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Literature summary for 3.2.1.96 extracted from

  • Fujita, K.; Nakatake, R.; Yamabe, K.; Watanabe, A.; Asada, Y.; Takegawa, K.
    Identification of amino acid residues essential for the substrate specificity of Flavobacterium sp. endo-beta-N-acetylglucosaminidase (2001), Biosci. Biotechnol. Biochem., 65, 1542-1548.
    View publication on PubMed
Search for more literature for 3.2.1.96

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Flavobacterium sp.

Protein Variants

Protein Variants Comment Organism
D121N mutant has no enzymatic activity Flavobacterium sp.
D124N mutant has 35% of the wild-type activity Flavobacterium sp.
D126E mutant has 0.1% of the wild-type activity Flavobacterium sp.
D126N mutant has 2% of the wild-type activity Flavobacterium sp.
D127A mutant has 1.8% of the wild-type activity Flavobacterium sp.
D127E mutant has 7.2% of the wild-type activity Flavobacterium sp.
D127N mutant has 6% of the wild-type activity Flavobacterium sp.
E128A mutant has no enzymatic activity Flavobacterium sp.
F42G mutant has 30% of the wild-type activity Flavobacterium sp.
G122L mutant has 4.4% of the wild-type activity Flavobacterium sp.
N17A mutant has 80% of the wild-type activity Flavobacterium sp.
N45A mutant has 70% of the wild-type activity Flavobacterium sp.
N58A mutant has 100% of the wild-type activity Flavobacterium sp.
N60A mutant has 100% of the wild-type activity Flavobacterium sp.
N89S mutant has 100% of the wild-type activity Flavobacterium sp.
Q91A mutant has 100% of the wild-type activity Flavobacterium sp.
V16A mutant has 70% of the wild-type activity Flavobacterium sp.
Y129A mutant has 70% of the wild-type activity Flavobacterium sp.
Y13A mutant has 0.1% of the wild-type activity Flavobacterium sp.
Y164A mutant has 70% of the wild-type activity Flavobacterium sp.
Y191A mutant has 0.2% of the wild-type activity Flavobacterium sp.
Y241A mutant has 20% of the wild-type activity Flavobacterium sp.
Y56A mutant has 100% of the wild-type activity Flavobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 SDS-PAGE Flavobacterium sp.

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Flavobacterium sp.

Renatured (Commentary)

Renatured (Comment) Organism
after denaturation with 8 M urea Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dansyl-Asn-(GlcNAc)2(Man)6 + H2O
-
 Flavobacterium sp. dansyl-Asn-GlcNAc + (Man)6GlcNAc
-
 ?

Synonyms

Synonyms Comment Organism
Endo-Fsp
-
 Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 9 enzyme is most active within this range Flavobacterium sp.