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Literature summary for 3.2.1.96 extracted from

  • Elder, J.H.; Alexander, S.
    endo-beta-N-acetylglucosaminidase F: endoglycosidase from Flavobacterium meningosepticum that cleaves both high-mannose and complex glycoproteins (1982), Proc. Natl. Acad. Sci. USA, 79, 4540-4544.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.96

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol ovalbumin, fetuin, bromelain, ovomucoid, alpha1-acid glycoprotein, immunoglobulin G and influenza virus hemagglutinin are susceptible only after reduction and alkylation or in presence of 1% 2-mercaptoethanol Elizabethkingia meningoseptica
Nonionic detergent glycoproteins of retrovirus, lymphocytic choriomeningitis virus Elizabethkingia meningoseptica
Nonionic detergent Pichinde virus, and HLA-A and HLA-B antigens are cleaved in presence of nonionic detergent Elizabethkingia meningoseptica

Application

Application Comment Organism
analysis study of glycans and protein backbones as discrete entities and for defining the nature of the glycan-protein interface Elizabethkingia meningoseptica

Inhibitors

Inhibitors Comment Organism Structure
SDS
-
 Elizabethkingia meningoseptica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Elizabethkingia meningoseptica 5829
-

Organism

Organism UniProt Comment Textmining
Elizabethkingia meningoseptica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycoprotein + H2O high-mannose and complex glycoproteins linked through asparagine to the protein backbone, cleavage occurs via hydrolysis of the glycosidic bond of the N,N'-diacetylchiotobiose core structure adjacent to asparagine. Glycoproteins of retrovirus, lymphocytic choriomeningitis virus, Pichinde virus, and HLA-A and HLA-B antigens are cleaved in presence of nonionic detergent. Ovalbumin, fetuin, bromelain, ovomucoid, alpha1-acid glycoprotein, immunoglobulin G and influenza virus hemagglutinin are susceptible only after reduction and alkylation or in presence of 1% 2-mercaptoethanol Elizabethkingia meningoseptica ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-beta-N-acetylglucosaminidase F
-
 Elizabethkingia meningoseptica