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Literature summary for 3.2.1.96 extracted from

  • Yamamoto, K.; Tanaka, T.; Fujimori, K.; Kang, C.S.; Ebihara, H.; Kanamori, J.; Kadowaki, S.; Tochikura, T.; Kumagai, H.
    Characterization of Bacillus sp. endo-beta-N-acetylglucosaminidase and its application to deglycosylation of hen ovomucoid (1998), Biotechnol. Appl. Biochem., 28, 235-242.
No PubMed abstract available
Search for more literature for 3.2.1.96

Application

Application Comment Organism
nutrition deglycosylation of hen ovomucoid, enzyme may be useful for the preparation of allergen-free egg products Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 enzyme form endo-BII, non-denaturing PAGE Bacillus sp. (in: Bacteria)
150000
-
 native PAGE, Endo-BII Bacillus sp. (in: Bacteria)
210000
-
 enzyme form endo-BI, non-denaturing PAGE Bacillus sp. (in: Bacteria)
210000
-
 native PAGE, Endo-BI Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-beta-D-hexosaminides + H2O Bacillus sp. (in: Bacteria)
-
 N-acetyl-D-hexosamines
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-

Purification (Commentary)

Purification (Comment) Organism
enzyme form endo-BI and endo-BII Bacillus sp. (in: Bacteria)
to homogeneity, Endo-BI: 18fold Bacillus sp. (in: Bacteria)

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bacillus sp. (in: Bacteria)
0.0012
-
 substrate: dansyl-ovomucoid, Endo-BI Bacillus sp. (in: Bacteria)
0.0216
-
 substrate: dansyl-ovomucoid, Endo-BI Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dansyl-ovalbumin-glycoprotein + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
dansyl-ovomucoid + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
dansyl-ovomucoid-glycoprotein + H2O
-
 Bacillus sp. (in: Bacteria) dansyl asparaginyl N-acetylglucosamine + ?
-
 ?
glycopeptide + H2O enzyme endo-BI hydrolyzes the pyridylamino-trimannosyl core and pyridylamino-agalacto biantennary complex-type oligosaccharides, enzyme form endo-BII acts specifically on the high-mannose-type oligosaccharides (Man)5-9(GlcNAc)2 present in ovomucoid in small amounts Bacillus sp. (in: Bacteria) ?
-
 ?
glycoprotein + H2O endo-BI hydrolyzes oligosaccharides of both hen ovalbumin and ovomucoid. Endo-BII can act only on oligosaccharides of hen ovalbumin and shows almost no activity towards hen ovomucoid Bacillus sp. (in: Bacteria) ?
-
 ?
N-acetyl-beta-D-hexosaminides + H2O
-
 Bacillus sp. (in: Bacteria) N-acetyl-D-hexosamines
-
 ?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Bacillus sp. (in: Bacteria) 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?

Synonyms

Synonyms Comment Organism
endo-beta-N-acetylglucosaminidase
-
-
endo-BII
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 10 min, stable up to Bacillus sp. (in: Bacteria)
40
-
 for 10 min, stable Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6 enzyme form endo-BI and endo-BII Bacillus sp. (in: Bacteria)
5.5 6 Endo-BI and -BII Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 4°C, 10 min, stable Bacillus sp. (in: Bacteria)
6 7 stable overnight when maintained at 4°C Bacillus sp. (in: Bacteria)