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4.2.2.2: pectate lyase

This is an abbreviated version!
For detailed information about pectate lyase, go to the full flat file.

Word Map on EC 4.2.2.2

Reaction

[pectate]n
=
4-deoxy-alpha-D-gluc-4-enuronosyl-1,4-[pectate]n-1
 +
Alpha-D-glucuronic acid

Synonyms

alkaline pectate lyase, alkaline polygalacturonate lyase, alpha-1,4-D-endopolygalacturonic acid lyase, Apel, Athe_1854, AtPLL, Bsp165PelA, BxPEL1, BxPEL2, C0W65_13990, Calkro_0863, Cthe_2179, DKS1 pectate lyase, EC 4.2.99.3, endo-alpha-1,4-polygalacturonic acid lyase, endo-polygalacturonate trans-eliminase, endogalacturonate transeliminase, endopectin methyltranseliminase, FcPL1, Gr-PEL-2, Gr-PEL2, Hb-PEL-1, Hspel1, Hspel2, lyase, pectate, MdPL1, More, neutral-alkaline pectate lyase, NPLase, PATE, PCPEL2, pectate lyase, pectate lyase 10A, pectate lyase 2, pectate lyase 9A, pectate lyase A, pectate lyase B, pectate lyase C, pectate lyase E, pectate lyase I, pectate lyase L, pectate lyase Pel10A, pectate lyase Pel9A, pectate lyase PL 47, pectate transeliminase, pectic acid lyase, pectic acid transeliminase, pectic lyase, Pel, Pel I, Pel II, Pel III, Pel SWU, Pel-15, Pel-15E, Pel-15H, pel-2, Pel-20, Pel-22, Pel-66, Pel-90, Pel-BL11, Pel1, Pel10A, PEL168, Pel9A, PelA, PelB, pelC, PelD, PelE, PelI, PelN, PGA lyase, PGL, PL, PL D, PL I, PL-3, PL-STR, PL1B, Pla, PLB, PLC, PLE, poly(1,4-alpha-D-galacturonide) lyase, poly-galacturonic acid trans-eliminase, polygalacturonate lyase, polygalacturonic acid lyase, polygalacturonic transeliminase, PPase-N, ScPL NP_626147, ScPL NP_627050, XcPL NP_636037, XcPL NP_638163

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.2 pectate lyase

Cloned

Cloned on EC 4.2.2.2 - pectate lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, expression of His-tagged Pel-BL11 in Escherichia coli pET25b
DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109
-
DNA and amino acid sequence determnination and analysis
enzyme from strain ATCC49397 overproduced in Escherichia coli
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) and DH5alpha cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli JM109 cells
-
expressed in Escherichia coli Origami cells
expressed in the transgenic strawberry lines Pel 1 and Pel 3
-
expression in Bacillus licheniformis
expression in Escherichia coli
expression in Pichia pastoris
full-length enzyme and catalytic modules CM9-1 and CM9-2 are separately expressed in Escherichia coli
gene Bsu11286Pel, DNA and amino acid sequence determination and analysis, recombinant expression in Bacillus subtilis strain WB600
-
gene encoding pectate lyase is amplified by PCR, fused with a periplasmic secretion signal peptide sequence, pelB, from pET22b(+), cloned and expressed in Escherichia coli cells using a temperature control vector, pHsh
-
gene Pcpel2, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli BL21(DE3) using the pMAL or pLysS system, expression of maltose binding fusion protein, MBP-PCPEL2
gene pecCl1, DNA amino acid sequence determination and analysis, phylogenetic analysis, possible cis-regulatory elements and transcription factor binding sites that may be involved in the regulation of genetic expression were detected in the promoter region of the gene
gene pel-2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
gene pel168, enzyme expression in Pichia pastoris strain and in Escherichia coli, the latter shows less glycosylation and thermostability
gene pelA from isolate US-41, DNA and amino acid sequence determinationand analysis, expression and promoter analysis, overexpression of CcpelA in Si-60 under the regulation of the promoter Pgpd. The CcpelA promoter of US-41, but not Si-60, contains a putative AreA-binding motif, GATA box. Point mutation in the Si-60 GATA box might prevent positive regulation of CcpelA by the AreA transcription factor, leading to slower gene expression and delayed CcPelA secretion
-
gene pelB, DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) plysS
gene pelB, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain DH5alpha, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene pelN, DNA and amino acid sequence determination and analysis, recombinant enzyme expression in recombinant Escherichia coli strain BL21(DE3)
gene pelN, overexpression in Escherichia coli strain BL21(DE3) in the periplasmic space from pNA13
-
gene pl-str, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3), subcloning in Escherichia coli strain JM109
-
Hspel1 full-length cDNA sequence
Hspel2 full-length cDNA sequence
none of the 10 Pseudomonas syringae pv. glycinea strains examined exhibits pectolytic activity. The pectate lyase gene is detected in four out of four strains examined. The pel gene contains a single-base insertion, a double-base insertion, and an 18-bp deletion, which can lead to the synthesis of an inactive pectate lyase protein. The function of the protein can be restored by removing the unwanted base insertion and by filling in the 18-bp deletions by site-directed mutagenesis and expression in Escherichia coli
-
overexpressed using a promoter fusion with the Aspergillus niger pyruvate kinase promoter
overproduced in Escherichia coli
-
pectate lyase A, pectate lyase B, pectate lyase C and pectate lyase E, expression in Escherichia coli
-
pectate lyase B
-
pectate lyase B, expression in Pichia pastoris
-
pectate lyase C, expression in Pichia pastoris
-
pectate lyase D, expression in Pichia pastoris
-
pectate lyase gene fused to the pelB gene encoding the periplasmic secretion signal and the fusion gene is expressed under the control of the T7 promoter in a culture of Escherichia coli BL21DE3
-
recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
recombinantly expressed in Pichia pastoris
recombinantly expressed in Pichia pastoris. The ratio of methanol to cell concentration has a significant influence on PGL production. An advanced glycerol exponential feeding strategy is developed for biomass accumulation in cell growth phase, by which cell concentration reaches 140 g/L after 19 h glycerol feeding. In subsequent production phase, a methanol feeding profile is proposed according to the optimal ratio of methanol to cell concentration at a range of 0.163-0.171 g/g, and PGL activity and productivity reaches 430 U/ml and 4.34 U/ml/h, respectively
-
transient expression of Gr-Pel2 in leaves of Nicotiana benthamiana results in severe malformations of the infiltrated tissues, not relating to maceration and soft rot symptom
-