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4.2.2.2: pectate lyase

This is an abbreviated version!
For detailed information about pectate lyase, go to the full flat file.

Word Map on EC 4.2.2.2

Reaction

[pectate]n
=
4-deoxy-alpha-D-gluc-4-enuronosyl-1,4-[pectate]n-1
 +
Alpha-D-glucuronic acid

Synonyms

alkaline pectate lyase, alkaline polygalacturonate lyase, alpha-1,4-D-endopolygalacturonic acid lyase, Apel, Athe_1854, AtPLL, Bsp165PelA, BxPEL1, BxPEL2, C0W65_13990, Calkro_0863, Cthe_2179, DKS1 pectate lyase, EC 4.2.99.3, endo-alpha-1,4-polygalacturonic acid lyase, endo-polygalacturonate trans-eliminase, endogalacturonate transeliminase, endopectin methyltranseliminase, FcPL1, Gr-PEL-2, Gr-PEL2, Hb-PEL-1, Hspel1, Hspel2, lyase, pectate, MdPL1, More, neutral-alkaline pectate lyase, NPLase, PATE, PCPEL2, pectate lyase, pectate lyase 10A, pectate lyase 2, pectate lyase 9A, pectate lyase A, pectate lyase B, pectate lyase C, pectate lyase E, pectate lyase I, pectate lyase L, pectate lyase Pel10A, pectate lyase Pel9A, pectate lyase PL 47, pectate transeliminase, pectic acid lyase, pectic acid transeliminase, pectic lyase, Pel, Pel I, Pel II, Pel III, Pel SWU, Pel-15, Pel-15E, Pel-15H, pel-2, Pel-20, Pel-22, Pel-66, Pel-90, Pel-BL11, Pel1, Pel10A, PEL168, Pel9A, PelA, PelB, pelC, PelD, PelE, PelI, PelN, PGA lyase, PGL, PL, PL D, PL I, PL-3, PL-STR, PL1B, Pla, PLB, PLC, PLE, poly(1,4-alpha-D-galacturonide) lyase, poly-galacturonic acid trans-eliminase, polygalacturonate lyase, polygalacturonic acid lyase, polygalacturonic transeliminase, PPase-N, ScPL NP_626147, ScPL NP_627050, XcPL NP_636037, XcPL NP_638163

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.2 pectate lyase

Specific Activity

Specific Activity on EC 4.2.2.2 - pectate lyase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.065
culture filtrate, in 20 mM Tris-HCl pH 8.0, at 60°C
1000
after 25.3fold purification
12
after 185fold purification, in 20 mM Tris-HCl pH 8.0, at 60°C
124
-
pH 10, 50°C
1252
pH 9.4, 50°C
130.9
EDW21517
wild-type, pH 10, 60°C
1320
purified recombinant enzyme from Pichia pastoris, pH 9.5, 50°C
144.4
EDW21517
mutant R227W, pH 10, 60°C
1493
recombinant protein purified from inclusion bodies, pH 9.0, 50°C
153
substrate pectin, 55-70% methylated, pH 10.5, 70°C
157
substrate pectin, 20-34% methylated, pH 10.5, 70°C
1602
wild-type, pH 9.8, 50°C
1623
purified recombinant His-tagged Pel-BL11, pH 10.0, 50°C
177
purified native enzyme
185.9
EDW21517
mutant R227W/S145C/T50T, pH 10, 60°C
1870
wild-type, pH 10.5, 50°C
194.3
-
purified enzyme, pH 9.0, 60°C
1984
purified recombinant His-tagged enzyme, unmethylated polygalacturonic acid, pH 9.8, 45°C
2060
purified recombinant enzyme, pH 9.8, 65°C
2096
purified recombinant His-tagged enzyme, 20-34% methylated polygalacturonic acid, pH 9.8, 45°C
211
substrate citrus pectin, pH 10.5, 70°C
21889
-
pH 9.0, 55°C
2305
mutant N186D/I211V/A254T/S271G/N289H, pH 10.5, 50°C
232
substrate apple pectin, pH 10.5, 70°C
2797
mutant K93I, pH 9.8, 50°C
2893
purified recombinant wild-type enzyme, pH 8.5, 60°C
293
mutant 124I/T178A/N186D/I211V/A254T/S271G/N289H, pH 10.5, 50°C
3.03
purified recombinant enzyme
3194
recombinant protein purified from soluble fraction, pH 9.0, 50°C
320
substrate polygalacturonate, pH 10.5, 70°C
326.8
EDW21517
mutant R227W/T206A/T222T, pH 10, 60°C
353.6
wild-type, pH 9.5, 55°C
438.7
EDW21517
mutant R227W/S145C/T206A, pH 10, 60°C
444.6
-
purified recombinant enzyme, pH 9.0, 50°C
614.7
purified recombinant His-tagged enzyme, 50-70% methylated pectin, pH 9.8, 45°C
668.2
purified recombinant His-tagged enzyme, over 80% methylated pectin, pH 9.8, 45°C
750
purified recombinant PCPEL2 expressed from pLysS, pH not specified in the publication, temperature not specified in the publication
852
-
purified native enzyme, pH 8.5, 55°C
860
purified recombinant PCPEL2 expressed from pMAL, pH not specified in the publication, temperature not specified in the publication
additional information