Literature summary for 4.2.2.2 extracted from

Hassan, S.; Shevchik, V.E.; Robert, X.; Hugouvieux-Cotte-Pattat, N.
PelN is a new pectate lyase of Dickeya dadantii with unusual characteristics (2013), J. Bacteriol., 195, 2197-2206.

Search for more literature for 4.2.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
gene pelN, overexpression in Escherichia coli strain BL21(DE3) in the periplasmic space from pNA13	Dickeya dadantii

General Stability

General Stability	Organism
PelN stability is not at all, or only weakly, increased by the addition of Fe2+ or polygalacturonate	Dickeya dadantii

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	complete inhibition	Dickeya dadantii
additional information	PelN is only weakly affected by the degree of pectin methyl esterification	Dickeya dadantii

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	the enzyme is secreted by the Out system	Dickeya dadantii	-	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	required for activity, optimal at 0.1 to 0.3mM	Dickeya dadantii
additional information	no activation by Ca2+, Co2+, Cu2+, Mg2+, Mn2+, Ni2+, Zn2+, or Ba2+,	Dickeya dadantii

Organism

Organism	UniProt	Comment	Textmining
Dickeya dadantii	-	gene pelN	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	PelN acts synergistically with other pectine lyases in the organism, activity comparisons, overview	Dickeya dadantii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	PelN acts synergistically with other pectate lyases in the organism, activity comparisons, overview	Dickeya dadantii	?	-	?

Synonyms

Synonyms	Comment	Organism
PelN	-	Dickeya dadantii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Dickeya dadantii

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	recombinant enzyme, 90 min, 50% activity remaining	Dickeya dadantii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	-	Dickeya dadantii

Expression

Organism	Comment	Expression
Dickeya dadantii	the pelN expression is comtrolled by the repressors KdgR and PecS and is affected by different environmental conditions, such as pH, osmolarity, and temperature	down
Dickeya dadantii	the enzyme expression is controlled and activated by activator GacA	up

General Information

General Information	Comment	Organism
evolution	Dickeya dadantii pectate lyases belong to different families, namely, PL1, PL2, PL3, and PL9, PelN belongs to the PL9 family. The PelN orthologue is encoded by all of the sequenced genomes of the Dickeya and Pectobacterium species. The PelN structural model, constructed on the basis of the PelL structure, suggests that the PelL global topology and its catalytic amino acids are conserved in PelN. Notable differences concern the presence of additional loops at the PelN surface, and the replacement of PelL charged residues, involved in substrate binding, by aromatic residues in PelN	Dickeya dadantii
malfunction	no significant difference is detected after infection of potato tubers, but a weak decrease in the degree of maceration of chicory leaves is caused by the pelN mutant compared to the wild-type strain	Dickeya dadantii
additional information	the PelN structural model, constructed on the basis of the PelL structure, suggests that the PelL global topology and its catalytic amino acids are conserved in PelN. Notable differences concern the presence of additional loops at the PelN surface, and the replacement of PelL charged residues, involved in substrate binding, by aromatic residues in PelN	Dickeya dadantii

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Release 2023.1 (January 2023)