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Literature summary for 3.2.1.96 extracted from

  • Ito, S.; Muramatsu, T.; Kobata, A.
    endo-beta-N-Acetylglucosaminidases acting on carbohydrate moieties of glycoproteins: purification and properties of the two enzymes with different specificities from Clostridium perfringens (1975), Arch. Biochem. Biophys., 171, 78-86.
    View publication on PubMed
Search for more literature for 3.2.1.96

Inhibitors

Inhibitors Comment Organism Structure
EDTA slight inhibition Clostridium perfringens
p-chloromercuriphenyl sulfonate
-
 Clostridium perfringens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 Manalpha1->6(Manalpha1->3)Manbeta1->4GlcNAcbeta1->4(Fucalpha1->6)GlcNAc->N-acetylAsn endo-beta-N-acetylglucosaminidase CI Clostridium perfringens
0.05
-
 Manalpha1->6(Manalpha1->3)Manalpha1->6(Manalpha1->2Manalpha1->3)Manbeta1->4GlcNAcbeta1->4GlcNAcAsn endo-beta-N-acetylglucosaminidase CII Clostridium perfringens

Organism

Organism UniProt Comment Textmining
Clostridium perfringens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
endo-beta-N-acetylglucosaminidase CI and CII Clostridium perfringens

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Clostridium perfringens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.299
-
 endo-beta-N-acetylglucosaminidase CI Clostridium perfringens
0.304
-
 endo-beta-N-acetylglucosaminidase CII Clostridium perfringens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Man)6(GlcNAc)2Asn + H2O
-
 Clostridium perfringens (Man)6GlcNAc + GlcNAc-Asn
-
 ?
glycopeptide + H2O specificity Clostridium perfringens ?
-
 ?
Manalpha1->6(Manalpha1->3)Manalpha1->6(Manalpha1->2Manalpha1->3)Manbeta1->4GlcNAcbeta1->4GlcNAcAsn + H2O endo-beta-N-acetylglucosaminidase CII Clostridium perfringens Manalpha1->6(Manalpha1->3)Manalpha1->6(Manalpha1->2Manalpha1->3)Manbeta1->4GlcNAcbeta1 + GlcNAcAsn
-
 ?
Manalpha1->6(Manalpha1->3)Manbeta1->4GlcNAcbeta1->4(Fucalpha1->6)GlcNAc->N-acetylAsn + H2O endo-beta-N-acetylglucosaminidase CI Clostridium perfringens Manalpha1->6(Manalpha1->3)Manbeta1->4GlcNAc + Fucalpha1-6GlcNAc-N-acetylAsn
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 endo-beta-N-acetylglucosaminidase CI Clostridium perfringens
7
-
 endo-beta-N-acetylglucosaminidase CI Clostridium perfringens