Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 1 mM, 18% stimulation | Aspergillus oryzae | |
L-Cys | 1 mM, 18% stimulation | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0868 | - |
dansyl derivative of Taka-amylase A glycopeptide | - |
Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
gel filtration | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Streptomyces griseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus oryzae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Aspergillus oryzae |
Storage Stability | Organism |
---|---|
stable for at least 4 months in the refrigerator at pH 4.0-6.0 | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dansyl derivative of Taka-amylase A glycopeptide + H2O | - |
Aspergillus oryzae | ? | - |
? | |
additional information | - |
Streptomyces griseus | ? | - |
? | |
additional information | high-mannose-type glycopeptides are rapidly hydrolyzed, hybrid-type glycopeptides are good substrates, complex-type glycopeptides as well as smaller glycopeptides prepared by partial glycosidase digestion of complex-type glycopeptides are resistant to the enzyme | Aspergillus oryzae | ? | - |
? | |
Taka-amylase A glycopeptide + H2O | dansyl derivative of taka-amylase A glycopeptide | Aspergillus oryzae | additional information | product contains N-acetylglucosaminitol and mannose in the molar ratio 1.0:5.2, but no N-acetylglucosamine or mannitol | ? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 5.0, 0.1 M acetate buffer, 2 h, less than 10% loss of activity | Aspergillus oryzae |
60 | - |
10 min, more than 90% loss of activity | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 4.8 | hydrolysis of dansyl derivative of Taka-amylase A glycopeptide | Aspergillus oryzae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | in the refrigerator, stable for at least 4 months | Aspergillus oryzae |