EC Number |
General Stability |
Reference |
---|
3.2.1.96 | 20-30% loss of initial activity is lost when the protein concentration is decreased to less than 0.02-0.03 mg/ml |
171709 |
3.2.1.96 | activity decreases on repeated freezing |
171717 |
3.2.1.96 | pronase, trypsin, and chymotrypsin at 2.5 mg per ml, when incubated with 0.005-0.010 mg of enzyme per ml, has little or no effect after 5 h at 37°C. 5 mg/ml pronase inactivates slowly during a 12 h period |
171698 |
3.2.1.96 | retains nearly 30% of its activity in 1% SDS |
171686 |
3.2.1.96 | stable to freezing at -70°C |
171688, 171705 |
3.2.1.96 | stable to incubation with 250fold excess of either Pronase, chymotrypsin, or trypsin w/w. Slow inactivation by 1000fold excess of Pronase during 12 h incubation |
171686 |
3.2.1.96 | stable to lyophilization |
171678, 171709 |
3.2.1.96 | stable to repeated freezing and thawing |
171686, 171709 |
3.2.1.96 | stable to repeated freezing and thawing or lyophilization |
171698 |
3.2.1.96 | trypsin, 0.01 mg/ml, pH 7.2, 50 mM Tris/HCl, 70% inactivation after 15 min |
171691 |