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3.5.1.2: glutaminase

This is an abbreviated version!
For detailed information about glutaminase, go to the full flat file.

Word Map on EC 3.5.1.2

Reaction

L-glutamine
+
H2O
=
L-glutamate
 +
NH3

Synonyms

AnsB, AoGls, GA, GAB, GAC, GahB, GLA, GLNase, GLS, GLS1, GLS2, GlsA, glutaminase, glutaminase 1, glutaminase 2, glutaminase A, glutaminase B, glutaminase C, glutaminase I, glutaminase K, glutaminase L, glutaminase-1, glutaminase-2, glutaminase-B, glutamine amidohydrolase, glutamine aminohydrolase, glutamine deamidating enzyme, K-glutaminase, KAG, KGA, kidney-type glutaminase, kidney-type-glutaminase, L-glutaminase, L-glutamine amidohydrolase, LAG, LGA, liver-type glitaminase, liver-type glutaminase, Mglu, Micrococcus glutaminase Mglu, Micrococcus luteus K-3-type glutaminase, mitochondrial glutaminase, N-PAG, neuroblastoma glutaminase, neuroblastoma PAG, Nit 2, nitrilase 2, omega-amidase, PAG, PDX2, phosphate activated glutaminase, phosphate-activated glutaminase, phosphate-activated L-glutamine amidohydrolase, salt-tolerant glutaminase, YaaE

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.2 glutaminase

Storage Stability

Storage Stability on EC 3.5.1.2 - glutaminase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for at least 1 year
-
0-5°C, 0.01 M potassium phosphate buffer, pH 7.2, containing 12.5% glycerol, little lose of activity
0-5°C, 0.01 M potassium phosphate buffer, pH 7.2, containing 12.5% glycerol, little loss of activity
0-5°C, pH 7.2, 10 mM Tris-HCl, 10% glycerol, 10 mM MgCl2, glutaminase I and II, stable for 1 month
-
30°C, pH 7.5, purified recombinant enzyme, stable for up to 6 h
4°C, free and immobilized enzyme at pH 8.0, 30 days, no loss of activity
-
4°C, in presence of sodium borate, stable for 5 days
-
4ºC, T-phosphate buffer, pH 8.0, 1 mM dithiothreitol, very stable
-
5°C, enzyme preparation as a lyophilized powder, indefinitely stable
-
stable at -196°C (unstable at -80°C)
stable at -80°C