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3.4.24.B6: matrix metalloproteinase-20

This is an abbreviated version!
For detailed information about matrix metalloproteinase-20, go to the full flat file.

Word Map on EC 3.4.24.B6

Reaction

proteolytic cleavage of ameloblastin =

Synonyms

enamel metalloproteinase, enamel protease, enamelysin, M10.019, matrix metalloproteinase 20, MMP-20, MMP20

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.24 Metalloendopeptidases
                3.4.24.B6 matrix metalloproteinase-20

Substrates Products

Substrates Products on EC 3.4.24.B6 - matrix metalloproteinase-20

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
aggrecan + H2O
?
show the reaction diagram
cleavage site is Asn341-Phe342
-
?
ameloblastin + H2O
?
show the reaction diagram
ameloblastin + H2O
ameloblastin fragments
show the reaction diagram
amelogenin + H2O
23 kDa amelogenin + 5 kDa tyrosine-rich amelogenin peptide
show the reaction diagram
cleaves amelogenin between Trp45 and Leu46
-
-
?
amelogenin + H2O
?
show the reaction diagram
amelogenin + H2O
amelogenin fragment P148
show the reaction diagram
-
-
-
?
amelogenin + H2O
amelogenin fragments
show the reaction diagram
amelogenin + H2O
amelogenin fragments rH163 + rH146
show the reaction diagram
-
in high phosphates conditions (20.9 mM KH2PO4) no cleavage site is observed in the central region of residues Q54–P146, which also shows the slowest cleavage rate with the maximum accumulation of fragment rH163 at 4 h time-point. In the no calcium and phosphates conditions, the accumulation of rH163 reaches the maximum amount at 1 h and the cleavage in the central region Q54-P146 appears only after 5 h. Under high calcium conditions (33.4 mM CaCl2), the fastest cleavage rate is observed and also few exclusive and additional cleavage sites at T61, H67. Other conditions also show some exclusive cleavage sites such as P2 for no calcium and phosphates, P50 for low calcium and phosphates and G10 for high calcium and phosphates. The N-terminal positions Y11, N13, F14, S15, E17, K23, W24, P33, G42, and S53 and the C-terminal positions P155 and T158 are cleaved in all conditions used
-
?
amelogenin + H2O
fragments of amelogenin
show the reaction diagram
-
-
-
-
?
amylogenin + H2O
4 major peptide fragments of MWs 24 kDa, 23 kDa, 22 kDa, and 20 kDa
show the reaction diagram
-
-
?
amylogenin + H2O
?
show the reaction diagram
cartilage oligomeric matrix protein + H2O
60 kDa protein
show the reaction diagram
100 kDa protein substrate
-
?
casein + H2O
?
show the reaction diagram
dentin sialophosphoprotein + H2O
dentin sialoprotein-glycoprotein + dentin phosphoprotein
show the reaction diagram
-
-
-
-
?
dentin sialoprotein-glycoprotein + H2O
dentin sialoprotein + dentin glycoprotein
show the reaction diagram
-
-
-
-
?
E-cadherin + H2O
?
show the reaction diagram
-
MMP20 cleaves the extracellular domain of E-cadherin
-
-
?
enamel protein + H2O
?
show the reaction diagram
MMP-20 is secreted along with enamel proteins by secretory stage ameloblasts into the enamel matrix of developing teeth. Enamel protein cleavage products accumulate in the space between the crystal ribbons, helping to support them. MMP-20 steadily cleaves accumulated enamel proteins, so their concentration decreases with depth. The principle functions of MMP-20 in dental enamel formation is to facilitate the orderly replacement of organic matrix with mineral, generating an enamel layer that is harder, less porous, and unstained by retained enamel proteins
-
-
?
enamelin + H2O
enamelin fragments
show the reaction diagram
-
-
-
-
?
endostatin + H2O
peptides
show the reaction diagram
-
comparison of efficiency to several other proteinases
-
?
leucine-rich amylogenin peptide + H2O
leucine-rich amylogenin residues 181-186 + leucine-rich amylogenin residues 187-188
show the reaction diagram
corresponds to residues 181-188 of amylogenin, recombinant enzyme, cleavage site is P186-A187
-
?
Mca-KPLGL-Dpa-AR-NH2 + H2O
?
show the reaction diagram
-
-
-
?
Mca-PLA-norvaline-Dpa-AR-NH2 + H2O
?
show the reaction diagram
synthetic fluorogenic substrate
-
?
Mca-PLGL-Dpa-AR + H2O
?
show the reaction diagram
Mca-PLGL-Dpa-AR-NH2 + H2O
?
show the reaction diagram
Mca-PLGL-[3-DNP-2,3-DAP]-AR-NH2 + H2O
?
show the reaction diagram
Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 + H2O
?
show the reaction diagram
-
-
-
?
proteins + H2O
peptides
show the reaction diagram
type V collagen + H2O
?
show the reaction diagram
-
-
-
-
?
tyrosine-rich amylogenin peptide + H2O
tyrosine-rich amylogenin residues 36-45 + tyrosine-rich amylogenin residues 46-49
show the reaction diagram
corresponds to residues 36-49 of amylogenin, recombinant enzyme, cleavage site is W45-L46
-
?
dentin sialoprotein + H2O
additional information
-