Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.96 extracted from

  • Koide, N.; Muramatsu, T.
    endo-beta-N-Acetylglucosaminidase acting on carbohydrate moieties of glycoproteins. Purification and properties of the enzyme from Diplococcus pneumoniae (1974), J. Biol. Chem., 249, 4897-4904.
    View publication on PubMed
Search for more literature for 3.2.1.96

Inhibitors

Inhibitors Comment Organism Structure
N-acetylglucosamino(1->5)lactone
-
 Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 Asn(GlcNAc)2-(Man)5
-
 Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus pneumoniae

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Streptococcus pneumoniae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.67
-
-
 Streptococcus pneumoniae

Storage Stability

Storage Stability Organism
-20°C, protein concentration more than 0.5 mg/ml, less than 30% loss of activity after 2 months Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Asn(GlcNAc)2-(Man)5 + H2O
-
 Streptococcus pneumoniae AsnGlcNAc + Man5(GlcNAc)
-
 ?
glycopeptide + H2O specificity Streptococcus pneumoniae ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 hydrolysis of Asn(GlcNAc)2-(Man)5 Streptococcus pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
5.3 8.2 about 50% of maximal activity at pH 5.3 and at pH 8.2 Streptococcus pneumoniae