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Literature summary for 3.2.1.96 extracted from

  • Takegawa, K.; Naitoh, T.; Iwahara, S.
    Isolation of two endo-beta-N-acetylglucosaminidases with different specificities from Pseudomonas sp. (1991), Biochem. Int., 24, 793-799.
    View publication on PubMed
Search for more literature for 3.2.1.96

Application

Application Comment Organism
analysis the enzyme will be an excellent tool for clarification of the structures and functions of complex oligosaccharides in glycoproteins Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
PCMB 1 mM, 84% inhibition Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
94000
-
 x * 94000, endo-beta-N-acetylglucosaminidase PI, SDS-PAGE Pseudomonas sp.
106000
-
 x * 106000, endo-beta-N-acetylglucosaminidase PI, SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
endo-beta-N-acetylglucosaminidase PI and PII Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Pseudomonas sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Man)3(GlcNAc)2-N-Asn-dansyl + H2O endo-beta-N-acetylglucosaminidase PII, no activity with endo-beta-N-acetylglucosaminidase PII Pseudomonas sp. ?
-
 ?
additional information endo-beta-N-acetylglucosaminidase PI hydrolyzes high-mannose type oligosaccharides GP-IV and GP-V faster than the hybrid type FP-I, GP-II, and GP-III. Endo-beta-N-acetylglucosaminidase PII hydrolyzes high mannose and hybrid type oligosaccharides Pseudomonas sp. ?
-
 ?

Subunits

Subunits Comment Organism
? x * 106000, endo-beta-N-acetylglucosaminidase PI, SDS-PAGE Pseudomonas sp.
? x * 94000, endo-beta-N-acetylglucosaminidase PI, SDS-PAGE Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 10 min, 5% loss of activity, endo-beta-N-acetylglucosaminidase PII Pseudomonas sp.
50
-
 10 min, 60% loss of activity, endo-beta-N-acetylglucosaminidase PII Pseudomonas sp.
55
-
 10 min, stable, endo-beta-N-acetylglucosaminidase I Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6 ENGase PI Pseudomonas sp.
6 8 ENGase PII Pseudomonas sp.