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4.2.2.2: pectate lyase

This is an abbreviated version!
For detailed information about pectate lyase, go to the full flat file.

Word Map on EC 4.2.2.2

Reaction

[pectate]n
=
4-deoxy-alpha-D-gluc-4-enuronosyl-1,4-[pectate]n-1
+
Alpha-D-glucuronic acid

Synonyms

alkaline pectate lyase, alkaline polygalacturonate lyase, alpha-1,4-D-endopolygalacturonic acid lyase, Apel, Athe_1854, AtPLL, Bsp165PelA, BxPEL1, BxPEL2, C0W65_13990, Calkro_0863, Cthe_2179, DKS1 pectate lyase, EC 4.2.99.3, endo-alpha-1,4-polygalacturonic acid lyase, endo-polygalacturonate trans-eliminase, endogalacturonate transeliminase, endopectin methyltranseliminase, FcPL1, Gr-PEL-2, Gr-PEL2, Hb-PEL-1, Hspel1, Hspel2, lyase, pectate, MdPL1, More, neutral-alkaline pectate lyase, NPLase, PATE, PCPEL2, pectate lyase, pectate lyase 10A, pectate lyase 2, pectate lyase 9A, pectate lyase A, pectate lyase B, pectate lyase C, pectate lyase E, pectate lyase I, pectate lyase L, pectate lyase Pel10A, pectate lyase Pel9A, pectate lyase PL 47, pectate transeliminase, pectic acid lyase, pectic acid transeliminase, pectic lyase, Pel, Pel I, Pel II, Pel III, Pel SWU, Pel-15, Pel-15E, Pel-15H, pel-2, Pel-20, Pel-22, Pel-66, Pel-90, Pel-BL11, Pel1, Pel10A, PEL168, Pel9A, PelA, PelB, pelC, PelD, PelE, PelI, PelN, PGA lyase, PGL, PL, PL D, PL I, PL-3, PL-STR, PL1B, Pla, PLB, PLC, PLE, poly(1,4-alpha-D-galacturonide) lyase, poly-galacturonic acid trans-eliminase, polygalacturonate lyase, polygalacturonic acid lyase, polygalacturonic transeliminase, PPase-N, ScPL NP_626147, ScPL NP_627050, XcPL NP_636037, XcPL NP_638163

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.2 pectate lyase

Molecular Weight

Molecular Weight on EC 4.2.2.2 - pectate lyase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10000000
-
matrix-assisted laser desorption ionization mass spectrometry
10600
-
ultracentrifugation
11000
-
gel filtration
12100
-
SDS-PAGE
133297
x * 133297, recombinant full-length enzyme, SDS-PAGE
135000
-
1 * 135000, pectate lyase a, SDS-PAGE
135171
x * 135171, calculated from sequence
148000
-
pectate lyase a, non-denaturing PAGE
158000
-
1 * 93000 + 1 * 158000, pectate lyase b, SDS-PAGE
20300
-
analytical ultracentrifugation
23000
23900
mature enzyme, electrospray ionization mass spectrometry
23960
mature enzyme, calculated from amino acid sequence
24700
x * 24700, Hspel2, calculated from sequence
26000
-
x * 26000, SDS-PAGE
26020
x * 26020, Hspel1, calculated from sequence
28000
30000
-
Pel II, gel filtration
30200
-
1 * 30200, Pel II, SDS-PAGE
31000
32000
-
gel filtration
33000
33355
-
x * 33355, calculation from nucleotide sequence
33400
-
SDS-PAGE
33450
calculated from amino acid sequence
33500
-
x * 33500, about, sequence calculation, x * 35000, recombinant enzyme, SDS-PAGE
34000
-
gel filtration
35000
35200
mature protein, predicted from cDNA
35500
-
Pel III, gel filtration
35940
calculated from amino acid sequence
36000 - 38000
Erwinia aroidea
-
gel filtration
36300
-
1 * 36300, Pel III, SDS-PAGE
37300
37482
-
x * 37482, pectate lyase B, calculation from nucleotide sequence
37500
-
x * 37500, enzyme from Alpine strain A15, SDS-PAGE
37556
x * 37556, sequence calculation
37700
mature protein, predicted from cDNA
38000
38500
-
x * 38500, SDS-PAGE
38600
-
x * 45498, sequence calculation, x * 38600, mature recombinant enzyme, SDS-PAGE
39000
39500
40000
41000
-
x * 41000, SDS-PAGE
41700
-
1 * 41700, Pel I, SDS-PAGE
42000
42500
42600
-
Pel I, gel filtration
43000
43640
deduced from cDNA
43900
predicted from cDNA
44000
44300
mature protein, predicted from cDNA
44400
-
SDS-PAGE
45000
45498
-
x * 45498, sequence calculation, x * 38600, mature recombinant enzyme, SDS-PAGE
46000
48000
-
x * 48000, SDS-PAGE
49900
mature protein, predicted from cDNA
50000
55000
-
x * 55000, SDS-PAGE
56000
-
SDS-PAGE
58513
x * 58513, recombinantly expressed catalytic module CM9-1, SDS-PAGE
68500
-
SDS-PAGE
70000
1 * 70000, SDS-PAGE
74000
-
x * 74000, SDS-PAGE
75000
-
gel filtration
76383
x * 76383, recombinantly expressed catalytic module CM9-2, SDS-PAGE
93000
-
1 * 93000 + 1 * 158000, pectate lyase b, SDS-PAGE
additional information
-
pectate lyase b shows two bands in non-denaturing PAGE: 200000 Da and 252000 Da