Cloned (Comment) | Organism |
---|---|
gene AoglsA, from strain RIB40, DNA and amino acid sequence determination and analysis, functional overexpression in Saccharomyces cerevisiae cell wall and in Escherichia coli as soluble protein | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49883 | - |
x * 49883, amino acid sequence calculation | Aspergillus oryzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | Aspergillus oryzae | - |
L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | Aspergillus oryzae RIB 40 | - |
L-glutamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | Q66V97 | - |
- |
Aspergillus oryzae RIB 40 | Q66V97 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 5.9fold from Escherichia coli by anion exchange, glutamine affinity, and hydrophobic interaction chromatography to homogeneity | Aspergillus oryzae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of different enzyme constructs recombinantly expressed in different Escherichia coli strains, overview | Aspergillus oryzae |
11.1 | - |
recombinant enzyme in Escherichia coli strain JM109 | Aspergillus oryzae |
730 | - |
purified recombinant enzyme | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | - |
Aspergillus oryzae | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | - |
Aspergillus oryzae RIB 40 | L-glutamate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49883, amino acid sequence calculation | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
AoGls | - |
Aspergillus oryzae |
Micrococcus luteus K-3-type glutaminase | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Aspergillus oryzae |