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Literature summary for 3.4.24.B6 extracted from

  • Nagano, T.; Kakegawa, A.; Yamakoshi, Y.; Tsuchiya, S.; Hu, J.C.; Gomi, K.; Arai, T.; Bartlett, J.D.; Simmer, J.P.
    Mmp-20 and Klk4 cleavage site preferences for amelogenin sequences (2009), J. Dent. Res., 88, 823-828.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.24.B6

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Sus scrofa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amelogenin + H2O Sus scrofa
-
 amelogenin fragments
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from soft enamel-alkaline extract by anion exchange chromatography, ultrafiltration, and heparin affinity chromatography Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
enamel secretory stage Sus scrofa
-
tooth developing Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amelogenin + H2O
-
 Sus scrofa amelogenin fragments
-
 ?
amelogenin + H2O Mmp-20 cleaves amelogenin sequences after Pro162, Ser148, His62, Ala63, and Trp45. These cleavages generate all of the major cleavage products from tyrosine-rich amelogenin polypeptide that accumulate in porcine secretory stage enamel, i.e. the 23 kDa, 20 kDa, 13 kDa, 11 kDa, and 6 kDa amelogenins. And Mmp-20 cleaves leucine rich-amelogenin protein after Pro45 and Pro40, producing the two LRAP products, Mmp-20 cleavage site preferences, overview Sus scrofa amelogenin fragments
-
 ?
casein + H2O zymography Sus scrofa ?
-
 ?
additional information digestion of FRET-amelogenin peptides, labeled with 2-aminobenzoyl and 2,4-dinitrophenyl, by Mmp-20, overview Sus scrofa ?
-
 ?

Synonyms

Synonyms Comment Organism
enamel protease
-
 Sus scrofa
MMP-20
-
 Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Sus scrofa

General Information

General Information Comment Organism
physiological function the enzyme processes amelogenin to generate the major cleavage products that accumulate during the secretory stage of amelogenesis Sus scrofa