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Literature summary for 4.1.1.1 extracted from

  • Lu, G.; Dobritzsch, D.; Baumann, S.; Schneider, G.; Konig, S.
    The structural basis of substrate activation in yeast pyruvate decarboxylase. A crystallographic and kinetic study (2000), Eur. J. Biochem., 267, 861-868.
    View publication on PubMed
Search for more literature for 4.1.1.1

Activating Compound

Activating Compound Comment Organism Structure
Pyruvamide activator, 2 binding sites per dimer, mode of binding, a disorder-order transition of two active-site loop regions is a key event in the activation process, kinetic data, mechanism Saccharomyces cerevisiae
pyruvate substrate activation, mechanism Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
PDC complex with pyruvamide Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ cofactor Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate Saccharomyces cerevisiae penultimate step in the alcoholic fermentation process acetaldehyde + CO2
-
 ?
pyruvate Saccharomyces cerevisiae WS34/70 penultimate step in the alcoholic fermentation process acetaldehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae WS34/70
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
43 46 pH 6, 30°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate 4 active sites in the tetramer, enzyme structure Saccharomyces cerevisiae acetaldehyde + CO2
-
 ?
pyruvate penultimate step in the alcoholic fermentation process Saccharomyces cerevisiae acetaldehyde + CO2
-
 ?
pyruvate 4 active sites in the tetramer, enzyme structure Saccharomyces cerevisiae WS34/70 acetaldehyde + CO2
-
 ?
pyruvate penultimate step in the alcoholic fermentation process Saccharomyces cerevisiae WS34/70 acetaldehyde + CO2
-
 ?

Subunits

Subunits Comment Organism
tetramer enzyme structure, differences in the tetramer assembly of form A and B PDC, form A is the native PDC Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate requirement Saccharomyces cerevisiae