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Literature summary for 3.2.1.96 extracted from

  • Fischl, R.M.; Stadlmann, J.; Grass, J.; Altmann, F.; Leonard, R.
    The two endo-beta-N-acetylglucosaminidase genes from Arabidopsis thaliana encode cytoplasmic enzymes controlling free N-glycan levels (2011), Plant Mol. Biol., 77, 275-284.
    View publication on PubMed
Search for more literature for 3.2.1.96

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Arabidopsis thaliana 5737
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 ecotype Col-0
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Arabidopsis thaliana
-
leaf in adult leaves the predominance of isoform ENGase85A accounts for 80% of the total activity Arabidopsis thaliana
-
leaflet
-
 Arabidopsis thaliana
-
additional information no enzyme activity is detected in siliques Arabidopsis thaliana
-
root
-
 Arabidopsis thaliana
-
rootlet highest activity in rootlets Arabidopsis thaliana
-
stem isoform ENGase85A only Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycosylated ascorbate oxidase + H2O
-
 Arabidopsis thaliana ?
-
 ?
glycosylated human apo-transferrin + H2O
-
 Arabidopsis thaliana ?
-
 ?
glycosylated mAb 2G12 + H2O
-
 Arabidopsis thaliana ?
-
 ?
glycosylated type VI-A horseraddish peroxidase + H2O
-
 Arabidopsis thaliana ?
-
 ?
lectin + H2O
-
 Arabidopsis thaliana ?
-
 ?
ribonuclease B + H2O
-
 Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-beta-N-acetylglucosaminidase
-
 Arabidopsis thaliana
ENGase85A isoform Arabidopsis thaliana
ENGase85B isoform Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 isoform ENGase85A Arabidopsis thaliana
7
-
 isoform ENGase85B Arabidopsis thaliana