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4.2.2.2: pectate lyase

This is an abbreviated version!
For detailed information about pectate lyase, go to the full flat file.

Word Map on EC 4.2.2.2

Reaction

[pectate]n
=
4-deoxy-alpha-D-gluc-4-enuronosyl-1,4-[pectate]n-1
+
Alpha-D-glucuronic acid

Synonyms

alkaline pectate lyase, alkaline polygalacturonate lyase, alpha-1,4-D-endopolygalacturonic acid lyase, Apel, Athe_1854, AtPLL, Bsp165PelA, BxPEL1, BxPEL2, C0W65_13990, Calkro_0863, Cthe_2179, DKS1 pectate lyase, EC 4.2.99.3, endo-alpha-1,4-polygalacturonic acid lyase, endo-polygalacturonate trans-eliminase, endogalacturonate transeliminase, endopectin methyltranseliminase, FcPL1, Gr-PEL-2, Gr-PEL2, Hb-PEL-1, Hspel1, Hspel2, lyase, pectate, MdPL1, More, neutral-alkaline pectate lyase, NPLase, PATE, PCPEL2, pectate lyase, pectate lyase 10A, pectate lyase 2, pectate lyase 9A, pectate lyase A, pectate lyase B, pectate lyase C, pectate lyase E, pectate lyase I, pectate lyase L, pectate lyase Pel10A, pectate lyase Pel9A, pectate lyase PL 47, pectate transeliminase, pectic acid lyase, pectic acid transeliminase, pectic lyase, Pel, Pel I, Pel II, Pel III, Pel SWU, Pel-15, Pel-15E, Pel-15H, pel-2, Pel-20, Pel-22, Pel-66, Pel-90, Pel-BL11, Pel1, Pel10A, PEL168, Pel9A, PelA, PelB, pelC, PelD, PelE, PelI, PelN, PGA lyase, PGL, PL, PL D, PL I, PL-3, PL-STR, PL1B, Pla, PLB, PLC, PLE, poly(1,4-alpha-D-galacturonide) lyase, poly-galacturonic acid trans-eliminase, polygalacturonate lyase, polygalacturonic acid lyase, polygalacturonic transeliminase, PPase-N, ScPL NP_626147, ScPL NP_627050, XcPL NP_636037, XcPL NP_638163

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.2 pectate lyase

pH Stability

pH Stability on EC 4.2.2.2 - pectate lyase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
-
37°C, 1 h, 6% loss of activity
37372
10 - 10.5
-
optimal stability, at 45°C-55°C
37372
11
-
37°C, 1 h, stable
37372
3 - 10
4 - 10
at 4°C
702760
4 - 10.4
purified recombinant enzyme, about 50% activity within this range
730852
4 - 11
4 - 12
5
-
30% loss of activity after 3 h, 90% loss of activity after 24 h
666570
5 - 10
-
2°C, 72 h, stable
37369
5 - 11
6 - 11
4 h, 30°C, more than 80% resdidual activity
749203
6 - 8
6 - 9
-
stable at 2°C for 72 h or at 30°C for 3 h
37367
6 - 9.5
-
stable
37382
6.5 - 11
-
40°C, stable
747718
6.5 - 9.5
-
37°C, 2 h
37388
7 - 10
-
purified recombinant His-tagged enzyme, 1 h, 60°C, without substrate, 30% activity remaining, at 37-50°C over 80% activity remain
730134
7 - 11.5
-
purified recombinant enzyme, 2 h, stable up to pH 9.0, inactivation at pH 11.5
729460
7 - 8.5
8.2 - 9.8
-
-
706768
8.5 - 11
recombinant enzyme shows a wider pH and thermal stability spectrum than the purified pectate lyase from Bacillus subtilis WSHB04-02
690786
9 - 10
9 - 12
9.4
-
45°C, stable for 300 h
678886
9.5
-
optimal stability, at 65°C
37372
9.7
-
45°C, about 30% loss of activity after 300 h
678886