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4.1.1.1: pyruvate decarboxylase

This is an abbreviated version!
For detailed information about pyruvate decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.1

Reaction

a 2-oxo carboxylate
=
an aldehyde
 +
CO2

Synonyms

2-oxo acid carboxylyase, 8-10 nm cytoplasmic filament-associated protein, acetohydroxyacid synthase, AHAS, alpha-Carboxylase, alpha-Keto acid carboxylase, ANC27, bifunctional pyruvate decarboxylase/pyruvate ferredoxin oxidoreductase, Decarboxylase, pyruvate, GPDC1, IFPL730, KivD, KlPDC, KviD, NcPDC, P59NC, PDC, PDC I, PDC II, PDC1, Pdc1p, PDC2, PDC3, PDC4, Pdc5, Pdc5p, Pdc6, PDCS.c., PDCZ.m., PDHC, POR, pyruvamide-activated yeast pyruvate decarboxylase, pyruvate decarboxylase, pyruvate decarboxylase 1, pyruvate decarboxylase 2, pyruvate decarboxylase complex, Pyruvic decarboxylase, R-selective pyruvate decarboxylase, SCPDC, scpdc1, ScPDC5, TTHA1213, yeast pyruvate decarboxylase, YPDC, ZbPDC, ZmPDC

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.1 pyruvate decarboxylase

Purification

Purification on EC 4.1.1.1 - pyruvate decarboxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
136fold, recombinant PDC, expressed in Escherichia coli
2 isoenzymes: I and II
-
5 C-terminal deletion mutants
DEAE column chromatography and HAP column chromatography
-
Fractogel EMD TMAE column chromatography and Superdex 200 gel filtration
-
His SpinTrap column chromatography
-
HisBind resin column chromatography
-
HisTrap column chromatography and Superdex 200 gel filtration
HisTrap column chromatography, Fractogel EMD TMAE 650S gel filtration, and Superdex 200 gel filtration
-
HiTrap chelating column chromatography
-
HiTrap Ni2+ chelating HP column chromatography
-
MBPTrap column chromatography
-
native enzyme by acetone precipitation, ammonium sulfate fractionation, and gel filtration to over 95% homogeneity
native enzyme by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography to over 95% homogeneity
native enzyme partially
-
native isozyme 17fold by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography
native isozyme 22fold by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography
partial
partial purification by acetone precipitation
-
partially purified, ammonium sulfate precipitation and phenyl Sepharose column chromatography
-
PDC mutants D27E, D27N, E473D and E473Q
-
purified enzyme shos a brownish colour characteristic of iron-sulfur containing proteins
Q56317; 05650; Q56316; O05651
purified enzyme shows a brownish colour characteristic of iron-sulfur containing proteins
recombinant enzyme mutants from Escherichia coli strain BL21(DE3)
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant homomeric, alpha-only, PDC1
-
recombinant mutants D28A and His6-tagged E477Q, the latter on a talon resin
-
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3)
-
W412F and W412A mutant PDC, expressed in Escherichia coli, W412A is purified as apoenzyme
-
wild-type and D28A, D28N, E477Q mutant YPDC
-
wild-type PDC and C221A/C222A double mutant
-
wild-type, C221E/C222A and C221A/C222A double mutant YPDC
-
wild-type, E91A, E91D and E91Q mutant PDC, expressed in Escherichia coli, mutants are purified as apoenzymes
-