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3.5.1.2: glutaminase

This is an abbreviated version!
For detailed information about glutaminase, go to the full flat file.

Word Map on EC 3.5.1.2

Reaction

L-glutamine
+
H2O
=
L-glutamate
 +
NH3

Synonyms

AnsB, AoGls, GA, GAB, GAC, GahB, GLA, GLNase, GLS, GLS1, GLS2, GlsA, glutaminase, glutaminase 1, glutaminase 2, glutaminase A, glutaminase B, glutaminase C, glutaminase I, glutaminase K, glutaminase L, glutaminase-1, glutaminase-2, glutaminase-B, glutamine amidohydrolase, glutamine aminohydrolase, glutamine deamidating enzyme, K-glutaminase, KAG, KGA, kidney-type glutaminase, kidney-type-glutaminase, L-glutaminase, L-glutamine amidohydrolase, LAG, LGA, liver-type glitaminase, liver-type glutaminase, Mglu, Micrococcus glutaminase Mglu, Micrococcus luteus K-3-type glutaminase, mitochondrial glutaminase, N-PAG, neuroblastoma glutaminase, neuroblastoma PAG, Nit 2, nitrilase 2, omega-amidase, PAG, PDX2, phosphate activated glutaminase, phosphate-activated glutaminase, phosphate-activated L-glutamine amidohydrolase, salt-tolerant glutaminase, YaaE

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.2 glutaminase

Temperature Stability

Temperature Stability on EC 3.5.1.2 - glutaminase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 50
-
the enzyme retains relative stability up to 95% from 20 to 35°C and 50% from 40 to 50°C for 30 min. The stability of enzyme decreases sharply at higher temperature
35
-
30 min, pH 7.5, the enzyme from strain RIB40 is stable
37
-
pH 7.0, 93% loss of activity, stable in presence of 1 mM EDTA, 20% loss of activity in presence of 1 mM EGTA
4
-
low level of activity after partial purification at 4°C in absence of stabilizing ligands, warming at 24°C results in 2fold to 5fold increase in activity
40
-
pH 7.5, 10 min, 10% NaCl, glutaminase I and II stable up to
40 - 70
-
free and immobilized enzymes retain 85% of their activity during a 45 min of incubation at 40°C and 50°C. Both L-glutaminase enzyme lose about 5% of their original activity at 70°C after 45 min
50 - 70
-
the enzyme is stable under 55°C and retains 40% activity at 60°C for 1 h, while activity is drastically reduced at 70°C
80
-
purified enzyme, 3 h, 20% activity remaining
additional information
-
10% NaCl enhances thermal stability of glutaminase I