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Literature summary for 3.2.1.96 extracted from
- Purification and substrate specificity of an endo-beta-N-acetylglucosaminidase from pea (Pisum sativum) seeds (1996), Biosci. Biotechnol. Biochem., 60, 228-232.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 10 mM, 75% inhibition | Pisum sativum |  |
| Cu2+ | 10 mM, 75% inhibition | Pisum sativum | |
| Fe3+ | 10 mM, 75% inhibition | Pisum sativum | |
| Urea | 50% inhibition by 2 M, 65% inhibition at 4 M | Pisum sativum | |
| Zn2+ | 10 mM, 50% inhibition | Pisum sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
(Man)6(GlcNAc)2-pyridylamino | - |
Pisum sativum | |
| 0.32 | - |
(Man)9(GlcNAc)2-pyridylamino | - |
Pisum sativum | |
| 0.4 | - |
(Man)5(GlcNAc)2-pyridylamino | - |
Pisum sativum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41670 | - |
MALDI-TOF mass spectrometry | Pisum sativum |
| 42000 | - |
1 * 42000, SDS-PAGE | Pisum sativum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 400 | - |
- |
Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (Man)5GlcNAc2-pyridylamino + H2O | - |
Pisum sativum | ? | - |
? | |
| (Man)6GlcNAc2-pyridylamino + H2O | - |
Pisum sativum | ? | - |
? | |
| (Man)9GlcNAc2-pyridylamino + H2O | - |
Pisum sativum | ? | - |
? | |
| glycoprotein + H2O | - |
Pisum sativum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, SDS-PAGE | Pisum sativum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pisum sativum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 60 | 25°C: about 80% of maximal activity, 60°C: about 45% of maximal activity | Pisum sativum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | stable | Pisum sativum |
| 50 | - |
unstable | Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
hydrolysis of (Man)6(GlcNAc)2-pyridylamino | Pisum sativum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | about 55% of maximal activity at pH 5.0 and at pH 8.5 | Pisum sativum |
| 6 | 7.5 | 80% of maximal activity at pH 6.0 and at pH 7.5 | Pisum sativum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | stable | Pisum sativum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
Zn2+ | - |
Pisum sativum | |
| 2000 | - |
Urea | - |
Pisum sativum | |
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