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4.2.2.2: pectate lyase

This is an abbreviated version!
For detailed information about pectate lyase, go to the full flat file.

Word Map on EC 4.2.2.2

Reaction

[pectate]n
=
4-deoxy-alpha-D-gluc-4-enuronosyl-1,4-[pectate]n-1
 +
Alpha-D-glucuronic acid

Synonyms

alkaline pectate lyase, alkaline polygalacturonate lyase, alpha-1,4-D-endopolygalacturonic acid lyase, Apel, Athe_1854, AtPLL, Bsp165PelA, BxPEL1, BxPEL2, C0W65_13990, Calkro_0863, Cthe_2179, DKS1 pectate lyase, EC 4.2.99.3, endo-alpha-1,4-polygalacturonic acid lyase, endo-polygalacturonate trans-eliminase, endogalacturonate transeliminase, endopectin methyltranseliminase, FcPL1, Gr-PEL-2, Gr-PEL2, Hb-PEL-1, Hspel1, Hspel2, lyase, pectate, MdPL1, More, neutral-alkaline pectate lyase, NPLase, PATE, PCPEL2, pectate lyase, pectate lyase 10A, pectate lyase 2, pectate lyase 9A, pectate lyase A, pectate lyase B, pectate lyase C, pectate lyase E, pectate lyase I, pectate lyase L, pectate lyase Pel10A, pectate lyase Pel9A, pectate lyase PL 47, pectate transeliminase, pectic acid lyase, pectic acid transeliminase, pectic lyase, Pel, Pel I, Pel II, Pel III, Pel SWU, Pel-15, Pel-15E, Pel-15H, pel-2, Pel-20, Pel-22, Pel-66, Pel-90, Pel-BL11, Pel1, Pel10A, PEL168, Pel9A, PelA, PelB, pelC, PelD, PelE, PelI, PelN, PGA lyase, PGL, PL, PL D, PL I, PL-3, PL-STR, PL1B, Pla, PLB, PLC, PLE, poly(1,4-alpha-D-galacturonide) lyase, poly-galacturonic acid trans-eliminase, polygalacturonate lyase, polygalacturonic acid lyase, polygalacturonic transeliminase, PPase-N, ScPL NP_626147, ScPL NP_627050, XcPL NP_636037, XcPL NP_638163

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.2 pectate lyase

Purification

Purification on EC 4.2.2.2 - pectate lyase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
38000 Da N-terminally His-tagged Pel10Acm
-
5 isoenzymes: PelA, PelB, PelC, PelD, PelE
-
6*His-tagged recombinant enzyme
affinity column chromatography and gel filtration
-
an extracellular pectate lyase is purified from the culture filtrate of a newly isolated Bacillus pumilus DKS1 grown in pectin containing medium using ion-exchange and gel filtration chromatography
-
CM-Toyopearl 650M column chromatography, Mono S column chromatography, Resource PHE column chromatography, and ammonium sulfate precipitation, recombinant enzyme is purified by amylase resin column chromatography
DEAE-Sepharose column chromatography
ion exchange chromatography and gel filtration
isoenzyme PL I and PL II
-
native enzyme 18.6fold from strain AK2 culture supernatant by cation exchange chromatography and gel filtration
-
native extracellular Pel1 71fold by anion exchange chromatography
-
native Pel-22 from supernatant by ammonium sulfate fractionation and cation exchange chromatography
native Pel-66 from supernatant by ammonium sulfate fractionation and cation exchange chromatography
native Pel-90 from supernatant by ammonium sulfate fractionation and cation exchange chromatography
Ni-NTA column chromatography
-
Ni-NTA column chromatography, gel filtration
Ni-NTA-agarose column chromatography
pectate lyase a and pectate lyase b
-
pectate lyase B
-
pectate lyase C
-
pectate lyase D
-
pectate lyase I and II
-
pectate lyase I and pectate lyase II
-
pectate lyase III
-
purification by anion exchange chromatography, followed by heat treatment of the enzyme solution at 60°C for 30 min, and a gel filtration
-
purification of the recombinant enzyme results in identification of two enzyme forms of which one appears to be N-glycosylated and the other appears to be free of N-glycosylation
recombinant
recombinant enzyme
recombinant enzyme 17.3fold from strain WB600 culture supernatant by ammonium sulfate fractionation, cation exchange chromatography, and gel filtration
-
recombinant enzyme from Escherichia coli strain BL21(DE3) plysS 6.64fold by single step cation exchange chromatography to homogeneity
recombinant enzyme PelN 3fold from Escherichia coli strain BL21(DE3)
recombinant His-tagged enzyme 2.47fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chrmatography to homogeneity
-
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatgraphy
-
recombinant His-tagged PCPEL2 from Escherichia coli strain BL21(DE3), 34.1fold from the pLysS system and 53.8fold from the pMal system, by nickel affinity chromatography and gel filtration chromatography, recombinant MBP-PCPEL2 by amylose affinity chromatography
recombinant His-tagged Pel-BL11 from Escherichia coli by nickel affinity and anion exchange chromatography
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, gel filtration, and ultrafiltration
recombinant protein, both from soluble fraction and from inclusion bodies
single-step
-
using butyl-Toyopearl 650 column
wild-type and mutant enzymes
-