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Literature summary for 4.1.1.1 extracted from

  • Meyer, D.; Neumann, P.; Parthier, C.; Friedemann, R.; Nemeria, N.; Jordan, F.; Tittmann, K.
    Double duty for a conserved glutamate in pyruvate decarboxylase: evidence of the participation in stereoelectronically controlled decarboxylation and in protonation of the nascent carbanion/enamine intermediate (2010), Biochemistry, 49, 8197-8212.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
wild type enzyme is expressed in Escherichia coli SG13009 cells, mutant enzymes E473D and E473Q are expressed in Escherichia coli JM109 cells Zymomonas mobilis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 100 mM MES buffer (pH 6.0), 1 mM thiamin diphosphate, 5 mM MgSO4, and 20-24% (w/v) polyethylene glycol 1500 Zymomonas mobilis

Protein Variants

Protein Variants Comment Organism
E473D the mutant exhibits a residual activity of 0.6% compared to the wild type enzyme, wild type PDC and the Glu473Asp variant bind the substrate analogue acetylphosphinate with the same affinity Zymomonas mobilis
E473Q the mutant exhibits a residual activity of 0.1% compared to the wild type enzyme, Glu473Gln fails to bind the substrate analogue acetylphosphinate Zymomonas mobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 pyruvate mutant enzyme E473D, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate Zymomonas mobilis
0.31
-
 pyruvate wild type enzyme, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate Zymomonas mobilis
0.4
-
 pyruvate mutant enzyme E473Q, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate Zymomonas mobilis

Organism

Organism UniProt Comment Textmining
Zymomonas mobilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Fractogel EMD TMAE column chromatography and Superdex 200 gel filtration Zymomonas mobilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetylphosphinate
-
 Zymomonas mobilis ?
-
 ?
pyruvate
-
 Zymomonas mobilis acetaldehyde + CO2
-
 ?

Synonyms

Synonyms Comment Organism
PDC
-
 Zymomonas mobilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 pyruvate mutant enzyme E473D, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate Zymomonas mobilis
0.15
-
 pyruvate mutant enzyme E473Q, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate Zymomonas mobilis
150
-
 pyruvate wild type enzyme, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate Zymomonas mobilis

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
 Zymomonas mobilis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.38
-
 pyruvate mutant enzyme E473Q, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate Zymomonas mobilis
0.67
-
 pyruvate mutant enzyme E473D, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate Zymomonas mobilis
484
-
 pyruvate wild type enzyme, at 30°C in 50 mM MES buffer (pH 6.0) containing 1 mM MgSO4 and 0.1 mM thiamine diphosphate Zymomonas mobilis