Literature summary for 3.5.1.2 extracted from

Zhou, J.; Zhou, J.; Yang, H.; Yan, C.; Huang, F.
Characterization of a sodium-regulated glutaminase from cyanobacterium Synechocystis sp. PCC 6803 (2008), Sci. China C Life Sci., 51, 1066-1075.

Search for more literature for 3.5.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
-	Synechocystis sp. PCC 6803

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
12.2	-	L-glutamine	1 mol/l NaCl present	Synechocystis sp. PCC 6803
26.6	-	L-glutamine	Km apparent, no Michaelis-Menten enzyme	Synechocystis sp. PCC 6803

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Na+	1 mol/l: 2fold activity	Synechocystis sp. PCC 6803

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
35000	-	x * 35000, SDS-PAGE	Synechocystis sp. PCC 6803

Organism

Organism	UniProt	Comment	Textmining
Synechocystis sp. PCC 6803	P73903	gene slr2079	-

Purification (Commentary)

Purification (Comment)	Organism
-	Synechocystis sp. PCC 6803

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
29.7	-	1 mol/l NaCl present	Synechocystis sp. PCC 6803
31.3	-	-	Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamine + H2O	-	Synechocystis sp. PCC 6803	L-glutamic acid + NH3	-	?

Subunits

Subunits	Comment	Organism
?	x * 35000, SDS-PAGE	Synechocystis sp. PCC 6803

Synonyms

Synonyms	Comment	Organism
glutaminase	-	Synechocystis sp. PCC 6803

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	42	-	Synechocystis sp. PCC 6803

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.8	9.5	-	Synechocystis sp. PCC 6803

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Release 2023.1 (January 2023)