Information on EC 3.2.1.96 - mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.2.1.96
-
RECOMMENDED NAME
GeneOntology No.
mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -[Man(GlcNAc)2]Asn- structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Other glycan degradation
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-
SYSTEMATIC NAME
IUBMB Comments
glycopeptide-D-mannosyl-N4-(N-acetyl-D-glucosaminyl)2-asparagine 1,4-N-acetyl-beta-glucosaminohydrolase
A group of related enzymes.
CAS REGISTRY NUMBER
COMMENTARY hide
37278-88-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Arthrobacter protophormiae
Arthrobacter protophormiae RKJ100
-
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
strain C-125
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-
Manually annotated by BRENDA team
strain C-125
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain 168
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-
Manually annotated by BRENDA team
strain 168
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
no activity in bovine
cattle kidney
-
-
Manually annotated by BRENDA team
no activity in Enterococcus faecium
-
-
-
Manually annotated by BRENDA team
no activity in sheep
kidney
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain 22
-
-
Manually annotated by BRENDA team
strain 22
-
-
Manually annotated by BRENDA team
strain DW4
-
-
Manually annotated by BRENDA team
serotype M49
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
no activity detected in pig kidney
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
-
the enzyme is involved in the degradation mechanism of misfolded glycoproteins carrying exclusively high-mannose type N-glycans in the cytosol
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2S)-3-(benzyloxy)butane-1,2,4-triol + sialylglycopeptide
?
show the reaction diagram
-
transglycosylation
-
-
?
(GlcNAc)2(Man)5(GlcNAc)2Asn + H2O
?
show the reaction diagram
-
-
-
-
?
(GlcNAc)2(Man)6(GlcNAc)2 + H2O
GlcNAc + (GlcNAc)1(Man)6(GlcNAc)2
show the reaction diagram
-
-
-
?
(Man)3(GlcNAc)2-N-Asn-dansyl + H2O
?
show the reaction diagram
-
endo-beta-N-acetylglucosaminidase PII, no activity with endo-beta-N-acetylglucosaminidase PII
-
-
?
(Man)3(GlcNAc)2-N-dansyl-Asn + H2O
GlcNAc-N-dansyl-Asn + (Man)3-GlcNAc
show the reaction diagram
-
-
-
?
(Man)3(GlcNAc)2Asn + H2O
(Man)3GlcNAc + GlcNAc-Asn
show the reaction diagram
-
di-N-acetylchitobiosyl linkage is hydrolyzed faster than the linkage in the glycopeptides (Man)5(GlcNAc)3Asn and (Man)6(GlcNAc)2Asn by enzyme form F-I. No activity with the enzyme form F-II
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-
-
(Man)3(GlcNAc)2Asn-Ac + H2O
(Man)3GlcNAc + GlcNAc-Asn-Ac
show the reaction diagram
-
di-N-acetylchitobiosyl linkage is hydrolyzed faster than the linkage in the glycopeptides (Man)5(GlcNAc)3Asn and (Man)6(GlcNAc)2Asn by enzyme form F-I. No activity with the enzyme form F-II
-
-
?
(Man)3(Xyl)1(GlcNAc)2-pyridylamino + H2O
?
show the reaction diagram
(Man)5(GlcNAc)2-Asn + H2O
?
show the reaction diagram
-
-
-
?
(Man)5(GlcNAc)2-N-acetyl-Asn + H2O
(Man)5GlcNAc + GlcNAc-N-acetyl-Asn
show the reaction diagram
(Man)5(GlcNAc)2-N-dansyl-Asn + H2O
GlcNAc-N-dansyl-Asn + (Man)5-GlcNAc
show the reaction diagram
-
-
-
?
(Man)5(GlcNAc)2-pyridylamino + H2O
(Man)5(GlcNAc) + 2-(acetylamino)-2-deoxy-N-pyridin-2-yl-beta-D-glucopyranosylamine
show the reaction diagram
(Man)5(GlcNAc)2Asn + H2O
(Man)5GlcNAc + GlcNAc-Asn
show the reaction diagram
(Man)5(GlcNAc)2Asn-Ac + H2O
(Man)5GlcNAc + GlcNAc-Asn-Ac
show the reaction diagram
-
di-N-acetylchitobiosyl linkage is hydrolyzed faster than the linkage in the glycopeptides (Man)5(GlcNAc)3Asn and (Man)6(GlcNAc)2Asn by enzyme form F-I
-
-
?
(Man)5GlcNAc2-pyridylamino + H2O
?
show the reaction diagram
-
-
-
-
?
(Man)6(GlcNAc)2-Asn + D-glucose
(Man)6GlcNAcGlc + ?
show the reaction diagram
Arthrobacter protophormiae
-
-
-
?
(Man)6(GlcNAc)2-pyridylamino + H2O
?
show the reaction diagram
(Man)6(GlcNAc)2Asn + H2O
(Man)6GlcNAc + GlcNAc-Asn
show the reaction diagram
(Man)6(GlcNAc)2Asn-Ac + H2O
(Man)6GlcNAc + GlcNAc-Asn-Ac
show the reaction diagram
-
di-N-acetylchitobiosyl linkage is hydrolyzed faster than the linkage in the glycopeptides (Man)5(GlcNAc)3Asn and (Man)6(GlcNAc)2Asn by enzyme form F-I
-
-
?
(Man)6-GlcNAc-Glc-alpha-Glc + H2O
?
show the reaction diagram
Arthrobacter protophormiae
-
-
-
-
?
(Man)6GlcNAc2-pyridylamino + H2O
?
show the reaction diagram
-
-
-
-
?
(Man)7(GlcNAc)2-pyridylamino + H2O
?
show the reaction diagram
(Man)8(GlcNAc)2-pyridylamino + H2O
?
show the reaction diagram
(Man)9(GlcNAc)2-pyridylamino + H2O
?
show the reaction diagram
(Man)9GlcNAc2-pyridylamino + H2O
?
show the reaction diagram
-
-
-
-
?
1,3-di-O-benzyl-D-erythritol + sialylglycopeptide
?
show the reaction diagram
-
transglycosylation
-
-
?
2-methyl-[alpha-D-mannopyranosyl-(1,3)-beta-D-mannopyranosyl-(1,4)-alpha-D-glucopyrano]-[2,1-d]-oxazoline + N4-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-1-methyl-N2-(benzyloxycarbonyl)-L-asparagine
(N4-(alpha-D-mannopyranosyl)-(1,3)-beta-D-mannopyranosyl-(1,4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamido-2-deoxy-beta-D-glucopyranosyl-1-methyl-N2-benzyloxycarbonyl)-L-asparagine + ?
show the reaction diagram
-
-
yield 81%
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-
4-methylumbelliferyl di-N-acetyl-beta-chitobioside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl N,N',N''-triacetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N,N',N''-triacetyl-beta-D-glucosaminide
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
4-methylumbelliferol + alpha-D-mannopyranose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-mannopyranoside + H2O
4-methylumbelliferone + alpha-D-mannopyranose
show the reaction diagram
4-methylumbelliferyl-beta-D-mannopyranoside + H2O
4-methylumbelliferone + beta-D-mannopyranose
show the reaction diagram
4-methylumbelliferyl-beta-D-N-acetylglucosaminide + H2O
4-methylumbelliferone + beta-D-N-acetylglucosamine
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-mannopyranoside + H2O
4-methylumbelliferol + beta-D-mannopyranose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl-beta-D-N-acetylglucosamine + H2O
4-nitrophenol + N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
-
?
4-nitrophenyl-GlcNAc + Lys-Val-Ala-Asn[(NeuAc-Gal-GlcNAc-Man)2-ManGlcNAc2]-Lys-Thr
?
show the reaction diagram
-
-
-
-
?
9-fluorenylmethyloxycarbonyl-labeled asparagine-linked agalactobiantennary oligosaccharide + 4-nitrophenyl-beta-D-glucopyranoside
?
show the reaction diagram
transglycosylation activity
-
-
?
9-fluorenylmethyloxycarbonyl-labeled asparagine-linked agalactobiantennary oligosaccharide + H2O
9-fluorenylmethyloxycarbonyl-labeled asparagine-linked N-acetylglucosamine (GlcNAc) + ?
show the reaction diagram
-
-
-
?
9H-fluoren-9-ylmethyl [(3R)-1,3-dihydroxybutan-2-yl]carbamate + H2O
?
show the reaction diagram
-
transglycosylation
-
-
?
acetyl-Asn(GlcNAc)2(Man)6 + H2O
acetyl-AsnGlcNAc
show the reaction diagram
alpha1-acid glycoprotein + H2O
?
show the reaction diagram
-
-
-
-
?
asialo-N-acetyl-lactosaminic-type glycoasparagine + H2O
GlcNAc-Asn + ?
show the reaction diagram
-
-
-
?
Asn(GlcNAc)2(Man)3 + H2O
?
show the reaction diagram
Asn(GlcNAc)2(Man)3(Fuc)1 + H2O
?
show the reaction diagram
-
-
-
-
?
Asn(GlcNAc)2-(Man)5 + H2O
AsnGlcNAc + Man5(GlcNAc)
show the reaction diagram
Asn(GlcNAc)2-(Man)6 + H2O
AsnGlcNAc + (GlcNAc)1-(Man)6
show the reaction diagram
Asn(GlcNAc)2-(Man)6(GlcNAc)2 + H2O
AsnGlcNAc + (GlcNAC)2(Man)6(GlcNAc)2
show the reaction diagram
Asn(GlcNAc)4(Man)6 + H2O
?
show the reaction diagram
-
-
-
-
?
Asn(Man4GlcNAc4) + H2O
?
show the reaction diagram
-
-
-
?
Asn-(GlcNAc)2(Man)6 + gentiobiose
?
show the reaction diagram
Asn-(GlcNAc)2(Man)6 + glucose
?
show the reaction diagram
Asn-Tyr-Asn(GlcNAc)2(Man)3(GlcNAc)2-(Gal)2-Lys + H2O
Asn-Tyr-Asn(GlcNAc)Lys + (Gal)2(GlcNAc)2(Man)3(GlcNAc)
show the reaction diagram
-
-
-
?
beta-Glcp-(1-4)-GlcpNAc-oxazoline
?
show the reaction diagram
Arthrobacter protophormiae
-
Endo-A shows only marginal activity for transglycosylation with the disaccharide oxazoline. When used in a relatively large quantity, Endo-A can promote the transglycosylation of the disaccharide oxazoline to a GlcpNAc-Asn acceptor (it catalyzes the transfer of alpha-Manp-(1-3)-beta-Glcp-(1-4)-GlcpNAc-oxazoline to the acceptor). Endo-A promotes polymerization of beta-Glcp-(1-4)-GlcpNAc-oxazoline
-
-
?
calf fetuin + 4-nitrophenyl-beta-D-glucoside
?
show the reaction diagram
-
-
transfer of triantennary complex type oligosaccharide, yield 3.4%
-
?
carboxypeptidase Y + Lys-Val-Ala-Asn((NeuAc-Gal-GlcNAc-Man)2Man-GlcNAc2)-Lys-Thr
?
show the reaction diagram
-
-
change of hyperglycosylated protein to a sialyl complex type glycoprotein
-
-
complex-type aspargine-linked oligosaccharide + H2O
?
show the reaction diagram
-
hydrolysis of the di-N-acetylchitobiose structure. The specificity for complex type oligosaccharides is probably defined by multiple domains in Endo D structure
-
-
?
dansyl-Asn-(GlcNAc)2(Man)5 + H2O
dansyl-Asn-GlcNAc + ?
show the reaction diagram
Arthrobacter protophormiae
-
-
-
-
?
dansyl-Asn-(GlcNAc)2(Man)6 + H2O
dansyl-Asn-GlcNAc + (Man)6GlcNAc
show the reaction diagram
dansyl-Asn-(GlcNAc)2(Man)6(GlcNAc)2 + H2O
?
show the reaction diagram
-
-
-
-
?
dansyl-Asn-(GlcNAc)4(Man)6 + H2O
dansyl-Asn-GlcNAc + ?
show the reaction diagram
dansyl-Lys-Val-Ala-Asn((NeuAc-Gal-GlcNAc-Man)2Man-GlcNAc2)-Lys-Thr + H2O
?
show the reaction diagram
-
-
-
-
?
denatured RNase B + H2O
?
show the reaction diagram
-
-
-
-
?
dolichyl pyrophosphoryl oligosaccharides + H2O
?
show the reaction diagram
-
both glucosylated and unglucosylated polymannose oligosaccharides are released by the enzyme through cleavage of the di-N-acetylchitobiose squence
-
-
?
fetuin + H2O
?
show the reaction diagram
-
-
-
-
?
fluoresceinyl 2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
fluorescein + ?
show the reaction diagram
-
-
-
?
fluoresceinyl di-N-acetyl-beta-chitobioside + H2O
fluorescein + ?
show the reaction diagram
-
-
-
?
GlcNAc-Asn + Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAc
Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn
show the reaction diagram
Arthrobacter protophormiae
Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAc-thiazoline is a transition state mimic and is resistant to Endo-A hydrolysis
-
-
?
GlcNAc1(Man)5(GlcNAc)2-pyridylamino
?
show the reaction diagram
GlcNAcAsn + H2O
N-acetyl-D-glucosamine + L-asparagine
show the reaction diagram
glycopeptide + H2O
?
show the reaction diagram
glycoprotein + H2O
?
show the reaction diagram
glycosylated ascorbate oxidase + H2O
?
show the reaction diagram
-
-
-
-
?
glycosylated human apo-transferrin + H2O
?
show the reaction diagram
-
-
-
-
?
glycosylated mAb 2G12 + H2O
?
show the reaction diagram
-
-
-
-
?
glycosylated type VI-A horseraddish peroxidase + H2O
?
show the reaction diagram
-
-
-
-
?
H-Lys-Val-Ala-Asn[(NeuAc-Gal-GlcNAc-Man)2-Man-GlcNAc2]-Lys-Thr-OH + (1S)-1,5-anhydro-1-(2,4,6-trimethoxyphenyl)-D-glucitol
?
show the reaction diagram
-
-
-
-
?
H-Lys-Val-Ala-Asn[(NeuAc-Gal-GlcNAc-Man)2-Man-GlcNAc2]-Lys-Thr-OH + 1-deoxy-1-phenyl-alpha-D-gluco-2-heptulopyranose
?
show the reaction diagram
-
-
-
-
?
H-Lys-Val-Ala-Asn[(NeuAc-Gal-GlcNAc-Man)2-Man-GlcNAc2]-Lys-Thr-OH + 2-benzamido-2-deoxy-D-glucopyranose
?
show the reaction diagram
-
-
-
-
?
H-Lys-Val-Ala-Asn[(NeuAc-Gal-GlcNAc-Man)2-Man-GlcNAc2]-Lys-Thr-OH + 2-benzoylcarbonylamino-2-deoxy-D-glucopyranose
?
show the reaction diagram
-
-
-
-
?
H-Lys-Val-Ala-Asn[(NeuAc-Gal-GlcNAc-Man)2-Man-GlcNAc2]-Lys-Thr-OH + 2-deoxy-2-naphthoamido-D-glucopyranose
?
show the reaction diagram
-
-
-
-
?
H-Lys-Val-Ala-Asn[(NeuAc-Gal-GlcNAc-Man)2-Man-GlcNAc2]-Lys-Thr-OH + 2-deoxy-2-phenoxyacetamido-D-glucopyranose
?
show the reaction diagram
-
-
-
-
?
H-Lys-Val-Ala-Asn[(NeuAc-Gal-GlcNAc-Man)2-Man-GlcNAc2]-Lys-Thr-OH + 2-deoxy-2-phthalimido-D-glucopyranose
?
show the reaction diagram
-
-
-
-
?
H-Lys-Val-Ala-Asn[(NeuAc-Gal-GlcNAc-Man)2-Man-GlcNAc2]-Lys-Thr-OH + benzyl beta-D-glucopyranoside
?
show the reaction diagram
-
-
-
-
?
haptoglobin + H2O
?
show the reaction diagram
-
-
-
-
?
hen egg glycopeptide + 4-nitrophenyl-beta-D-glucose
?
show the reaction diagram
-
-
transfer of asparagine-linked oligosaccharide
-
?
human alpha1-acid glycoprotein + 4-nitrophenyl-beta-D-glucose
?
show the reaction diagram
-
-
transfer of bi-, tri- and tetraantennary complex type oligosaccharide, yield 1.7%
-
?
invertase + Lys-Val-Ala-Asn((NeuAc-Gal-GlcNAc-Man)2Man-GlcNAc2)-Lys-Thr
?
show the reaction diagram
-
-
change of hyperglycosylated protein to a sialyl complex type glycoprotein
-
-
keratan sulfate + H2O
?
show the reaction diagram
hydrolyzes keratan sulfate between the 4GlcNAcbeta1-3Gal1 structure, digests shark cartilage keratan sulfate to disaccharides and tetrasaccharides and bovine cornea keratan sulfate to hexasaccharides, prefers highly sulfated keratan sulfate
-
-
?
lectin + H2O
?
show the reaction diagram
-
-
-
-
?
Lys-Val-Ala-Asn((NeuAc-Gal-GlcNAc-Man)2Man-GlcNAc2)-Lys-Thr + 4-methylumbelliferyl-beta-D-N-acetylglucosaminide
?
show the reaction diagram
-
-
-
-
-
Man(GlcNAc)2Asn + H2O
ManGlcNAc + GlcNAcAsn
show the reaction diagram
-
-
-
-
?
Man(GlcNAc)2Asn-dansyl + H2O
ManGlcNAc + GlcNAcAsn-dansyl
show the reaction diagram
-
-
-
-
?
Man3GlcNAc-oxazoline + Fmoc-Asn(Fucalpha(1->6)GlcNAc)-OH
?
show the reaction diagram
-
-
-
-
?
Man3GlcNAc-oxazoline + Fmoc-Asn(GlcNAc)-OH
?
show the reaction diagram
-
-
-
-
?
Manalpha1-2Manalpha1-6(Manalpha1-2Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
Manalpha1-2Manalpha1-6(Manalpha1-2Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAc + 2-(acetylamino)-2-deoxy-N-pyridin-2-yl-beta-D-glucopyranosylamine
show the reaction diagram
-
-
-
-
?
Manalpha1-2Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
Manalpha1-2Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAc + 2-(acetylamino)-2-deoxy-N-pyridin-2-yl-beta-D-glucopyranosylamine
show the reaction diagram
-
-
-
-
?
Manalpha1-2Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
?
show the reaction diagram
-
-
-
-
?
Manalpha1-3Manalpha1-6(Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
?
show the reaction diagram
-
-
-
-
?
Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAc + 2-(acetylamino)-2-deoxy-N-pyridin-2-yl-beta-D-glucopyranosylamine
show the reaction diagram
-
-
-
-
?
Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
?
show the reaction diagram
-
-
-
-
?
Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-pyridylamino + H2O
?
show the reaction diagram
-
-
-
-
?
Manalpha1-6(Manalpha1-3)Manalpha1-6(Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc-PA + H2O
?
show the reaction diagram
-
-
-
-
?
Manalpha1->6(Manalpha1->3)Manalpha1->6(Manalpha1->2Manalpha1->3)Manbeta1->4GlcNAcbeta1->4GlcNAcAsn + H2O
GlcNAc->N-acetylAsn + ?
show the reaction diagram
Manalpha1->6(Manalpha1->3)Manbeta1->4GlcNAcbeta1->4(Fucalpha1->6)GlcNAc->N-acetylAsn + H2O
Manalpha1->6(Manalpha1->3)Manbeta1->4GlcNAc + Fucalpha1-6GlcNAc-N-acetylAsn
show the reaction diagram
N,N',N'',N'''-tetraacetylchitotetraose + H2O
2 N,N'-diacetylchitobiose
show the reaction diagram
-
-
-
?
N,N',N''-triacetylchitotriose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchiotobiose
show the reaction diagram
-
-
-
?
N-glycosylated bovine lactoferrin + H2O
?
show the reaction diagram
N-glycosylated bovine serum albumin + H2O
?
show the reaction diagram
N-glycosylated human lactoferrin + H2O
?
show the reaction diagram
N-glycosylated human serum + H2O
Man5GlcNAc + ?
show the reaction diagram
-
-
main product
-
?
N-glycosylated immunglobuline G + H2O
?
show the reaction diagram
N-glycosylated immunoglobulin A + H2O
?
show the reaction diagram
N-glycosylated immunoglobulin G + H2O
?
show the reaction diagram
N-glycosylated lactoferrin + H2O
?
show the reaction diagram
N-glycosylated orosomucoid + H2O
?
show the reaction diagram
the enzyme cleaves only biantennary and sialylated structures of orosomucoid
-
-
?
N-glycosylated ovalbumin + H2O
?
show the reaction diagram
-
-
-
-
?
N-glycosylated RNase B + H2O
?
show the reaction diagram
N-glycosylated RNase B + H2O
Man5GlcNAc + ?
show the reaction diagram
-
-
main product
-
?
N-glycosylated silkworm hemolymph glycoproteins + H2O
?
show the reaction diagram
-
-
-
-
?
N-glycosylated storage protein 2 + H2O
?
show the reaction diagram
-
-
-
-
?
N-glycosylated transferrin + H2O
?
show the reaction diagram
-
-
-
?
N-glycosylated yeast invertase + H2O
?
show the reaction diagram
-
-
-
-
?
N4-(2-acetamido-3-O-benzyl-2-deoxy-beta-D-glucopyranosyl)-N2-(9-fluorenylmethoxycarbonyl)asparagine + H2O
?
show the reaction diagram
N4-(2-acetamido-4-O-benzyl-2-deoxy-beta-D-glucopyranosyl)-N2-(9-fluorenylmethoxycarbonyl)-L-asparagine + H2O
?
show the reaction diagram
N4-(2-acetamido-6-O-benzyl-2-deoxy-beta-D-glucopyranosyl)-N2-(9-fluorenylmethoxycarbonyl)-L-asparagine + H2O
?
show the reaction diagram
N4-(alpha-L-fucopyranosyl-(1 ->3)-2-acetamido-2-deoxy-beta-D-glucopyranosyl)-N2-(9-fluorenylmethoxycarbonyl)-L-asparagine + H2O
?
show the reaction diagram
Nalpha-9-fluorenylmethoxycarbonyl-Nbeta-3-hydroxymetyl-4-hydroxy-cyclohexyl-L-asparagine + sialylglycopeptide
?
show the reaction diagram
-
transglycosylation
-
-
?
O-(alpha-D-mannopyranosyl)-(1,6)-[(alpha-D-mannopyranosyl)-(1,3)]-beta-D-mannopyranosyl-(1,4)-(1,2-dideoxy-alpha-D-glucopyrano)-[2,1-d]-2-oxazoline + benzyl alpha-D-mannopyranoside
2,3,4,6-tetra-O-acetyl-1-O-(2,2,2-trichloroethanimidoyl)-alpha-D-mannopyranose + ?
show the reaction diagram
Arthrobacter protophormiae
-
-
-
-
?
O-(beta-D-mannopyranosyl)-(1,4)-(1,2-dideoxy-alpha-D-glucopyrano)-[2,1-d]-2-oxazoline + 2-O-acetyl-3,4,6-tri-O-benzyl-alpha-D-mannopyranosyl trichloroacetimidate
ethyl 2-O-acetyl-3,4,6-tri-O-benzyl-alpha-D-mannopyranosyl-(1-3)-4,6-O-benzylidene-2-O-levulinoyl-1-thio-beta-D-glucopyranoside + ?
show the reaction diagram
-
-
yield of 98%
-
-
O-(beta-D-mannopyranosyl)-(1,4)-(1,2-dideoxy-alpha-D-glucopyrano)-[2,1-d]-2-oxazoline + benzyl alpha-D-mannopyranoside
benzyl 2,4-di-O-benzoyl-alpha-D-mannopyranoside + ?
show the reaction diagram
Arthrobacter protophormiae
-
-
-
-
?
ovalbumin + H2O
oligosaccharides + ?
show the reaction diagram
peptidoglycan + H2O
?
show the reaction diagram
ribonuclease B + H2O
?
show the reaction diagram
Ricinus lectin + H2O
?
show the reaction diagram
-
-
-
-
-
RNase B + H2O
?
show the reaction diagram
RNase B + Lys-Val-Ala-Asn((NeuAc-Gal-GlcNAc-Man)2Man-GlcNAc2)-Lys-Thr
?
show the reaction diagram
-
-
change of high-mannose type oligosaccharide of Rnase B to a sialyl complex type
-
-
transferrin + 4-nitrophenyl-alpha-D-galactoside
?
show the reaction diagram
-
-
transfer of asialobiantennary complex type oligosaccharide of human native transferrin
-
?
transferrin + 4-nitrophenyl-alpha-D-glucoside
?
show the reaction diagram
-
-
transfer of asialobiantennary complex type oligosaccharide of human native transferrin
-
?
transferrin + 4-nitrophenyl-beta-D-galactoside
?
show the reaction diagram
-
-
transfer of asialobiantennary complex type oligosaccharide of human native transferrin
-
?
transferrin + 4-nitrophenyl-beta-D-glucoside
?
show the reaction diagram
-
-
transfer of asialobiantennary complex type oligosaccharide of human native transferrin with yield of 1.1%
-
?
transferrin + 4-nitrophenyl-beta-D-mannoside
?
show the reaction diagram
-
-
transfer of asialobiantennary complex type oligosaccharide of human native transferrin
-
?
transferrin + 4-nitrophenyl-beta-D-xyloside
?
show the reaction diagram
-
-
transfer of asialobiantennary complex type oligosaccharide of human native transferrin
-
?
transferrin + 4-nitrophenyl-glycerol
?
show the reaction diagram
-
-
transfer of asialobiantennary complex type oligosaccharide of human native transferrin
-
?
transferrin + 4-nitrophenyl-N-acetyl-beta-D-glucosaminide
?
show the reaction diagram
-
-
transfer of asialobiantennary complex type oligosaccharide of human native transferrin
-
?
transferrin + H2O
complex oligosaccharides + ?
show the reaction diagram
-
-
-
?
yeast carboxypeptidase + H2O
?
show the reaction diagram
-
-
-
-
?
yeast invertase + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-glycosylated immunoglobulin G + H2O
?
show the reaction diagram
N-glycosylated lactoferrin + H2O
?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Li+
-
maximal activation near 0.2 M LiCl with Micrococcus luteus cell walls as substrate, sharp optimal activation at 0.1 M with Bacillus subtilis cell walls as substrate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetate
-
endo-beta-N-acetylglucosaminidase 2
Ag2+
-
1 mM, significant inactivation
alpha-D-mannosides
-
-
choline
-
2%, activates
Citric acid
-
-
Co2+
-
10 mM, 75% inhibition
galactose
-
0.5 M, 23% inhibition
glycoprotein
-
glycoproteins present in the rat liver homogenate
lipoteichoic acid
Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAc-thiazoline
Arthrobacter protophormiae
-
-
mannose
methyl alpha-D-mannoside
N-acetylglucosamino(1->5)lactone
-
-
O-(2,3,4,6-tetra-O-acetyl-alpha-D-mannopyranosyl)-(1,6)-[(2,3,4,6-tetra-O-acetyl-alpha-D-mannopyranosyl)-(1,3)]-2,4-di-O-acetyl-beta-D-mannopyranosyl-(1,4)-(3,6-di-O-acetyl-1,2-dideoxy-alpha-D-glucopyrano)-[2,1-d]-2-thiazoline
O-(alpha-D-mannopyranosyl)-(1,6)-[(alpha-D-mannopyranosyl)-(1,3)]-beta-D-mannopyranosyl-(1,4)-(1,2-dideoxy-alpha-D-glucopyrano)-[2,1-d]-2-thiazoline
O-(beta-D-mannopyranosyl)-(1,4)-(1,2-dideoxy-alpha-D-glucopyrano)-[2,1-d]-2-thiazoline
p-Chloromercuriphenyl sulfonate
-
-
p-nitrophenyl alpha-D-mannoside
-
0.01 M, 70% inhibition; 0.01 M, no inhibition
yeast mannan
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
ovalbumin, fetuin, bromelain, ovomucoid, alpha1-acid glycoprotein, immunoglobulin G and influenza virus hemagglutinin are susceptible only after reduction and alkylation or in presence of 1% 2-mercaptoethanol
choline
-
0.1%, activates
dithiothreitol
L-Cys
-
1 mM, 18% stimulation
nisin
-
activates hydrolysis of cell walls of Micrococcus luteus, no influence of hydrolysis of soluble peptidoglycan
Nonionic detergent
-
glycoproteins of retrovirus, lymphocytic choriomeningitis virus; Pichinde virus, and HLA-A and HLA-B antigens are cleaved in presence of nonionic detergent
-
Pep 5
-
cationic peptide antibiotic, activates hydrolysis of cell walls of Micrococcus luteus, no influence of hydrolysis of soluble peptidoglycan
-
poly(L-Lys)
-
activates hydrolysis of cell walls of Micrococcus luteus, no influence of hydrolysis of soluble peptidoglycan
Triton X-100
-
maximal stimulation at 0.03%, or small amounts of other detergents
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4
(Man)5(GlcNAc)2-pyridylamino
-
-
0.2
(Man)5(GlcNAc)2Asn-acetyl
-
-
0.025
(Man)6(GlcNAc)2-pyridylamidated
-
-
0.25
(Man)6(GlcNAc)2-pyridylamino
-
-
0.32
(Man)9(GlcNAc)2-pyridylamino
-
-
0.00625
4-methylumbelliferyl di-N-acetyl-beta-chitobioside
-
-
-
0.0204
4-methylumbelliferyl N,N',N''-triacetyl-beta-D-glucosaminide
-
at pH 5.0 and 37C
-
3.5 - 77.4
4-nitrophenyl-beta-D-N-acetylglucosamine
5.54
9-fluorenylmethyloxycarbonyl-labeled asparagine-linked agalactobiantennary oligosaccharide
at pH 5.5 and 50C
-
0.25
Asn(GlcNAc)2-(Man)5
-
-
-
0.68 - 2
dansyl asialotransferrin glycopeptide
0.0868
dansyl derivative of Taka-amylase A glycopeptide
-
-
-
0.207 - 0.876
dansyl-Asn-(GlcNAc)2(Man)5
0.3
dansyl-Asn-(GlcNAc)2(Man)6
8.51 - 18.51
Fmoc-Asn(Fucalpha(1->6)GlcNAc)-OH
0.73 - 5.22
Fmoc-Asn(GlcNAc)-OH
54 - 143
Keratan sulfate
0.7
Man(GlcNAc)2Asn-dansyl
-
-
0.5 - 0.67
Man3GlcNAc-oxazoline
0.05
Manalpha1->6(Manalpha1->3)Manalpha1->6(Manalpha1->2Manalpha1->3)Manbeta1->4GlcNAcbeta1->4GlcNAcAsn
-
endo-beta-N-acetylglucosaminidase CII
0.032
Manalpha1->6(Manalpha1->3)Manbeta1->4GlcNAcbeta1->4(Fucalpha1->6)GlcNAc->N-acetylAsn
-
endo-beta-N-acetylglucosaminidase CI
2.56
ovalbumin
-
-
-
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11 - 250
4-nitrophenyl-beta-D-N-acetylglucosamine
5.91
9-fluorenylmethyloxycarbonyl-labeled asparagine-linked agalactobiantennary oligosaccharide
Ogataea minuta
R4WHQ8
at pH 5.5 and 50C
-
0.0458 - 365
dansyl-Asn-(GlcNAc)2(Man)5
0.24 - 14.32
Fmoc-Asn(Fucalpha(1->6)GlcNAc)-OH
0.5 - 6.37
Fmoc-Asn(GlcNAc)-OH
0.21 - 10.17
Man3GlcNAc-oxazoline
additional information
additional information
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.023 - 1.28
4-nitrophenyl-beta-D-N-acetylglucosamine
0.0286 - 0.77
Fmoc-Asn(Fucalpha(1->6)GlcNAc)-OH
0.096 - 8.7
Fmoc-Asn(GlcNAc)-OH
0.31 - 20.4
Man3GlcNAc-oxazoline
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2000
Urea
-
-
10
Zn2+
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00022 - 0.006
O-(2,3,4,6-tetra-O-acetyl-alpha-D-mannopyranosyl)-(1,6)-[(2,3,4,6-tetra-O-acetyl-alpha-D-mannopyranosyl)-(1,3)]-2,4-di-O-acetyl-beta-D-mannopyranosyl-(1,4)-(3,6-di-O-acetyl-1,2-dideoxy-alpha-D-glucopyrano)-[2,1-d]-2-thiazoline
0.013 - 0.064
O-(alpha-D-mannopyranosyl)-(1,6)-[(alpha-D-mannopyranosyl)-(1,3)]-beta-D-mannopyranosyl-(1,4)-(1,2-dideoxy-alpha-D-glucopyrano)-[2,1-d]-2-thiazoline
0.038 - 0.08
O-(beta-D-mannopyranosyl)-(1,4)-(1,2-dideoxy-alpha-D-glucopyrano)-[2,1-d]-2-thiazoline
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00039
-
mutant N175A, hydrolytic activity, pH 6.25, 30C
0.0017
-
mutant N249S, transglycosylation activity, pH 6.25, 30C
0.0018
-
mutant W251N, transglycosylation activity, pH 6.25, 30C
0.0027
-
mutant Y250N, transglycosylation activity, pH 6.25, 30C
0.01
Arthrobacter protophormiae
-
specific transglycosylation activity of mutant N171A
0.023
-
mutant N230S, transglycosylation activity, pH 6.25, 30C; mutant Y217F/W228F, transglycosylation activity, pH 6.25, 30C
0.026
-
mutant W228F, transglycosylation activity, pH 6.25, 30C
0.058
-
wild-type, transglycosylation activity, pH 6.25, 30C
0.085
-
mutant Y217F, transglycosylation activity, pH 6.25, 30C
0.299
-
endo-beta-N-acetylglucosaminidase CI
0.3
-
mutant Y250N, hydrolytic activity, pH 6.25, 30C
0.304
-
endo-beta-N-acetylglucosaminidase CII
0.32
-
mutant N249S, hydrolytic activity, pH 6.25, 30C
0.43
-
mutant W251N, hydrolytic activity, pH 6.25, 30C
0.46
-
mutant W228F, hydrolytic activity, pH 6.25, 30C
0.62
-
mutant Y217F/W228F, hydrolytic activity, pH 6.25, 30C
0.7
-
mutant N230S, hydrolytic activity, pH 6.25, 30C
1.2
-
mutant Y217F, hydrolytic activity, pH 6.25, 30C
1.6
-
wild-type, hydrolytic activity, pH 6.25, 30C
1.61
Arthrobacter protophormiae
-
specific transglycosylation activity of mutant Y205F
1.72
Arthrobacter protophormiae
-
specific transglycosylation activity of the wild-type
5.11
Arthrobacter protophormiae
-
specific transglycosylation activity of mutant Y299F
9.5
-
purified recombinant wild-type enzyme, hydrolysis of natural N-glycan substrate
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 3.5
4 - 4.8
-
hydrolysis of dansyl derivative of Taka-amylase A glycopeptide
4 - 4.5
4.5 - 5.5
-
Micrococcus luteus cell walls as substrate, in 50 mM acetic acid/acetate buffer or succinate/NaOH buffer
4.5
-
Bacillus subtilis cell walls
4.5
-
endo-beta-N-acetylglucosaminidase 1
5 - 11
Arthrobacter protophormiae
-
-
5 - 9
Arthrobacter protophormiae
-
-
5 - 9
-
enzyme is most active within this range
5
-
hydrolysis of ovalbumin
5.4
-
hydrolysis of (Man)5(GlcNAc)2Asn-acetyl, enzyme type F-II
5.5 - 6
-
enzyme form endo-BI and endo-BII
5.9
-
hydrolysis of (Man)5(GlcNAc)2Asn-acetyl, enzyme type F-I
6 - 6.5
-
ENGase CI
6 - 6.5
-
-
6 - 8
-
ENGase PII
6
-
hydrolysis of (Man)6(GlcNAc)2-pyridylamino
7.2
-
transglycosylation assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.3 - 5.5
-
pH 2.3: about 20% of maximal activity, pH 5.5: about 25% of maximal activity
2.5 - 5
-
about 30% of maxiaml activity at pH 2.5 and at pH 5.0
2.5 - 8
-
pH 2.5: about 20% of maximal activity, pH 8.0: about 30% of maximal activity
2.5 - 4.5
-
about 70% of maximal activity at pH 2.5 and at pH 4.5, endo-beta-N-acetylglucosaminidase 2
3 - 8
-
pH 3.0-5.0: optimum, pH 8.0: 60% of maximal activity
3.3 - 6.3
-
pH 3.3: about 60% of maximal activity, pH 6.3: about 50% of maximal activity
4 - 7
-
the enzymatic activity of the recombinant enzyme is retained at pH 4.0-7.0
4.5 - 8
-
pH 4.5: about 75% of maximal activity, pH 8.0: about 40% of maximal activity
4.5 - 8.5
-
pH 4.5: about 25% of maximal activity, pH 8.5: about 30% of maximal activity
4.5 - 7
-
pH 4.5: about 60% of maximal activity, pH 7.0: about 50% of maximal activity, endo-beta-N-acetylglucosaminidase 1
4.5 - 8
the activity is decreased less than 20% at pH 4.5 or 8.0
5 - 8.5
-
about 55% of maximal activity at pH 5.0 and at pH 8.5
5.3 - 8.2
-
about 50% of maximal activity at pH 5.3 and at pH 8.2
5.5 - 9.5
-
pH 5.5: maximal activity, pH 9.5: about 40% of maximal activity, hydrolysis of dansyl-Asn(GlcNAc)2(Man)6
6 - 7.5
-
80% of maximal activity at pH 6.0 and at pH 7.5
9
-
60% activity at pH 9
10
-
28% activity at pH 10
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27 - 47
-
the enzymatic activity of the recombinant enzyme is retained at 27-47C
30 - 45
30
-
assay at
45 - 50
-
-
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 60
-
25C: about 80% of maximal activity, 60C: about 45% of maximal activity
50 - 60
80% activity at 50C, 79% activity at 60C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
high activity
Manually annotated by BRENDA team
; weak expression
Manually annotated by BRENDA team
-
highest activity in rootlets
Manually annotated by BRENDA team
-
epithelial cells peeling from the oral cavity epithelium into saliva
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
recombinant enzyme produced in Escherichia coli appears both in the periplasmic space and in the cell
Manually annotated by BRENDA team
-
no evidence for a lysosomal enzyme form
Manually annotated by BRENDA team
Arthrobacter protophormiae
-
recombinant enzyme
-
Manually annotated by BRENDA team
-
recombinant enzyme produced in Escherichia coli appears both in the periplasmic space and in the cell
Manually annotated by BRENDA team
-
associated with
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Arthrobacter protophormiae
Arthrobacter protophormiae
Arthrobacter protophormiae
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
Staphylococcus aureus (strain NCTC 8325)
Staphylococcus aureus (strain NCTC 8325)
Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Streptococcus pyogenes serotype M1 (strain ATCC BAA-947 / MGAS5005)
Streptococcus pyogenes serotype M1 (strain ATCC BAA-947 / MGAS5005)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
17500
-
enzyme type F-II, gel filtration
23500
-
gel filtration
27200
-
equilibrium sedimentation
30000
-
SDS-PAGE
31000
-
gel filtration
35000
-
gel filtration
41670
-
MALDI-TOF mass spectrometry
46000
-
gel filtration
49500
-
equilibrium sedimentation
52000
-
enzyme type F-I, gel filtration
54000
-
gel filtration
55000
SDS-PAGE
56000
-
gel filtration
90000
-
SDS-PAGE
95000
-
gel filtration
150000
-
enzyme form endo-BII, non-denaturing PAGE
178000
-
SDS-PAGE
190000
-
gel filtration
200000
SDS-PAGE and calculated from amino acid sequence
210000
-
enzyme form endo-BI, non-denaturing PAGE
280000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer