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Literature summary for 4.2.2.2 extracted from
- Cloning of the gene encoding an endo-acting pectate lyase from Streptomyces thermocarboxydus (2010), Biosci. Biotechnol. Biochem., 74, 433-436.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Origami cells | Streptomyces thermocarboxydus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | dependent on | Streptomyces thermocarboxydus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23900 | - |
mature enzyme, electrospray ionization mass spectrometry | Streptomyces thermocarboxydus |
| 23960 | - |
mature enzyme, calculated from amino acid sequence | Streptomyces thermocarboxydus |
| 28000 | - |
recombinant His-tagged enzyme, SDS-PAGE | Streptomyces thermocarboxydus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces thermocarboxydus | C6L867 | - |
- |
| Streptomyces thermocarboxydus B1 | C6L867 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography, gel filtration | Streptomyces thermocarboxydus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.03 | - |
purified recombinant enzyme | Streptomyces thermocarboxydus |
| 177 | - |
purified native enzyme | Streptomyces thermocarboxydus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonate | - |
Streptomyces thermocarboxydus | unsaturated 4,5-digalacturonate + unsaturated 4,5-trigalacturonate | - |
? | |
| polygalacturonate | - |
Streptomyces thermocarboxydus B1 | unsaturated 4,5-digalacturonate + unsaturated 4,5-trigalacturonate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PelA | - |
Streptomyces thermocarboxydus |
| PL I | an endo-acting family 3 pectate lyase | Streptomyces thermocarboxydus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 40 | inactivation of recombinant PL I occurs above 30°C whereas the activity of native PL I is stable up to 40°C | Streptomyces thermocarboxydus |
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