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Literature summary for 3.2.1.96 extracted from

  • Hitomi, J.; Murakami, Y.; Saitoh, F.; Shigemitsu, N.; Yamaguchi, H.
    Purification and characterization of endo-beta-N-acetylglucosaminidase of Aspergillus oryzae (1985), J. Biochem., 98, 527-533.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 1 mM, 18% stimulation Aspergillus oryzae
L-Cys 1 mM, 18% stimulation Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0868
-
dansyl derivative of Taka-amylase A glycopeptide
-
Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
gel filtration Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-
Streptomyces griseus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Aspergillus oryzae

Storage Stability

Storage Stability Organism
stable for at least 4 months in the refrigerator at pH 4.0-6.0 Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dansyl derivative of Taka-amylase A glycopeptide + H2O
-
Aspergillus oryzae ?
-
?
additional information
-
Streptomyces griseus ?
-
?
additional information high-mannose-type glycopeptides are rapidly hydrolyzed, hybrid-type glycopeptides are good substrates, complex-type glycopeptides as well as smaller glycopeptides prepared by partial glycosidase digestion of complex-type glycopeptides are resistant to the enzyme Aspergillus oryzae ?
-
?
Taka-amylase A glycopeptide + H2O dansyl derivative of taka-amylase A glycopeptide Aspergillus oryzae additional information product contains N-acetylglucosaminitol and mannose in the molar ratio 1.0:5.2, but no N-acetylglucosamine or mannitol ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
pH 5.0, 0.1 M acetate buffer, 2 h, less than 10% loss of activity Aspergillus oryzae
60
-
10 min, more than 90% loss of activity Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.8 hydrolysis of dansyl derivative of Taka-amylase A glycopeptide Aspergillus oryzae

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6 in the refrigerator, stable for at least 4 months Aspergillus oryzae