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Literature summary for 4.2.2.2 extracted from
- Purification and properties of pectate lyase produced by Streptomyces nitrosporeus (1977), Agric. Biol. Chem., 41, 2193-2197.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km for acid soluble polygalacturonic acid: 0.051% | Streptomyces nitrosporeus | |
| 0.19 | - |
tetragalacturonic acid | - |
Streptomyces nitrosporeus |  |
| 0.23 | - |
pentagalacturonic acid | - |
Streptomyces nitrosporeus | |
| 0.38 | - |
hexagalacturonic acid | - |
Streptomyces nitrosporeus | |
| 1.5 | - |
trigalacturonic acid | - |
Streptomyces nitrosporeus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | cation required, Ca2+ is most effective | Streptomyces nitrosporeus | |
| Mg2+ | 26% of the activation with Ca2+ | Streptomyces nitrosporeus | |
| Mn2+ | 27% of the activity with Ca2+ | Streptomyces nitrosporeus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
gel filtration | Streptomyces nitrosporeus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces nitrosporeus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces nitrosporeus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Streptomyces nitrosporeus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hexagalacturonic acid | - |
Streptomyces nitrosporeus | ? | - |
? | |
| additional information | digalacturonic acid and trigalacturonic acid are not good substrates | Streptomyces nitrosporeus | ? | - |
? | |
| additional information | no macerating activity on Gampi bark, carrot, potato, cabbage and radish | Streptomyces nitrosporeus | ? | - |
? | |
| pentagalacturonic acid | - |
Streptomyces nitrosporeus | saturated digalacturonic acid + unsaturated trigalacturonic acid + saturated trigalacturonic acid + unsaturated digalacturonic acid | - |
? | |
| polygalacturonic acid | - |
Streptomyces nitrosporeus | unsaturated oligogalacturonate | - |
? | |
| tetragalacturonic acid | - |
Streptomyces nitrosporeus | altered trigalacturonic acid + digalacturonic acid + altered digalacturionic acid + D-galacturonic acid | - |
? | |
| trigalacturonic acid | - |
Streptomyces nitrosporeus | altered digalacturonic acid + galacturonic acid | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.3 | 9.5 | - |
Streptomyces nitrosporeus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | 2°C, 72 h, stable | Streptomyces nitrosporeus |
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Release 2023.1 (January 2023)
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