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Literature summary for 3.2.1.96 extracted from

  • Murakami, S.; Takaoka, Y.; Ashida, H.; Yamamoto, K.; Narimatsu, H.; Chiba, Y.
    Identification and characterization of endo-beta-N-acetylglucosaminidase from methylotrophic yeast Ogataea minuta (2013), Glycobiology, 23, 736-744.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Ogataea minuta Ogataea minuta

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Ogataea minuta
Fe2+
-
Ogataea minuta
additional information not inhibited by EDTA or EGTA Ogataea minuta
Zn2+
-
Ogataea minuta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.54
-
9-fluorenylmethyloxycarbonyl-labeled asparagine-linked agalactobiantennary oligosaccharide at pH 5.5 and 50°C Ogataea minuta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
87398
-
x * 87398, calculated from amino acid sequence Ogataea minuta
91000
-
x * 91000, SDS-PAGE Ogataea minuta

Organism

Organism UniProt Comment Textmining
Ogataea minuta R4WHQ8
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap HP affinity column chromatography Ogataea minuta

Storage Stability

Storage Stability Organism
-20°C, 10% (v/v) glycerol solution at pH 7.4, 5 months, almost 100% of the activity is retained Ogataea minuta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
9-fluorenylmethyloxycarbonyl-labeled asparagine-linked agalactobiantennary oligosaccharide + 4-nitrophenyl-beta-D-glucopyranoside transglycosylation activity Ogataea minuta ?
-
?
9-fluorenylmethyloxycarbonyl-labeled asparagine-linked agalactobiantennary oligosaccharide + H2O
-
Ogataea minuta 9-fluorenylmethyloxycarbonyl-labeled asparagine-linked N-acetylglucosamine (GlcNAc) + ?
-
?
additional information the enzyme exhibits hydrolytic activity for high-mannose, hybrid, biantennary and (2,6)-branched triantennary N-linked oligosaccharides, but not for tetraantennary, (2,4)-branched triantennary, bisecting N-acetylglucosamine structure and core-fucosylated biantennary N-linked oligosaccharides. The enzyme shows almost the same relative activity for both biantennary (37%) and (2,6)-triantennary oligosaccharides (35%), and also for both hybrid (10.6%) and asialo biantennary (8.3%) oligosaccharides Ogataea minuta ?
-
?
N-glycosylated RNase B + H2O
-
Ogataea minuta ?
-
?
N-glycosylated transferrin + H2O
-
Ogataea minuta ?
-
?

Subunits

Subunits Comment Organism
? x * 91000, SDS-PAGE Ogataea minuta
? x * 87398, calculated from amino acid sequence Ogataea minuta

Synonyms

Synonyms Comment Organism
endo-beta-N-acetylglucosaminidase
-
Ogataea minuta
ENGase
-
Ogataea minuta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Ogataea minuta

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.91
-
9-fluorenylmethyloxycarbonyl-labeled asparagine-linked agalactobiantennary oligosaccharide at pH 5.5 and 50°C Ogataea minuta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Ogataea minuta

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8 the activity is decreased less than 20% at pH 4.5 or 8.0 Ogataea minuta

pI Value

Organism Comment pI Value Maximum pI Value
Ogataea minuta calculated from amino acid sequence
-
5.6