mns triple mutants display short, radially swollen roots and altered cell walls Arabidopsis thaliana