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Literature summary for 3.2.1.96 extracted from
- Purification and properties of an endo-beta-N-acetylglucosaminidase from hen oviduct (1976), J. Biol. Chem., 251, 6537-6543.
General Stability
| General Stability | Organism |
|---|---|
| stable to freezing at -70°C | Gallus gallus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
gel filtration | Gallus gallus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Gallus gallus | probably responsible for hydrolysis of both fucose-containing and fucose-depleted substrates | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
hen | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| oviduct | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Gallus gallus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 6 months, 10-15% loss of activity | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Asn(GlcNAc)2(Man)3 + H2O | - |
Gallus gallus | ? | - |
? | |
| Asn(GlcNAc)2-(Man)5 + H2O | - |
Gallus gallus | AsnGlcNAc + Man5(GlcNAc) | - |
? | |
| Asn(GlcNAc)2-(Man)6 + H2O | - |
Gallus gallus | AsnGlcNAc + (GlcNAc)1-(Man)6 | - |
? | |
| dansyl-Asn-(GlcNAc)2(Man)6 + H2O | - |
Gallus gallus | dansyl-Asn-GlcNAc + (Man)6GlcNAc | - |
? | |
| glycopeptide + H2O | glycopeptide from immunoglobulin M, (aa)x-Asn(GlcNAC)2(Man)3(Fuc)1 | Gallus gallus | ? | - |
? | |
| additional information | probably responsible for hydrolysis of both fucose-containing and fucose-depleted substrates | Gallus gallus | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
24 h, 30% loss of activity | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
hydrolysis of dansyl-Asn(GlcNAc)2-(Man)6 | Gallus gallus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | pH 5.5: maximal activity, pH 9.5: about 40% of maximal activity, hydrolysis of dansyl-Asn(GlcNAc)2(Man)6 | Gallus gallus |
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