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evolution
pectate lyase 2 sequence analysis and evolutionary analysis, overview
evolution
pectate lyase 2 sequence analysis and evolutionary analysis, overview
evolution
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pectate lyase 2 sequence analysis and evolutionary analysis, overview
evolution
pectate lyase 2 sequence analysis and evolutionary analysis, overview
evolution
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Dickeya dadantii pectate lyases belong to different families, namely, PL1, PL2, PL3, and PL9, PelN belongs to the PL9 family. The PelN orthologue is encoded by all of the sequenced genomes of the Dickeya and Pectobacterium species. The PelN structural model, constructed on the basis of the PelL structure, suggests that the PelL global topology and its catalytic amino acids are conserved in PelN. Notable differences concern the presence of additional loops at the PelN surface, and the replacement of PelL charged residues, involved in substrate binding, by aromatic residues in PelN
evolution
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parallel beta-helix, active site residues, and substrate binding cleft are similar to those in the other pectate lyases from polysaccharide lyase family 1, PL1
evolution
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PL-STR belongs to family PF00544
evolution
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the enzyme belongs to family PF09492
evolution
the enzyme belongs to pectate lyase family 3
evolution
the enzyme belongs to te pectate lyase family
evolution
the enzyme belongs to the polysaccharide lyase family 1
evolution
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the mature Apel is structurally related to the enzymes in the polysaccharide lyase family 1
evolution
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parallel beta-helix, active site residues, and substrate binding cleft are similar to those in the other pectate lyases from polysaccharide lyase family 1, PL1
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evolution
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the enzyme belongs to family PF09492
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evolution
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the mature Apel is structurally related to the enzymes in the polysaccharide lyase family 1
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malfunction
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construction of CcpelA gene-disrupted mutants, the mutants show reduced aggressiveness towards tomato fruits and impaired pectate lyase secretion and extracellular activity, while overexpression of CcpelA in the Si-60 isolate increases its aggressiveness and PL secretion, overview
malfunction
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type II secretion system-deficient mutant of Dickeya dadantii 3937, A1919, DELTA ouC, loses the capability to promote the multiplication of EDL933, whereas Ech159, DELTApoS, a stress-responsive sigma-factor RpoS-deficient mutant, increases EDL933 proliferation on lettuce leaves 2fold mor than the wild-type strain. Mutant A1919 is completely deficient in the secretion of pectate lyases, which play a major role in plant tissue maceration
malfunction
gene inactivation does not result in complete loss of pectate lyase activity, but the symptoms of anthracnose in the infected host plants are reduced
malfunction
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no significant difference is detected after infection of potato tubers, but a weak decrease in the degree of maceration of chicory leaves is caused by the pelN mutant compared to the wild-type strain
malfunction
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type II secretion system-deficient mutant of Dickeya dadantii 3937, A1919, DELTA ouC, loses the capability to promote the multiplication of EDL933, whereas Ech159, DELTApoS, a stress-responsive sigma-factor RpoS-deficient mutant, increases EDL933 proliferation on lettuce leaves 2fold mor than the wild-type strain. Mutant A1919 is completely deficient in the secretion of pectate lyases, which play a major role in plant tissue maceration
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physiological function
PEL may play an important role in the process of normal fiber elongation in cotton
physiological function
pectate lyase 2 degrades the unesterified polygalacturonate pectate of the host cell wall
physiological function
pectate lyase 2 degrades the unesterified polygalacturonate pectate of the host cell wall
physiological function
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pectate lyase 2 degrades the unesterified polygalacturonate pectate of the host cell wall
physiological function
pectate lyase 2 degrades the unesterified polygalacturonate pectate of the host cell wall
physiological function
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pectate lyase is involved in cell wall hydrolysis and pulp softening during ripening fruits
physiological function
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role of secreted pectate lyase, a cell wall-degrading enzyme, in the aggressiveness of Colletotrichum coccodes, overview
physiological function
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the influence of the virulence mechanisms of Dickeya dadantii strain 3937, a broad-host-range phytopathogen, on the proliferation of the human pathogen Escherichia coli O157:H7 EDL933 on postharvest lettuce, strain 3937 promotes the multiplication of EDL933, overview
physiological function
the organism causes anthracnose in infected bean plants, pectate lyase encoded by the pecCl1 gene is an important determinant for the aggressiveness of Colletotrichum lindemuthianum
physiological function
activity is lost at low pH because protonation of aspartates results in the loss of the two catalytic calcium-ions causing a profound failure to correctly organise the Michaelis complex
physiological function
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analysis of 30 pectate lyase-like genes in Populus trichocarpa. Most of the PL1 genes from subgroups Ia and Ib are highly expressed in xylem. Isoform PL1-18 from subgroup Ia is preferentially expressed in developing primary xylem and in xylem cells that are developing secondary walls. Overexpression of PL1-18 in poplar reduces plant growth and xylem development. Reduced secondary cell wall thickening and irregular xylem cells are observed in the transgenic trees, probably due to their lower pectin content
physiological function
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downregulation of pectate lyase by antisense transformation leads to transgenicplants with fruits firmer than the control. The average molecular masses of transgenic pectins are higher than that of the control. The mean length values for chelated chains increases from 84 nm in the control to 95.5 nm in antisense samples. Sodium carbonate-soluble polyuronides are longer in transgenic fruits. Transgenic pectins show a more complex structure, with a higher percentage of branched chains than the control
physiological function
purified Pel1 triggers defense responses and confers resistance to Botrytis cinerea and Verticillium dahliae in tobacco and cotton plants. A mutant proterin lacking the enzymatic activity lacks functions to induce both cell death and plant resistance. Enzyme deletion strains severely compromise the virulence of Verticillium dahliae
physiological function
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activity is lost at low pH because protonation of aspartates results in the loss of the two catalytic calcium-ions causing a profound failure to correctly organise the Michaelis complex
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physiological function
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pectate lyase is involved in cell wall hydrolysis and pulp softening during ripening fruits
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physiological function
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purified Pel1 triggers defense responses and confers resistance to Botrytis cinerea and Verticillium dahliae in tobacco and cotton plants. A mutant proterin lacking the enzymatic activity lacks functions to induce both cell death and plant resistance. Enzyme deletion strains severely compromise the virulence of Verticillium dahliae
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physiological function
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the influence of the virulence mechanisms of Dickeya dadantii strain 3937, a broad-host-range phytopathogen, on the proliferation of the human pathogen Escherichia coli O157:H7 EDL933 on postharvest lettuce, strain 3937 promotes the multiplication of EDL933, overview
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additional information
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implication of Bacillus sp. in the production of pectinolytic enzymes during cocoa fermentation
additional information
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implication of Bacillus sp. in the production of pectinolytic enzymes during cocoa fermentation
additional information
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implication of Bacillus sp. in the production of pectinolytic enzymes during cocoa fermentation
additional information
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implication of Bacillus sp. in the production of pectinolytic enzymes during cocoa fermentation
additional information
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implication of Bacillus sp. in the production of pectinolytic enzymes during cocoa fermentation
additional information
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implication of Bacillus sp. in the production of pectinolytic enzymes during cocoa fermentation
additional information
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RpoS, the general stress response sigma-factor involved in cell survival in suboptimal conditions, plays a role in EDL933 proliferation by controlling the production of pectate lyases in Dickeya dadantii 3937, e.g. via negative regulation of pelD promoter activity
additional information
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the organism is the soft rot pathogen of calla lily growing around Kunming, soft rot is a major disease of calla lily, Zantedeschia spp., and other important crops worldwide. The pectate lyase contributes to the disease by tuber maceration cleaving structural pectic polymers, overview
additional information
invariant amino acids involved in catalytic function mainly comprised the catalytic residues R275, K244, and R280, and the Ca2+-binding residues D151, D173, and D177 in PelN
additional information
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Pel structure modeling, overview
additional information
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the enzyme from Xanthomonas campestris ACCC 10048 is a low-temperature-active alkaline pectate lyase
additional information
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the PelN structural model, constructed on the basis of the PelL structure, suggests that the PelL global topology and its catalytic amino acids are conserved in PelN. Notable differences concern the presence of additional loops at the PelN surface, and the replacement of PelL charged residues, involved in substrate binding, by aromatic residues in PelN
additional information
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the enzyme from Xanthomonas campestris ACCC 10048 is a low-temperature-active alkaline pectate lyase
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additional information
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Pel structure modeling, overview
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additional information
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the organism is the soft rot pathogen of calla lily growing around Kunming, soft rot is a major disease of calla lily, Zantedeschia spp., and other important crops worldwide. The pectate lyase contributes to the disease by tuber maceration cleaving structural pectic polymers, overview
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additional information
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RpoS, the general stress response sigma-factor involved in cell survival in suboptimal conditions, plays a role in EDL933 proliferation by controlling the production of pectate lyases in Dickeya dadantii 3937, e.g. via negative regulation of pelD promoter activity
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