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4.1.1.1: pyruvate decarboxylase

This is an abbreviated version!
For detailed information about pyruvate decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.1

Reaction

a 2-oxo carboxylate
=
an aldehyde
 +
CO2

Synonyms

2-oxo acid carboxylyase, 8-10 nm cytoplasmic filament-associated protein, acetohydroxyacid synthase, AHAS, alpha-Carboxylase, alpha-Keto acid carboxylase, ANC27, bifunctional pyruvate decarboxylase/pyruvate ferredoxin oxidoreductase, Decarboxylase, pyruvate, GPDC1, IFPL730, KivD, KlPDC, KviD, NcPDC, P59NC, PDC, PDC I, PDC II, PDC1, Pdc1p, PDC2, PDC3, PDC4, Pdc5, Pdc5p, Pdc6, PDCS.c., PDCZ.m., PDHC, POR, pyruvamide-activated yeast pyruvate decarboxylase, pyruvate decarboxylase, pyruvate decarboxylase 1, pyruvate decarboxylase 2, pyruvate decarboxylase complex, Pyruvic decarboxylase, R-selective pyruvate decarboxylase, SCPDC, scpdc1, ScPDC5, TTHA1213, yeast pyruvate decarboxylase, YPDC, ZbPDC, ZmPDC

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.1 pyruvate decarboxylase

Storage Stability

Storage Stability on EC 4.1.1.1 - pyruvate decarboxylase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C or -80°C, pH 6.0, 1 mM thiamine diphosphate, 1 mM MgSO4, minimum protein concentration 0.2 mg/ml, stable for several months
-
-20°C, 50% ethylene glycol, stable for 3 months
-
-20°C, 50% v/v glycerol, negligible loss of activity
-
-20°C, concentrated PDC, buffered solution containing thiamine diphosphate and MgSO4, several months, stable
-20°C, little or no loss of activity after several months
-
-20°C, lyophilized preparation, stable for at least 3 months
-70°C, 10 mM PIPES at pH 6.5 with 1 mM dithiothreitol, 1 mM MgCl2, and 0.1 mM thiamine diphosphate, several weeks, no loss of activity
-
4°C, wild-type and W412F mutant PDC, several months, stable
-
half-life of 3 days for crude enzyme extract and less than 1 day for whole cell biomass in absence or presence of benzaldehyde at 22°C
-