3.5.1.2: glutaminase
This is an abbreviated version!
For detailed information about glutaminase, go to the full flat file.
Word Map on EC 3.5.1.2
-
3.5.1.2
-
ammonia
-
phosphate-dependent
-
glutaminolysis
-
neurotransmitter
-
glutamatergic
-
l-asparaginase
-
amidotransferase
-
cerebral
-
astrocyte
-
asparagine
-
excitatory
-
ammoniagenesis
-
glutamine-dependent
-
6-diazo-5-oxo-l-norleucine
-
hexokinase
-
tricarboxylic
-
tca
-
alpha-ketoglutarate
-
lymphoblastic
-
phosphoenolpyruvate
-
deamidation
-
gamma-glutamyl
-
carboxykinase
-
synaptosomes
-
bicarbonate
-
neurotransmission
-
enterocytes
-
addict
-
gabaergic
-
transamination
-
transmitter
-
l-glutamate
-
acidotic
-
hyperammonemia
-
nh3
-
carbamyl
-
warburg
-
ammoniagenic
-
anaplerosis
-
aminooxyacetate
-
alkalosis
-
nh4cl
-
acivicin
-
glutamate-glutamine
-
perivenous
-
slc1a5
-
chrysanthemi
-
carbamoylphosphate
-
ureagenesis
-
analysis
-
medicine
-
nutrition
-
synthesis
-
food industry
-
glucosamine-6-phosphate
- 3.5.1.2
- ammonia
-
phosphate-dependent
-
glutaminolysis
-
neurotransmitter
-
glutamatergic
- l-asparaginase
-
amidotransferase
- cerebral
- astrocyte
- asparagine
-
excitatory
-
ammoniagenesis
-
glutamine-dependent
- 6-diazo-5-oxo-l-norleucine
- hexokinase
-
tricarboxylic
- tca
- alpha-ketoglutarate
- lymphoblastic
- phosphoenolpyruvate
-
deamidation
-
gamma-glutamyl
-
carboxykinase
-
synaptosomes
- bicarbonate
-
neurotransmission
- enterocytes
-
addict
-
gabaergic
-
transamination
-
transmitter
- l-glutamate
-
acidotic
- hyperammonemia
- nh3
-
carbamyl
-
warburg
-
ammoniagenic
-
anaplerosis
- aminooxyacetate
- alkalosis
- nh4cl
- acivicin
-
glutamate-glutamine
-
perivenous
-
slc1a5
- chrysanthemi
- carbamoylphosphate
-
ureagenesis
- analysis
- medicine
- nutrition
- synthesis
- food industry
- glucosamine-6-phosphate
Reaction
Synonyms
AnsB, AoGls, GA, GAB, GAC, GahB, GLA, GLNase, GLS, GLS1, GLS2, GlsA, glutaminase, glutaminase 1, glutaminase 2, glutaminase A, glutaminase B, glutaminase C, glutaminase I, glutaminase K, glutaminase L, glutaminase-1, glutaminase-2, glutaminase-B, glutamine amidohydrolase, glutamine aminohydrolase, glutamine deamidating enzyme, K-glutaminase, KAG, KGA, kidney-type glutaminase, kidney-type-glutaminase, L-glutaminase, L-glutamine amidohydrolase, LAG, LGA, liver-type glitaminase, liver-type glutaminase, Mglu, Micrococcus glutaminase Mglu, Micrococcus luteus K-3-type glutaminase, mitochondrial glutaminase, N-PAG, neuroblastoma glutaminase, neuroblastoma PAG, Nit 2, nitrilase 2, omega-amidase, PAG, PDX2, phosphate activated glutaminase, phosphate-activated glutaminase, phosphate-activated L-glutamine amidohydrolase, salt-tolerant glutaminase, YaaE
ECTree
3.5.1.2 glutaminaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.5.1.2 - glutaminase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
6-diazo-5-oxo-L-norleucine + H2O
?
the nucleophilic attack of Ser286 sidechain on 6-diazo-5-oxo-L-norleucine releases the diazo group (N2) from the inhibitor and results in the formation of an enzyme-inhibitor complex
-
-
?
beta-cyanoalanine + H2O
?
-
0.2% of the activity with L-Gln
-
-
?
D-Asn + H2O
D-aspartate + NH3
-
less 0.1% of the activity with L-glutamine and H2O as co-substrate
-
-
?
D-Asn + hydroxylamine
D-2-amino-4-(hydroxylamino)-4-oxobutanoic acid + NH3
-
-
-
-
?
D-gamma-glutamyl-ethylester + hydroxylamine
D-gamma-glutamyl hydroxamate + ethanol
-
-
-
-
?
D-gamma-glutamyl-hydrazide + hydroxylamine
D-gamma-glutamyl hydroxamate + hydroxyhydrazine
-
-
-
-
?
D-gamma-glutamyl-methylester + hydroxylamine
L-gamma-glutamyl hydroxamate + methanol
-
-
-
-
?
D-Gln + hydroxylamine
D-gamma-glutamyl hydroxamate + NH3
-
glutaminase A and B
-
?
gamma-ethyl glutamate + hydroxylamine
gamma-glutamyl hydroxamate + ethanol
-
-
-
-
?
gamma-glutamyl hydroxamate + NH2OH
?
-
36.3% of the activity with L-glutamine and H2O as co-substrate
-
-
?
gamma-glutamyl-hydroxamate + hydroxylamine
gamma-glutamyl hydroxamate + hydroxylamine
-
-
-
-
?
gamma-glutamyl-methoxyamide + H2O
Glu + N-hydroxy-O-methylhydroxylamine
-
-
-
-
?
gamma-glutamyl-methoxyamide + hydroxylamine
gamma-glutamyl hydroxamate + N-hydroxy-O-methylhydroxylamine
-
-
-
-
?
gamma-glutamyl-methylamide + hydroxylamine
gamma-glutamyl hydroxamate + N-methylhydroxylamine
-
-
-
-
?
gamma-L-glutamyl-4-nitroanilide + H2O
L-glutamate 4-nitroanilide + NH3
-
102% activity compared to L-glutamine
-
-
?
gamma-methyl glutamate + hydroxylamine
gamma-glutamyl hydroxamate + methanol
-
-
-
-
?
gamma-thioethyl glutamate + hydroxylamine
gamma-glutamyl hydroxamate + ?
-
-
-
-
?
gamma-thiomethyl glutamate + hydroxylamine
gamma-glutamyl hydroxamate + ?
-
-
-
-
?
L-Asn + H2O
L-aspartate + NH3
-
20.6% of the activity with L-glutamine and H2O as co-substrate
-
-
?
L-Asn + hydroxylamine
L-2-amino-4-(hydroxylamino)-4-oxobutanoic acid + NH3
-
-
-
-
?
L-gamma-glutamyl-anilide + hydroxylamine
L-gamma-glutamyl hydroxamate + aniline
-
-
-
-
?
L-gamma-glutamyl-hydrazide + hydroxylamine
L-gamma-glutamyl hydroxamate + hydroxyhydrazine
-
-
-
-
?
L-gamma-glutamyl-L-Ala + hydroxylamine
gamma-glutamyl hydroxamate + Ala
-
-
-
-
?
L-gamma-glutamyl-methylester + hydroxylamine
L-gamma-glutamyl hydroxamate + methanol
-
-
-
-
?
L-glutamic acid + NH2OH
gamma-glutamyl hydroxamate
-
0.9% of the activity with L-glutamine and H2O as co-substrate
-
-
?
L-glutamine + NH2OH
?
-
10.3% of the activity with L-glutamine and H2O as co-substrate
-
-
?
nitrocefin + H2O
(2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
D-Asn + H2O
D-Asp + NH3
-
26% of the activity with L-Gln
-
-
?
D-asparagine + H2O
?
-
11% activity compared to L-glutamine
-
-
?
D-asparagine + H2O
D-aspartate + NH3
-
67% of the activity with L-glutamine
-
-
?
D-asparagine + H2O
D-aspartate + NH3
-
67% of the activity with L-glutamine
-
-
?
D-Gln + H2O
D-Glu + NH3
-
35% of the activity with L-Gln
-
-
?
D-glutamine + H2O
D-glutamate + NH3
-
24% activity compared to L-glutamine
-
-
?
D-glutamine + H2O
D-glutamate + NH3
-
24% activity compared to L-glutamine
-
-
?
D-glutamine + H2O
D-glutamate + NH3
-
48.5% activity compared to L-glutamine
-
-
?
D-glutamine + H2O
D-glutamate + NH3
-
48.5% activity compared to L-glutamine
-
-
?
D-glutamine + H2O
D-glutamate + NH3
-
8.1% of the activity with L-glutamine and H2O as co-substrate
-
-
?
D-glutamine + H2O
D-glutamate + NH3
-
75% of the activity with L-glutamine
-
-
?
D-glutamine + H2O
D-glutamate + NH3
-
75% of the activity with L-glutamine
-
-
?
gamma-glutamyl-methylamide + H2O
Glu + N-methylhydroxylamine
-
no activity
-
-
?
Gln + H2O
Glu + NH3
-
-
209009, 209010, 209012, 209013, 209015, 209016, 209017, 209018, 209019, 209028, 209029, 209030, 209031, 209033, 209034
-
-
?
L-Asn + H2O
L-Asp + NH3
-
77% of the activity with L-Gln
-
-
?
L-asparagine + H2O
?
-
93% activity compared to L-glutamine
-
-
?
L-asparagine + H2O
L-aspartate + NH3
reaction of EC 3.5.1.1
-
-
?
L-asparagine + H2O
L-aspartate + NH3
reaction of EC 3.5.1.1
-
-
?
L-asparagine + H2O
L-aspartate + NH3
-
74% of the activity with L-glutamine
-
-
?
L-asparagine + H2O
L-aspartate + NH3
-
74% of the activity with L-glutamine
-
-
?
L-gamma-glutamyl-4-nitroanilide + H2O
L-glutamate 4-nitroanilide + NH3
-
-
-
?
L-gamma-glutamyl-4-nitroanilide + H2O
L-glutamate 4-nitroanilide + NH3
-
-
-
?
L-gamma-glutamyl-methylester + H2O
L-Glu + methanol
-
no activity
-
-
?
L-Gln + H2O
L-Glu + NH3
-
enzyme plays a key role in urea synthesis by regulating provision of glutamate for synthesis of N-acetylglutamate, the obligatory cofactor of carbamoylphosphate synthetase
-
?
L-Gln + H2O
L-Glu + NH3
-
contribution of the enzyme to elevated extracellular Glu at 24 h after onset of focal ischemia
-
-
?
L-Gln + H2O
L-Glu + NH3
-
kidney enzyme increases during acidosis, skeletal muscle enzyme does not. Under some conditions the enzyme from skeletal muscle is able to catabolize Gln to Glu and further to CO2
-
-
?
L-Gln + H2O
L-Glu + NH3
-
high glutaminase activity may be of importance for optimal insulin secretion elicited by amino acid secretagogues
-
-
?
L-Gln + H2O
L-Glu + NH3
-
enzyme is involved in deamination of Gln to Glu, which is utilized for energy production via the TCA cycle. Glutaminase mRNA increases around the 3rd week of life
-
-
?
L-Gln + hydroxylamine
L-gamma-glutamyl hydroxamate + NH3
-
no activity
-
-
?
L-Gln + hydroxylamine
L-gamma-glutamyl hydroxamate + NH3
-
no activity
-
-
?
L-Gln + hydroxylamine
L-gamma-glutamyl hydroxamate + NH3
-
glutaminase A and B
-
?
L-glutamate-p-nitroanilide + H2O
L-glutamate + 4-nitroaniline
-
-
-
?
L-glutamate-p-nitroanilide + H2O
L-glutamate + 4-nitroaniline
-
-
-
?
L-glutamate-p-nitroanilide + H2O
L-glutamate + 4-nitroaniline
-
-
-
?
L-glutamate-p-nitroanilide + H2O
L-glutamate + 4-nitroaniline
Escherichia coli W3110 / ATCC 27325
-
-
-
?
L-glutamic acid + H2O
?
-
13.5% activity compared to L-glutamine
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
PDX2 is a glutaminase that is involved in vitamin B6 biosynthesis
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
biosynthesis of pyridoxal-5-phosphate
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
-
-
-
?
L-glutamine + H2O
L-glutamate + NH3
synthesis of neurotransmitter glutamate and regulates the concentrations of glutamine and glutamate, ammonia detoxification
-
-
?
L-glutamine + H2O
L-glutamate + NH3
glutamate is an important source for neurotransmitter
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
glutamine synthesis pathway, overview, the intestinal and brain phosphate-activated glutaminase plays a role in the pathogenesis of hepatic encephalopathy, overview, PAG is increased in cirrhotics showing minimal hepatic encephalopathy and, therefore, could be implicated in the production of systemic hyperammonemia in these patients, PAG localized into the astrocytes is responsible for ammonia and free-radical production, PAG regulation mechanism, overview
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
intestinal glutaminase activity is increased in liver cirrhosis and correlates with minimal hepatic encephalopathy, relationship between enzyme activity, liver function, and porto-systemic shunts, overview
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
the enzyme plays a key role in energy and nitrogen metabolism, glutamate is a major excitatory neurotransmitter in neurons, especially in the cerebral cortex
-
-
?
L-glutamine + H2O
L-glutamate + NH3
the glutamate bindig pocket of the enzyme involves Glu381 and Tyr249, structure overview
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
specifc for L-glutamine
-
-
?
L-glutamine + H2O
L-glutamate + NH3
strictly specific to L-glutamine
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
neurotransmitter glutamate has been thought to derive mainly from glutamine via the action of glutaminase type 1, the GLS1 pathway is essential for maintaining the function of active synapses, knockout mice lacking brain/kidney phosphate-activated glutaminase have impaired glutamatergic synaptic transmission, altered breathing, disorganized goal-directed behavior and die shortly after birth
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
renal ammonia genesis and the utilization of glutamine as a metabolic fuel, neuronal synthesis of glutamate
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
the enzyme plays a key role in energy and nitrogen metabolism
-
-
?
L-glutamine + H2O
L-glutamate + NH3
-
enzyme participates in an ATP-consuming cycle, plays a catabolic role in the degradation of glutamine to carbon skeletons, maintaining the optimal balance between glutamine and glutamate
-
-
ir
L-glutamine + H2O
L-glutamate + NH3
-
best substrate
-
-
?
nitrocefin + H2O
(2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
-
-
?
nitrocefin + H2O
(2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
Escherichia coli W3110 / ATCC 27325
-
-
-
?
additional information
?
-
-
no activity with glutamine and hydroxylamine as substrates, no formation of L-glutamate-gamma-monohydroxamate
-
-
?
additional information
?
-
-
the enzyme prefers free L-glutamine to free L-asparagine, C-terminal glutaminyl, and asparaginyl residues in peptides
-
-
?
additional information
?
-
-
the enzyme does not show any activity against L-Glu, D-Asn, L-Gln-Gly, gamma-glutamyl hydrazide, glutathione, and L-gamma-Glu-4-nitroanilide
-
-
?
additional information
?
-
-
the enzyme does not show any relative activity against L-asparagine, D-asparagine, and L-gamma-glutamyl-4-nitroanilide
-
-
?
additional information
?
-
-
the enzyme does not show any relative activity against L-asparagine, D-asparagine, and L-gamma-glutamyl-4-nitroanilide
-
-
?
additional information
?
-
the brain-specific BNIP-2-homology protein, BHIP-H is involved in enzyme regulation in neurons, loss of BNIP-H function could render glutamate excitotoxicity or/and deregulated glutamatergic activation, leading to ataxia, dystonia or other neurological disorders
-
-
?
additional information
?
-
-
the brain-specific BNIP-2-homology protein, BHIP-H is involved in enzyme regulation in neurons, loss of BNIP-H function could render glutamate excitotoxicity or/and deregulated glutamatergic activation, leading to ataxia, dystonia or other neurological disorders
-
-
?
additional information
?
-
-
multifunctional isozyme LGA interacts with PDZ domain proteins, e.g. alpha-1-syntrophin or glutaminase-interacting protein GIP, via specific short sequence motifs, interaction mechanism, overview
-
-
?
additional information
?
-
-
glutaminase as a potential component of the pathogenic process of human immunodeficiency virus associated dementia
-
-
?
additional information
?
-
-
involvement of essential Cys and His residues in the activity of isolated glutaminase from tumour cells
-
-
?
additional information
?
-
-
no hydrolysis of nicotinamide adenine dinucleotide, nicotinamide, and acetamide under the same experimental conditions as L-glutamine as substrate
-
-
?
additional information
?
-
-
no reaction with L-theanine, D-theanine, L-gamma-glutamylhydrazine, and L-gamma-glutamyl-p-nitroanilide
-
-
?
additional information
?
-
no reaction with L-theanine, D-theanine, L-gamma-glutamylhydrazine, and L-gamma-glutamyl-p-nitroanilide
-
-
?
additional information
?
-
-
the spatial segregation of tissue-specific isozymes in pancreas alpha- and beta-cells may have important functional implications, facilitating a differential regulation of glutamate production in insulin- and glucagon-secreting cells, overview
-
-
?
