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Literature summary for 3.4.24.B6 extracted from
- Comparative properties of recombinant human and bovine matrix metalloproteinase-20 (2008), Arch. Oral Biol., 53, 785-790.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
| expressed in Escherichia coli BL21(DE3) cells | Bos taurus |
| expression in Escherichia coli | Homo sapiens |
| expression in Escherichia coli | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0143 | - |
Mca-PLGL-[3-DNP-2,3-DAP]-AR-NH2 | at 37°C | Bos taurus |  |
| 0.0143 | - |
Mca-PLGL-Dpa-AR | 37°C | Bos taurus | |
| 0.0164 | - |
Mca-PLGL-[3-DNP-2,3-DAP]-AR-NH2 | at 37°C | Homo sapiens | |
| 0.0164 | - |
Mca-PLGL-Dpa-AR | 37°C | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | - |
Homo sapiens | |
| Zn2+ | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS-PAGE | Homo sapiens |
| 55000 | - |
SDS-PAGE | Bos taurus |
| 55000 | - |
x * 55000, SDS-PAGE | Homo sapiens |
| 55000 | - |
x * 55000, SDS-PAGE | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Bos taurus | O18767 | - |
- |
| Homo sapiens | - |
- |
- |
| Homo sapiens | O60882 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
- |
Bos taurus |
| nickel resin chromatography | Homo sapiens |
| nickel resin chromatography | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| tooth | fetal tooth tissue | Homo sapiens | - |
| tooth | fetal tooth tissue | Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amelogenin + H2O | cleavage loci: FSY/EVL, GGW/LHH, MFP/MQP, MLP/DLT, DLT/LEA, LTL/EAW, AWP/STD | Homo sapiens | ? | - |
? | |
| amelogenin + H2O | cleavage loci: FSY/EVL, GGW/LHH, MFP/MQP, MLP/DLT, LTL/EAW, AWP/STD | Bos taurus | ? | - |
? | |
| amylogenin + H2O | - |
Homo sapiens | ? | - |
? | |
| amylogenin + H2O | - |
Bos taurus | ? | - |
? | |
| Mca-PLGL-Dpa-AR + H2O | - |
Homo sapiens | ? | - |
? | |
| Mca-PLGL-Dpa-AR + H2O | - |
Bos taurus | ? | - |
? | |
| Mca-PLGL-[3-DNP-2,3-DAP]-AR-NH2 + H2O | - |
Homo sapiens | ? | - |
? | |
| Mca-PLGL-[3-DNP-2,3-DAP]-AR-NH2 + H2O | - |
Bos taurus | ? | - |
? | |
| additional information | MMP-20 becomes functionally active when proteolytic cleavage removes the pro-domain to expose the zinc atom and free the active site for the enzyme to interact with its substrate | Homo sapiens | ? | - |
? | |
| additional information | MMP-20 becomes functionally active when proteolytic cleavage removes the pro-domain to expose the zinc atom and free the active site for the enzyme to interact with its substrate | Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE | Homo sapiens |
| ? | x * 55000, SDS-PAGE | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MMP-20 | - |
Homo sapiens |
| MMP-20 | - |
Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.1 | - |
Mca-PLGL-Dpa-AR | 37°C | Homo sapiens | |
| 8.1 | - |
Mca-PLGL-[3-DNP-2,3-DAP]-AR-NH2 | at 37°C | Homo sapiens | |
| 9 | - |
Mca-PLGL-Dpa-AR | 37°C | Bos taurus | |
| 9 | - |
Mca-PLGL-[3-DNP-2,3-DAP]-AR-NH2 | at 37°C | Bos taurus | |
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