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Literature summary for 4.1.1.1 extracted from

  • Spinka, M.; Seiferheld, S.; Zimmermann, P.; Bergner, E.; Blume, A.K.; Schierhorn, A.; Reichenbach, T.; Pertermann, R.; Ehrt, C.; Koenig, S.
    Significance of individual residues at the regulatory site of yeast pyruvate decarboxylase for allosteric substrate activation (2017), Biochemistry, 56, 1285-1298.
    View publication on PubMed
Search for more literature for 4.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
A287G the mutant shows reduced activity compared to the wild type enzyme Saccharomyces cerevisiae
C221A/C222A the mutant shows reduced activity compared to the wild type enzyme Saccharomyces cerevisiae
H225F the mutant shows reduced activity compared to the wild type enzyme Saccharomyces cerevisiae
H310F the mutant shows reduced activity compared to the wild type enzyme Saccharomyces cerevisiae
H92F the mutant shows wild type activity Saccharomyces cerevisiae
S311A the mutant shows reduced activity compared to the wild type enzyme Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 pyruvate mutant enzyme A287G, at pH 6.0 and 30°C Saccharomyces cerevisiae
0.1
-
 pyruvate mutant enzyme S311A, at pH 6.0 and 30°C Saccharomyces cerevisiae
0.3
-
 pyruvate mutant enzyme H92F, at pH 6.0 and 30°C Saccharomyces cerevisiae
0.4
-
 pyruvate mutant enzyme C221A/C222A, at pH 6.5 and 10°C Saccharomyces cerevisiae
0.5
-
 pyruvate wild type enzyme, at pH 6.0 and 30°C Saccharomyces cerevisiae
16
-
 pyruvate mutant enzyme H310F, at pH 6.0 and 30°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate Saccharomyces cerevisiae
-
 acetaldehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography, Fractogel EMD TMAE 650S gel filtration, and Superdex 200 gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
 Saccharomyces cerevisiae acetaldehyde + CO2
-
 ?

Synonyms

Synonyms Comment Organism
2-oxo acid carboxylyase
-
 Saccharomyces cerevisiae
PDC
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate dependent on Saccharomyces cerevisiae