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Literature summary for 4.2.2.2 extracted from

  • Miyazaki, Y.
    Purification and characterization of endo-pectate lyase from Bacillus macerans (1991), Agric. Biol. Chem., 55, 25-30.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
Paenibacillus macerans
EDTA
-
Paenibacillus macerans
Zn2+
-
Paenibacillus macerans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Km for polypectate: 0.11% Paenibacillus macerans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Paenibacillus macerans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Paenibacillus macerans
Ca2+ enhances activity 5fold Paenibacillus macerans
Mn2+ 1 mM, enhances activity 6fold Paenibacillus macerans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
1 * 35000, SDS-PAGE Paenibacillus macerans
45000
-
non-denaturing PAGE Paenibacillus macerans

Organism

Organism UniProt Comment Textmining
Paenibacillus macerans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Paenibacillus macerans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Paenibacillus macerans

Storage Stability

Storage Stability Organism
10°C, 100 mM ammonium chloride buffer, pH 9.0, stable for 1 month, 70% loss of activity in 20 mM ammonium chloride buffer, pH 9.0, after 24 h Paenibacillus macerans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin with 4.3% methoxyl groups Paenibacillus macerans ?
-
?
polygalacturonic acid
-
Paenibacillus macerans unsaturated oligogalacturonate
-
?
polypectate
-
Paenibacillus macerans ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 35000, SDS-PAGE Paenibacillus macerans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Paenibacillus macerans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 40°C: about 60% of maximal activity, 70°C: about 10% of maximal activity Paenibacillus macerans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
pH 6.5-9.6, 2 h, stable Paenibacillus macerans
50
-
10 min, stable in absence of Ca2+ Paenibacillus macerans
55
-
10 min, stable in presence of Ca2+ Paenibacillus macerans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
at 37°C Paenibacillus macerans

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 9.5 37°C, 2 h Paenibacillus macerans