Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Paenibacillus macerans | |
EDTA | - |
Paenibacillus macerans | |
Zn2+ | - |
Paenibacillus macerans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for polypectate: 0.11% | Paenibacillus macerans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Paenibacillus macerans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Paenibacillus macerans | |
Ca2+ | enhances activity 5fold | Paenibacillus macerans | |
Mn2+ | 1 mM, enhances activity 6fold | Paenibacillus macerans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 35000, SDS-PAGE | Paenibacillus macerans |
45000 | - |
non-denaturing PAGE | Paenibacillus macerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus macerans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Paenibacillus macerans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Paenibacillus macerans |
Storage Stability | Organism |
---|---|
10°C, 100 mM ammonium chloride buffer, pH 9.0, stable for 1 month, 70% loss of activity in 20 mM ammonium chloride buffer, pH 9.0, after 24 h | Paenibacillus macerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin | with 4.3% methoxyl groups | Paenibacillus macerans | ? | - |
? | |
polygalacturonic acid | - |
Paenibacillus macerans | unsaturated oligogalacturonate | - |
? | |
polypectate | - |
Paenibacillus macerans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, SDS-PAGE | Paenibacillus macerans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Paenibacillus macerans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | 40°C: about 60% of maximal activity, 70°C: about 10% of maximal activity | Paenibacillus macerans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
pH 6.5-9.6, 2 h, stable | Paenibacillus macerans |
50 | - |
10 min, stable in absence of Ca2+ | Paenibacillus macerans |
55 | - |
10 min, stable in presence of Ca2+ | Paenibacillus macerans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
at 37°C | Paenibacillus macerans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | 37°C, 2 h | Paenibacillus macerans |