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4.2.2.2: pectate lyase

This is an abbreviated version!
For detailed information about pectate lyase, go to the full flat file.

Word Map on EC 4.2.2.2

Reaction

[pectate]n
=
4-deoxy-alpha-D-gluc-4-enuronosyl-1,4-[pectate]n-1
+
Alpha-D-glucuronic acid

Synonyms

alkaline pectate lyase, alkaline polygalacturonate lyase, alpha-1,4-D-endopolygalacturonic acid lyase, Apel, Athe_1854, AtPLL, Bsp165PelA, BxPEL1, BxPEL2, C0W65_13990, Calkro_0863, Cthe_2179, DKS1 pectate lyase, EC 4.2.99.3, endo-alpha-1,4-polygalacturonic acid lyase, endo-polygalacturonate trans-eliminase, endogalacturonate transeliminase, endopectin methyltranseliminase, FcPL1, Gr-PEL-2, Gr-PEL2, Hb-PEL-1, Hspel1, Hspel2, lyase, pectate, MdPL1, More, neutral-alkaline pectate lyase, NPLase, PATE, PCPEL2, pectate lyase, pectate lyase 10A, pectate lyase 2, pectate lyase 9A, pectate lyase A, pectate lyase B, pectate lyase C, pectate lyase E, pectate lyase I, pectate lyase L, pectate lyase Pel10A, pectate lyase Pel9A, pectate lyase PL 47, pectate transeliminase, pectic acid lyase, pectic acid transeliminase, pectic lyase, Pel, Pel I, Pel II, Pel III, Pel SWU, Pel-15, Pel-15E, Pel-15H, pel-2, Pel-20, Pel-22, Pel-66, Pel-90, Pel-BL11, Pel1, Pel10A, PEL168, Pel9A, PelA, PelB, pelC, PelD, PelE, PelI, PelN, PGA lyase, PGL, PL, PL D, PL I, PL-3, PL-STR, PL1B, Pla, PLB, PLC, PLE, poly(1,4-alpha-D-galacturonide) lyase, poly-galacturonic acid trans-eliminase, polygalacturonate lyase, polygalacturonic acid lyase, polygalacturonic transeliminase, PPase-N, ScPL NP_626147, ScPL NP_627050, XcPL NP_636037, XcPL NP_638163

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.2 pectate lyase

Temperature Stability

Temperature Stability on EC 4.2.2.2 - pectate lyase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
-
half-life 2 h
30 - 40
inactivation of recombinant PL I occurs above 30°C whereas the activity of native PL I is stable up to 40°C
30 - 60
PelA is stable during 30°C to 40°C and not very stable at 50°C (in 50 mM glycine-NaOH buffer, pH 10.0), about 60% activity is retained after 30 min incubation at 50°C and it almost completely loses activity when it is incubated at 60°C
35 - 45
-
optimal stability
37 - 50
-
purified recombinant His-tagged enzyme, 45 min, without substrate, pH 9.0-12.0, over 80% activity remaining
39
-
transition midpoint of pectate lyase C is 38.9°C
40 - 50
40 - 70
the enzyme loses 60% of its activity when incubated at 40°C for 1 h after treatment with EDTA, thermostability increases to approximately 80% of initial activity at 50 and 70°C in the presence of Ca2+ alone and both Ca2+ and polygalacturonic acid, respectively
44
-
transition midpoint of pectate lyase C is 44.3°C
45 - 50
purified recombinant PelN displays a half-life of around 9 h and 42 h at 50°C and 45°C, respectively
47.9
melting temperature, mutant N198Q
48.7
melting temperature, mutant N198D
48.9
melting temperature, mutant N198A
50.7
melting temperature, mutant N95S
55 - 70
more than 80% of the original enzyme activity is retained in Tris-HCl buffers with pH 7 and 8, after incubation for 30 min
57
-
20 min, complete inactivation
60 - 95
-
the enzyme keeps stable, possesses a high level of activity at 60°C and a half-life of almost 2 h at 95°C
74
melting temperature
86
melting temperature, presence of 0.6 mM Ca2+
additional information