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Literature summary for 3.5.1.2 extracted from

  • Kumar, L.; Singh, B.; Adhikari, D.K.; Mukherjee, J.; Ghosh, D.
    A temperature and salt-tolerant L-glutaminase from Gangotri region of uttarakhand himalaya: enzyme purification and characterization (2012), Appl. Biochem. Biotechnol., 166, 1723-1735.
    View publication on PubMed
Search for more literature for 3.5.1.2

Inhibitors

Inhibitors Comment Organism Structure
NaCl enhances activity at 1-20% w/v, but reduces activity by 30% at 25% w/v, at 25% NaCl concentration, the enzyme retains 60% of its activity after 4 h Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
 L-glutamine pH 11.0, 70°C Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl enhances activity at 1-20% w/v, but reduces activity by 30% at 25% w/v, optimal at 1% Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 x * 66000, SDS-PAGE Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + H2O Bacillus sp. (in: Bacteria)
-
 L-glutamate + NH3
-
 ?
L-glutamine + H2O Bacillus sp. (in: Bacteria) LKG-01
-
 L-glutamate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
 MTCC 10401, isolated from Gangotri region of Uttarakhand Himalaya
-
Bacillus sp. (in: Bacteria) LKG-01
-
 MTCC 10401, isolated from Gangotri region of Uttarakhand Himalaya
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme, 49fold from cell-free extract by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration, to homogeneity Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
584.2
-
 purified enzyme, pH 11.0, 70°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glutamine + H2O low activity Bacillus sp. (in: Bacteria) D-glutamate + NH3
-
 ?
D-glutamine + H2O low activity Bacillus sp. (in: Bacteria) LKG-01 D-glutamate + NH3
-
 ?
L-glutamine + H2O
-
 Bacillus sp. (in: Bacteria) L-glutamate + NH3
-
 ?
L-glutamine + H2O preferred substrate Bacillus sp. (in: Bacteria) L-glutamate + NH3
-
 ?
L-glutamine + H2O
-
 Bacillus sp. (in: Bacteria) LKG-01 L-glutamate + NH3
-
 ?
L-glutamine + H2O preferred substrate Bacillus sp. (in: Bacteria) LKG-01 L-glutamate + NH3
-
 ?
L-glutaminyl-L-asparagine + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
L-glutaminyl-L-asparagine + H2O
-
 Bacillus sp. (in: Bacteria) LKG-01 ?
-
 ?
additional information no activity with D-asparagine Bacillus sp. (in: Bacteria) ?
-
 ?
additional information no activity with D-asparagine Bacillus sp. (in: Bacteria) LKG-01 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 66000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
L-glutaminase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 85 activity range Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified enzyme, 3 h, 100% stable Bacillus sp. (in: Bacteria)
70
-
 purified enzyme, 3 h, 50% activity remaining Bacillus sp. (in: Bacteria)
80
-
 purified enzyme, 3 h, 20% activity remaining Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11
-
-
 Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
5 12 activity range Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 11 purified enzyme, stable Bacillus sp. (in: Bacteria)