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Literature summary for 4.2.2.2 extracted from
- Inducible thermoalkalophilic polygalacturonate lyase from Thermomonospora fusca (1987), J. Bacteriol., 169, 2774-2780.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 0.0022 mM, 50% inhibition | Thermobifida fusca |  |
| Cd2+ | - |
Thermobifida fusca | |
| dithiothreitol | 0.0104 mM, 50% inhibition | Thermobifida fusca | |
| Hg2+ | - |
Thermobifida fusca | |
| Pb2+ | - |
Thermobifida fusca | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the Km-value for pectin increases 30000fold as the percent esterification of the substrate increases from 0 to 60% | Thermobifida fusca |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Thermobifida fusca | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Thermobifida fusca | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, SDS-PAGE | Thermobifida fusca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermobifida fusca | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| bubbling O2 through the enzyme preparation increases activity 63% | Thermobifida fusca |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | contains 0.195 mg of glucose equivalents per mg of protein | Thermobifida fusca |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermobifida fusca |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Thermobifida fusca | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Thermobifida fusca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin | - |
Thermobifida fusca | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE | Thermobifida fusca |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Thermobifida fusca |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Ca2+ slightly enhances thermal stability | Thermobifida fusca |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
- |
Thermobifida fusca |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0022 | - |
2-mercaptoethanol | - |
Thermobifida fusca | |
| 0.0104 | - |
dithiothreitol | - |
Thermobifida fusca | |
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Release 2023.1 (January 2023)
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