Cloned (Comment) | Organism |
---|---|
expressed in Ogataea minuta | Ogataea minuta |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Ogataea minuta | |
Fe2+ | - |
Ogataea minuta | |
additional information | not inhibited by EDTA or EGTA | Ogataea minuta | |
Zn2+ | - |
Ogataea minuta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.54 | - |
9-fluorenylmethyloxycarbonyl-labeled asparagine-linked agalactobiantennary oligosaccharide | at pH 5.5 and 50°C | Ogataea minuta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87398 | - |
x * 87398, calculated from amino acid sequence | Ogataea minuta |
91000 | - |
x * 91000, SDS-PAGE | Ogataea minuta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea minuta | R4WHQ8 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap HP affinity column chromatography | Ogataea minuta |
Storage Stability | Organism |
---|---|
-20°C, 10% (v/v) glycerol solution at pH 7.4, 5 months, almost 100% of the activity is retained | Ogataea minuta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
9-fluorenylmethyloxycarbonyl-labeled asparagine-linked agalactobiantennary oligosaccharide + 4-nitrophenyl-beta-D-glucopyranoside | transglycosylation activity | Ogataea minuta | ? | - |
? | |
9-fluorenylmethyloxycarbonyl-labeled asparagine-linked agalactobiantennary oligosaccharide + H2O | - |
Ogataea minuta | 9-fluorenylmethyloxycarbonyl-labeled asparagine-linked N-acetylglucosamine (GlcNAc) + ? | - |
? | |
additional information | the enzyme exhibits hydrolytic activity for high-mannose, hybrid, biantennary and (2,6)-branched triantennary N-linked oligosaccharides, but not for tetraantennary, (2,4)-branched triantennary, bisecting N-acetylglucosamine structure and core-fucosylated biantennary N-linked oligosaccharides. The enzyme shows almost the same relative activity for both biantennary (37%) and (2,6)-triantennary oligosaccharides (35%), and also for both hybrid (10.6%) and asialo biantennary (8.3%) oligosaccharides | Ogataea minuta | ? | - |
? | |
N-glycosylated RNase B + H2O | - |
Ogataea minuta | ? | - |
? | |
N-glycosylated transferrin + H2O | - |
Ogataea minuta | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 91000, SDS-PAGE | Ogataea minuta |
? | x * 87398, calculated from amino acid sequence | Ogataea minuta |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-N-acetylglucosaminidase | - |
Ogataea minuta |
ENGase | - |
Ogataea minuta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Ogataea minuta |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.91 | - |
9-fluorenylmethyloxycarbonyl-labeled asparagine-linked agalactobiantennary oligosaccharide | at pH 5.5 and 50°C | Ogataea minuta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Ogataea minuta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | the activity is decreased less than 20% at pH 4.5 or 8.0 | Ogataea minuta |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ogataea minuta | calculated from amino acid sequence | - |
5.6 |