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Literature summary for 4.2.2.2 extracted from
- Another pectate lyase produced by Streptomyces nitrosporeus (1980), Agric. Biol. Chem., 44, 1345-1349.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Streptomyces nitrosporeus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Streptomyces nitrosporeus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
x * 41000, SDS-PAGE | Streptomyces nitrosporeus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces nitrosporeus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces nitrosporeus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 319 | - |
- |
Streptomyces nitrosporeus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hexagalacturonic acid | 140% of the activity with low molecular weight polygalacturonic acid | Streptomyces nitrosporeus | ? | - |
? | |
| additional information | macerating activity on potato tissue and Ganpi bark | Streptomyces nitrosporeus | ? | - |
? | |
| pentagalacturonic acid | 215% of the activity with low molecular weight polygalacturonic acid | Streptomyces nitrosporeus | saturated digalacturonic acid + unsaturated trigalacturonic acid + saturated trigalacturonic acid + unsaturated digalacturonic acid | - |
? | |
| polygalacturonic acid | random attack | Streptomyces nitrosporeus | unsaturated oligogalacturonate | higher oligomers are detected in the early stage of degradation, unsaturated monogalacturonic acids and digalacturonic acids are also detected after prolonged degradation | ? | |
| polygalacturonic acid | low molecular weight. High molecular weight polygalacturonic acid is attacked at 46% of the activity with low molecular weight polygalacturonic acid | Streptomyces nitrosporeus | unsaturated oligogalacturonate | higher oligomers are detected in the early stage of degradation, unsaturated monogalacturonic acids and digalacturonic acids are also detected after prolonged degradation | ? | |
| tetragalacturonic acid | 141% of the activity with low molecular weight polygalacturonic acid | Streptomyces nitrosporeus | altered trigalacturonic acid + digalacturonic acid + altered digalacturionic acid + D-galacturonic acid | - |
? | |
| trigalacturonic acid | 3% of the activity with low molecular weight polygalacturonic acid | Streptomyces nitrosporeus | altered digalacturonic acid + galacturonic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41000, SDS-PAGE | Streptomyces nitrosporeus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
in a 2 min reaction at pH 9.5 | Streptomyces nitrosporeus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
pH 7.0, 10 min, stable below | Streptomyces nitrosporeus |
| 50 | - |
10 min, pH 7.0, 35% loss of activity | Streptomyces nitrosporeus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Streptomyces nitrosporeus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | 2°C, 48 h, stable | Streptomyces nitrosporeus |
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Release 2023.1 (January 2023)
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