Cloned (Comment) | Organism |
---|---|
expressed in a silkworm-baculovirus expression system | Streptomyces plicatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32900 | - |
x * 32900, calculated from amino acid sequence | Streptomyces plicatus |
33000 | - |
x * 33000, SDS-PAGE | Streptomyces plicatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces plicatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography and StrepTrap column chromatography | Streptomyces plicatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme hydrolyzes GlcNAcbeta1->4GlcNAc linkage of high mannose and hybrid type glycans and cannot cleave the linkage of complex type glycans | Streptomyces plicatus | ? | - |
? | |
N-glycosylated RNase B + H2O | the enzyme cleaves within the chitobiose core of high-mannose oligosaccharides from RNase B | Streptomyces plicatus | ? | - |
? | |
N-glycosylated silkworm hemolymph glycoproteins + H2O | - |
Streptomyces plicatus | ? | - |
? | |
N-glycosylated storage protein 2 + H2O | - |
Streptomyces plicatus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE | Streptomyces plicatus |
? | x * 32900, calculated from amino acid sequence | Streptomyces plicatus |
Synonyms | Comment | Organism |
---|---|---|
Endo H | - |
Streptomyces plicatus |
endo-beta-N-acetylglucosaminidase H | - |
Streptomyces plicatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | 47 | the enzymatic activity of the recombinant enzyme is retained at 27-47°C | Streptomyces plicatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | the enzymatic activity of the recombinant enzyme is retained at pH 4.0-7.0 | Streptomyces plicatus |