General Stability | Organism |
---|---|
the activity of immobilized enzyme immobilized on polyacrylic acid-Cu2+-matrix decreases 1.5fold compared to the free enzyme | Trichoderma reesei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
L-glutamine | polyacrylic acid-Cu2+-immobilized enzyme, at pH 8.0 and 50°C | Trichoderma reesei | |
0.49 | - |
L-glutamine | free enzyme, at pH 8.0 and 50°C | Trichoderma reesei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | Trichoderma reesei | - |
L-glutamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma reesei | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36.45 | - |
polyacrylic acid-Cu2+-immobilized enzyme, at pH 8.0 and 50°C | Trichoderma reesei |
40.32 | - |
free enzyme, at pH 8.0 and 50°C | Trichoderma reesei |
Storage Stability | Organism |
---|---|
4°C, free and immobilized enzyme at pH 8.0, 30 days, no loss of activity | Trichoderma reesei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + H2O | - |
Trichoderma reesei | L-glutamate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-glutaminase | - |
Trichoderma reesei |
L-glutamine amidohydrolase | - |
Trichoderma reesei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Trichoderma reesei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | free and immobilized enzymes retain 85% of their activity during a 45 min of incubation at 40°C and 50°C. Both L-glutaminase enzyme lose about 5% of their original activity at 70°C after 45 min | Trichoderma reesei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Trichoderma reesei |